Floppers herb flavone compound LEF
A technology of rooting and rooting on the ground, which is applied to the preparation of sugar derivatives, sugar derivatives, and sugar derivatives, and can solve problems such as retention
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[0035] 1. Resin pretreatment
[0036] (1) Macroporous Adsorption Resin Put the resin into the column, rinse with acetone 2 to 3 times (volume ratio of liquid to solid) first, then wash with deionized water 3 to 4 times (volume ratio of liquid to solid), and then continue to use Wash with 1mol / L hydrochloric acid aqueous solution with the same volume as the resin, and let it stand for 8 to 12 hours after completion, wash the column with deionized water to neutrality, continue to wash the column with 1mol / L NaOH solution until the effluent is strongly alkaline, after completion After standing still for 3-12 hours, wash the column with deionized water until neutral.
[0037] (2) Polyamide Take polyamide powder and soak it in 90-95% ethanol aqueous solution with a volume of 1-2 times (solid-liquid volume ratio), stir constantly, remove air bubbles, and put it into a chromatography column. Wash with 3-4 times (solid-to-liquid volume ratio) volume of 90-95% ethanol aqueous solution...
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