Method for animal immunization by using needleless injector

Abstract

The invention discloses a method for immunizing animals by using a needleless injector, which comprises the following steps: S1, preparing an antigen mixed solution, the antigen mixed solution comprises DNA expression plasmid, CPG-ODN adjuvant and normal saline, and the DNA expression plasmid is used as a specific antigen; s2, performing needleless injection immunization on an immune animal, and injecting the prepared antigen mixed solution into the immune animal body by using a needleless injector, so that an immune reaction occurs in the immune animal body, and an antibody aiming at a specific antigen is generated; and S3, repeatedly performing needle-free injection immunization, and after 2-6 times of needle-free injection immunization, detecting the titer of the antibody aiming at the specific antigen in the immune animal serum. According to the immunization method provided by the invention, the immune response of animals is greatly improved, more opportunities are provided for research and development of monoclonal antibody drugs, the administration process is simplified by a needle-free injection immunization mode, the pain and complications of experimental animals are reduced, and the occupational safety of experimental operators can also be improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for animal immunization using a needle-free syringe. Background technique [0002] DNA immunity is a new immunology theory and technology established and developed in the early 1990s. Plasmid DNA can exist in recipient cells in a circular, non-integrated, non-replicating state for up to 1 month. Plasmid DNA can be directly taken up by cells in recipient animals and express gene product proteins, thus laying the foundation for DNA immunity. Through DNA immunization technology, the target gene DNA can directly produce antigens in the recipient animal, and stimulate the recipient animal body to produce an immune response to the target gene protein and produce antibodies. Animals with immune responses can then be used for animal serum antibody production, and can also be used for the development of monoclonal antibody drugs. [0003] The use of DNA immunization technology doe...

AI Technical Summary

Problems solved by technology

However, traditional veterinary needle-free syringes are usually large in size, expensive, cumbersome to operate, and require additional power gas as a power source, which is not conducive to the development of experiments and cannot meet the needs of a certain amount of experimental throughput

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