Blending culture for sponge and intergrowth microbe, culturing colony monitoring method

Abstract

The mixed cultivation and culturing population monitor method for sponge symbiotic microbe comprise: cleaning the epiphytic microbe on sponge surface, dipping into artificial sea water to shock and release symbiotic microbe in sponge for preparing germ liquid; extracting and preparing sponge draw-off juice with alcohol to add into seawater culture medium and prepare composite medium; inoculating to culture mixed microbe; extracting the whole DNA of cultured microbe to amplify into 16S rDNA segment less than 500bp by PCR for modified gradient gel electrophoresis and obtaining gene fingerprint pattern; realizing in-vitro monitor by callbacking fingerprint pattern strips of different time, cloning, sequencing, and homological and phylogenetic analysis to identify germ kind. This invention can improve separation and culture efficiency, and provides opposite material and technique support.

Description

technical field [0001] The invention relates to a method for monitoring the mixed culture of sponge symbiotic microorganisms and cultivable populations, in particular to the monitoring of the mixed culture of sponge symbiotic microorganisms and the population structure of cultivable microorganisms in the presence of sponge extracts. It belongs to the technical field of marine microbial engineering. Background technique [0002] Sponges are one of the marine organisms with the most abundant marine active substances, but due to source limitations, few active substances have entered the clinical research stage. At present, more and more studies have shown that some active substances with medicinal value are produced by sponge symbiotic microorganisms. Therefore, using microbial fermentation engineering technology may be the most feasible way to prepare sponge active substances in large quantities. [0003] The traditional microbial fermentation culture is based on pure bacter...

AI Technical Summary

Problems solved by technology

[0007] For the dynamic monitoring of microbial populations in the process of mixed microbial fermentation culture, the current conventional technology is still difficult to achieve

16S rDNA-DGGE (denaturing gradient gel electrophoresis) gene fingerprint technology has become a powerful tool to reveal the structure of complex microbial flora, and is also very suitable for the dynamic detection of microbial population structure over time in the process of mixed microbial culture. Any report on the application of technology to monitoring the population of sponge symbiotic microorganisms in the mixed culture process

Images