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Formulation of aweto culture medium and oxygenating culture method of aweto

A cultivation method and culture medium technology are applied in the field of strain and fruiting body cultivation matrix formulations of Cordyceps militaris, which can solve the problems of limited production efficiency of Cordyceps militaris, low nutritional and medicinal value, slow growth of fruiting bodies, etc. Production efficiency, easy to promote and use, uniform growth effect

Inactive Publication Date: 2007-05-02
袁有宝 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] There are many problems and deficiencies in the prior art of the above content. For the cultivation process of Cordyceps militaris, there are places that need to be improved and perfected from the liquid strain culture to the growth and development of the fruiting body, especially there is no effective measure for regular supplementary oxygenation. , so the production efficiency of Cordyceps militaris is limited, the growth of fruit bodies is slow, uneven, and the nutritional and medicinal value is not high

Method used

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  • Formulation of aweto culture medium and oxygenating culture method of aweto
  • Formulation of aweto culture medium and oxygenating culture method of aweto

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Embodiment 1

[0059] Embodiment 1, in conjunction with Fig. 1 and Fig. 2, described Cordyceps militaris culture medium formula, is in the culture medium solution that the pure strain that obtains after spore separation or tissue separation obtains, provides for the realization of deep expansion and rejuvenation culture. breeding substrate.

[0060] The formula of the culture medium is, by weight ratio of the ingredients, 2% of glucose, 1.5% of peptone, 1.5% of yeast powder, 0.005% of potassium dihydrogen phosphate, 0.002% of magnesium sulfate, 0.5% of sodium chloride, and 0.002% of vitamin B1 -0.005%, the rest is water.

[0061] Cordyceps militaris aerobic cultivation method of the present invention, its realization flow process is:

[0062] The first step is to prepare strains.

[0063] The fruiting body of wild Cordyceps militaris is collected for isolation, purification and cultivation. Spore isolation is used to inoculate the obtained bacteria beads on the medium for expansion and cul...

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Abstract

The present invention is formulation of aweto culture medium and oxygenating culture method of aweto. By means of high efficiency culture medium, deep culture of the liquid spawn and the oxygenating in the initial growth stage of sporophore, the present invention has high aweto sporophore producing efficiency and high yield. The culture medium for liquid spawn, which is obtained through spore or tissue separation and inoculated to culture medium solution, consists of glucose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate, sodium chloride, vitamin B1 and water in certain weight proportion.

Description

technical field [0001] The invention relates to a strain of Cordyceps militaris and a formula for cultivating a sporocarp, and a method for increasing growth and rejuvenation by increasing oxygen and promoting production by supplementing oxygen during the cultivating process. Background technique [0002] With the improvement of living conditions, more and more attention is paid to the improvement of quality of life and physical health, and various nutritional supplements have also become the leading products in the health care market. Cordyceps militaris is a precious Chinese medicinal material with high medicinal and nourishing effects. The distribution of wild Cordyceps is scarce and resources are extremely scarce. In order to meet the market demand, existing Cordyceps are artificially cultivated. Moreover, the active ingredients of artificially cultivated Cordyceps are close to natural, and some components are even several times or dozens of times higher than wild Cordy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 袁有宝
Owner 袁有宝
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