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Method for building virus hepatitis mice model

A viral hepatitis and mouse model technology, which is applied in animal husbandry and other fields, can solve the problems of lack of pathogen replication and differentiation, and achieve low cost, overcome limitations, and good repeatability

Inactive Publication Date: 2004-12-29
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology

However, the model still suffers from the limitation of lack of pathogen replication in vivo, which is significantly different from the clinical situation

Method used

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  • Method for building virus hepatitis mice model

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Embodiment Construction

[0018] Below in conjunction with specific embodiment the present invention is described in further detail:

[0019] The method for setting up the viral hepatitis mouse model proposed by the present invention comprises the following steps:

[0020] 1) Preparation of different strains of mice: 12 as a group, select several groups of BALB / CJ pure-line female mice, several groups of C3H / HEJ pure-line female mice and several groups of A / J pure-line female mice , reared in the animal breeding laboratory for later use. The age of pure female mice in the above groups is preferably in the range of 6-8 weeks of gestational age, because the symptoms reflected by mice in this age range after infection with hepatitis virus are very stable, and it is easy to simulate the infection situation of humans, while The best age for the selected mice should be 8 weeks of gestational age. The animal grades of the pure-line female mice in each of the above groups should be clean, so as to prevent th...

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Abstract

The present invention is the method of establishing mouse model of viral hepatitis. The method includes selection of mouse hepatitis virus-III (MHV-3) of 100-1000 pFu, injecting infection of variousvariety mice of BALB / CJ, C3H / HEJ and A / J mice, and observing the survival rate and liver tissue change. Via the said process, three kinds of viral hepatitis mouse model are established, of fulminanthepatitis, chronic hepatitis and symptom-free mouse separately. The establishment of the model provides powerful facilities for systematic research of viral hepatitis in genetic regulation, molecular mechanism and prevention and treatment.

Description

technical field [0001] The invention relates to a method for establishing a mouse model of viral hepatitis, which is used for simulating, researching and preventing and treating human viral hepatitis. Background technique [0002] Viral hepatitis, especially hepatitis B virus, is an important disease that endangers human health. According to preliminary estimates, there are about 400-500 million carriers of hepatitis B virus (HBV) in the world. my country is a high-incidence area for hepatitis B virus infection, and the rate of people carrying hepatitis B virus is about 10-20%. There are more than 50,000 cases of fulminant hepatitis caused by viruses every year in the world, and the mortality rate is as high as 80-90%. The molecular basis of liver cell necrosis in viral hepatitis is still unclear, and there is no effective treatment. Therefore, in-depth research on the molecular immune mechanism of viral hepatitis liver cell necrosis can provide a the...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027
Inventor 宁琴罗小平严伟明郝连杰
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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