Target directional confluent DNA vaccine for preventing caries and preparation method thereof

A DNA vaccine, anti-caries technology, applied in recombinant DNA technology, DNA/RNA fragments, drug combinations, etc., can solve problems such as non-compliance with DNA vaccine requirements

Inactive Publication Date: 2006-09-06
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the vector pCI plasmid of the vaccine contains the ampicillin resistance gene, which does not meet the relevant requirements for the vector backbone of DNA vaccines

Method used

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  • Target directional confluent DNA vaccine for preventing caries and preparation method thereof
  • Target directional confluent DNA vaccine for preventing caries and preparation method thereof
  • Target directional confluent DNA vaccine for preventing caries and preparation method thereof

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Experimental program
Comparison scheme
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Embodiment Construction

[0055] The present invention will be described in further detail below according to accompanying drawing:

[0056] according to figure 1 , figure 2 It can be known that using molecular biology professional software: DNASIS comprehensive DNA sequence analysis software (Hitachi, Japan), Gene Construction kit 2.0 gene simulation cloning software (Textco, USA) to analyze and design a caries-preventing DNA vaccine with a new vector targeting fusion and simulate cloning The whole process. The whole process of constructing pGJA-P / VAXA new vector targeted fusion anti-caries DNA vaccine: Digestion with restriction enzymes NheI and NotI to treat targeted fusion anti-caries eukaryotic expression plasmid pGJA-P and pVAX1 vector; electrophoresis digestion product, Cut out the target fusion gene (including CTLA-4-Ig target gene, GLU, A-P fragment) and the pVAX1 vector fragment treated with NheI and NotI digestion, gel recovery and purification of the target fragment; the above two purifi...

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Abstract

The invention discloses a target directional confluent DNA vaccine for preventing caries and preparation method thereof, i.e. Escherichia coli JM109 / pGJA-P / VAX, CCTCC No:M204028, the preparation comprises cutting out targeting fusion genes (including CTLA-4-Ig destination gene, GLU, A-P fragment) from the fusion dental caries proofing eucaryon expression plasmid pGJA-P with limited enzyme NheI and NotI enzyme cutting treatment, cloning targeting fusion gene to pVAX1 carrier, enzyme cutting has shown the correctness of the construction, which is named pGJA-P / VAX, the transfection of eukaryotic cells CHO cell lines has confirmed that right expression recombination protein can expressed in the eukaryotic cells.

Description

technical field [0001] The invention belongs to the technical field of oral preventive medicine, and more specifically relates to a sequence encoding human cytotoxic T lymphocyte antigen 4 signal peptide, extracellular region and Igγ 1 A DNA anti-caries vaccine containing a novel carrier of hinge region, constant region fusion gene, surface protein antigen gene and glucosyltransferase gene of Streptococcus mutans, and the present invention also relates to the preparation of a targeted fusion anti-caries DNA vaccine method. Background technique [0002] Dental caries is a common and frequently-occurring disease that seriously endangers the health of human oral cavity and the whole body. The loss of function, inflammation, pain, and organ diseases caused by dental caries can seriously affect the quality of life, reduce the health quality of the population, and bring great harm to society. Caries is a bacterial infectious disease, and Streptococcus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K39/00C12N15/62A61P1/02
Inventor 樊明文贾荣郭继华边专陈智彭彬范兵
Owner WUHAN UNIV
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