Method and culture medium for carrying out induction culture on regenerated plants by adopting pulsatilla chinensis anthers
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for inducing culture medium and regenerating plants, which is applied in the field of inducing and cultivating regenerated plants of Pulsatilla anthers, and achieves the effects of high induction rate and improved culture efficiency.
Active Publication Date: 2018-02-23
FORESTRY RES INST OF HEILONGJIANG PROVINCE
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] There are few reports on the in vitro cultivation of Pulsatillae, mainly focusing on explants such as seeds and leaves for in vitro culture. There is no report on how to use Pulsatilla pollen as explants to successfully cultivate Pulsatilla regenerated plants in vitro
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0078] The basal medium used for Pulsatilla anther-induced regeneration plants is MS modified medium, which consists of:
[0081] Iron salt: disodium edetate (Na 2 EDTA) 37.3mg / L and ferrous sulfate (FeSO 4 ·7H 2 O) 27.8mg / L;
[0082] Organic Matter: Inositol (C 6 h 12 o 6 2H 2 0) 100mg / L, Niacin (NC 5 h 4 COOH) 0.5mg / L, thiamine hydrochloride (C 12 h 17 ClN 4 OS·HCl) 0.1mg / L, pyridoxine hydrochloride (C 8 h 11 o 3 N·HCl) 0.5mg / L and glycine (NH 2 ·CN 2 · COOH) 2mg / L.
Embodiment 2
[0083] Example 2 Pulsatilla pollen induced culture regeneration plant
[0084] 1. Material collection and processing
[0085] From April to May, the fine wild varieties of Pulsatilla pulsatillae in Maoer Mountain, Harbin City, Heilongjiang Province were selected. At this time, the pollen is in the vigorous growth period, and the collection time is selected from 9 to 10 am in sunny weather. During the period from budding (emergence of flower buds) to flowering, flower buds were collected every 2 days. The collected flower buds were placed in a refrigerator at 4°C for 48 hours at low temperature and set aside.
[0086] Before inoculation, the stage of pollen development was identified by microscopic examination, and the pollen of Pulsatilla grandis in the middle stage of mononuclei was selected.
[0087] The microscopic examination method for determining the pollen development period is as follows: tear off the corolla with tweezers and take out the anthers, place the anthers ...
Embodiment 3
[0093] The culture medium and steps for inducing callus were the same as in Example 2, except that the developmental stage of the anthers was selected as the uninucleate marginal stage.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention provides a basic culture medium for carrying out induction culture on regenerated plants by adopting pulsatilla chinensis anthers, and belongs to the technical field of culturing of plant tissues. Using amounts of macroelements and a part of trace elements are improved based on an MS culture medium. Based on the basic culture medium provided by the invention, induction culture on theregenerated plants by adopting the pulsatilla chinensis anthers is carried out, and induction is achieved successfully so as to obtain the regenerated plants of the pulsatilla chinensis anthers. Theinvention also provides a method for carrying out induction culture on the regenerated plants by adopting the pulsatilla chinensis anthers. The method comprises the following steps: selecting and inoculating the pulsatilla chinensis anthers in monocyte periods into the induced culture medium; performing dark culture until green and bright shoots are formed on the surfaces of wound callus, transferring the shoots into a proliferation culture medium for proliferation culture, and carrying out induction to form clustered shoots; transferring the clustered shoots into a rooting culture medium forculturing to obtain the regenerated plants of the pulsatilla chinensis. The pulsatilla chinensis anthers are cultured by adopting the method provided by the invention, so that an induction ratio, a proliferation ratio and a rooting ratio are high, and culture time is short, and the culturing efficiency of the regenerated plants of the pulsatilla chinensis is effectively improved.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a basal medium, an induction medium, a proliferation medium and a rooting medium for inducing and cultivating regenerated plants of Pulsatilla pulsatillae anthers, and a method for inducing and cultivating regenerated plants of Pulsatilla chinensis anthers. Background technique [0002] Pulsatilla chinensis (Bunge) Regel, also known as sheep's beard flower, old crown flower, general grass, big bowl flower, husband flower, old sister-in-law flower and Maoguduo flower, is a plant of the genus Pulsatilla in the family Ranunculaceae. Plant height 15-35 cm, rhizome 0.8-1.5 cm thick, basal leaves 4-5, leaves broadly ovate, 3-lobed, middle segment stalked or nearly sessile, scape 1(-2), Plumber, erect flowers, sepals blue-purple, oblong-ovate, aggregate fruit 9-12 cm in diameter, achenes spindle-shaped, flat, flowering from April to May. Pulsatilla mostly grows in areas wi...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more