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In-vitro liver culture method

An in vitro culture and liver technology, applied in the biological field, to avoid the risk of infection, reduce shear force damage, and avoid damage

Inactive Publication Date: 2016-08-10
HEFEI HUAQI BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention is how to simulate the physiological environment of the liver so as to realize the in vitro culture of the liver

Method used

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  • In-vitro liver culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Using the method of the present invention to culture adult SD rat liver in vitro.

[0063] 1. Disinfect the abdomen of the rat with povidone iodine for 3 times, cover the rat with a clean and sterilized small hole towel, expose the abdomen, and cover with the hole towel. set up surgical instruments;

[0064] 2. Open the abdominal cavity, clamp the skin and the drape with hemostats, turn them to both sides, find out the portal vein and bile duct with dressing forceps, clamp the intestines at the bile duct with hemostats, and bury the bile duct with a curved needle Line, cut the bile duct, insert the epidural catheter, tie the embedded line tightly, adjust the position and angle of the tube, and let the bile flow out naturally;

[0065] 3. Strip the abdominal aorta and main vein from the spine, embed the thread, separate the abdominal aorta from the main vein with dressing forceps and elbow forceps, cut off the flow with a hemostat, and cut the abdominal aorta ...

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Abstract

The invention relates to an in-vitro liver culture method which comprises the steps of in-situ liver perfusion, liver separation and in-vitro liver culture. In-situ liver perfusion is achieved through the step that in-situ perfusion is conducted on the liver through a homemade culture solution under the low-temperature condition in a gravity perfusion mode; when liver separation is conducted, the separated liver is acquired through a conventional liver separation operation on the premise that perfusion is continuously conducted; in-vitro liver culture is achieved through the steps that the in-vivo physiological environment, including the circulating mode, the liver perfusion pressure, the liver perfusion amount, the oxygen supply mode, the stable acidity and alkalinity, the temperature constancy and the mechanical conditions, of the liver is simulated, and the separated liver is cultured through a homemade in-vitro liver culture system. Accordingly, a method is supplied to the research that the young liver is cultured into the adult liver by simulating the physiological conditions of the liver.

Description

technical field [0001] The invention belongs to the field of biotechnology, and is mainly used for in vitro culture of mammalian liver, especially for in vitro culture of rat and miniature pig liver. Background technique [0002] At present, the only effective treatment for end-stage liver disease at home and abroad is liver transplantation, and in China and even in the world, the sources of liver transplant donors are extremely scarce. At present, no matter how the liver preservation technology develops, it cannot fundamentally solve the problem of the extreme imbalance between the supply and demand of liver transplantation donors. Therefore, liver organ culture has become a new way to open up the source of liver transplantation donors. [0003] At present, in the existing technology, liver organ culture still exists at the level of cell mass tissue culture, such as US Patent US20080193421 A1 and Chinese Patent CN102391983B, which are three-dimensional culture in name, but...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0671C12N2500/30C12N2500/32C12N2500/36C12N2500/84C12N2503/00C12N2509/10C12N2523/00
Inventor 许健健许莉莉其他发明人请求不公开姓名
Owner HEFEI HUAQI BIOLOGICAL ENG CO LTD
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