Enzymatic enhanced root uptake of agrochemically active compounds
A compound and active technology, applied in animal husbandry, animal repellent, plant growth regulator, etc., can solve the problems of low efficacy of pests, low concentration of plants, etc.
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[0301] In the following, a list of embodiments is provided to further illustrate the present disclosure and is not intended to limit the present disclosure to the specific embodiments listed below.
[0302] 1. A method of protecting plants or plant propagation material against attack or infestation by insects, fungi, arachnids or nematodes, against weeds, comprising by wetting the soil, applying to the soil by drip irrigation, by injecting the soil, The following are applied directly and / or indirectly to plants and / or plant propagation material by dipping, furrow application or by treating the plant propagation material:
[0303] at least one active compound; and
[0304] At least one enzyme selected from the group consisting of proteases, peptidases, cellulases, hemicellulases, pectinases, chitinases, chitosanases, lipases, cutinases, esterases, and xylanases.
[0305] 2. At least one active compound and selected from the group consisting of protease, peptidase, cellulase, h...
Embodiment 1
[0434] Cutinase p-(3R)-3-(2-chlorothiazol-5-yl)-8-methyl-5-oxo-6-phenyl-2,3-dihydrothiazole in Canola do[3,2-a]pyrimidine-8- Influence of absorption of -7-alkoxides.
[0435] Material:
[0436] Active ingredient / active compound: (3R)-3-(2-chlorothiazol-5-yl)-8-methyl-5-oxo-6-phenyl-2,3-dihydrothiazolo[3,2 -a]pyrimidine-8- -7-alkoxide
[0437] Enzymes:
[0438] Cutinase of CUT-1 - SEQ No. 1
[0439] Cutinase of CUT-2-SEQ No. 2
[0440] Cutinase of CUT-4-SEQ No. 4
[0441] Cutinase of CUT-9 - SEQ No. 9
[0442] General Seed Treatment (ST) Methods:
[0443] Canola seeds were treated with the formulated product diluted in water by spinning 100 g of seeds in a 1 liter HEGE bowl while applying the treatment slurry. The seeds are spun to dry.
[0444] General approach to Coating Efficiency:
[0445] To obtain coating efficiency, 100 treated seeds were randomly selected and then divided into 10 groups. Each group (10 seeds as a group) was analyzed separately by LC / MS. ...
Embodiment 2
[0457] Effects of Cutinases on Clothianidin Uptake in Seed-treated Wheat Seedlings
[0458] Active ingredient / active compound: clothianidin
[0459] Enzymes:
[0460] Cutinase of CUT-1 - SEQ No. 1
[0461] Cutinase of CUT-2-SEQ No. 2
[0462] Cutinase of CUT-4-SEQ No. 4
[0463] other materials:
[0464] RediEarth is a potting mix
[0465] experimental method
[0466] a. Seed Treatment: Dilute the formulation and dissolve the enzyme in HPLC water. Prepare and mix solutions immediately before each treatment. For each treatment, 100 g of spring wheat seeds (Triticum sp.) were placed in a 1 liter HEGE 11 seed processor. The treatment slurry was applied to the spinning seeds in an application volume of 1.5 mL (containing 2.05 mg of cell extract containing lyophilized enzymes) and the seeds were spun to dryness. After treatment, transfer the seeds to paper seed bags and store in a cooler field with an ice pack until planting.
[0467]b. Plant Incubation: Before seed tre...
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