Method for tissue culturing ice edge plant in high mountains and for production of excreting protein-from cold induction

A technology of plant tissue culture and protein secretion, which is applied in the field of protein engineering, can solve the problems of difficult technology mastery and limitation of antifreeze protein separation, and achieve the effects of improving survival rate, narrowing the candidate range, and reducing transpiration and dehydration

Inactive Publication Date: 2007-07-18
LANZHOU UNIVERSITY +1
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  • Summary
  • Abstract
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Problems solved by technology

Due to the non-universal distribution of antifreeze protein, it is difficult to find it from several specific plants, and the traditional apoplast extraction technology is difficult to master, which greatly limits the isolation of antifreeze protein secreted by cold induction

Method used

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  • Method for tissue culturing ice edge plant in high mountains and for production of excreting protein-from cold induction
  • Method for tissue culturing ice edge plant in high mountains and for production of excreting protein-from cold induction

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Embodiment Construction

[0026]The technical scheme of the present invention is described in further detail below in conjunction with the accompanying drawings: (1) alpine ice source plant sample collection. In August, samples were taken from an empty ice bucket near Tianshan No. 1 Glacier. There is a temperature difference between freezing and thawing repeatedly during the day and night. The average temperature during the day is about 10°C, and the average temperature at night is below 0°C. Utilize the collecting rod to dig the plant of the plant with the root and the soil, and quickly place it in the polyethylene collection bag. The collected plants are taken out and put into the transport box 1 on the same day, and the slow-release ice bag 2 is placed on the interlayer support partition 3, every After 6 hours, water was sprayed into the box from the ventilation hole, and at the same time, several leaves of each plant were fixed with FAA fixative solution at the sampling site, and reserved for morpho...

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Abstract

A method for the tissue culture of alpine perglacial plant and preparing the cold-induced secretoprotein includes sampling, determining the candidate standard, screening the plant, tissue culture of the leaf coming from the apline periglacial plant, pretreating by hormone GA3 for de-differentiating, enlargig reproduction, differentiating again, evaluating its variation, cold inducing by shake culture of suspended tissue, testing the secretoprotein, and separating the protein.

Description

technical field [0001] The invention belongs to protein engineering technology, in particular to a method for alpine periglacial plant tissue culture and cold-induced secretion protein production. Background technique [0002] Plant secreted protein refers to the protein secreted by plant cells into the apoplast, which can be secreted into the culture medium during liquid shaking culture. Common plant secreted proteins include signal polypeptides or antifreeze proteins. For example, calmodulin is a signal polypeptide. It has been confirmed by more than 10 years of research at home and abroad that calmodulin not only exists outside the plant cells, but also has the ability to promote the proliferation of suspension culture cells and their protoplasts and promote the growth of secondary cells in the extracellular site. Phosphoribulose carboxylase / oxygenase small subunit gene light-independent expression and other important biological functions; the research on antifreeze prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/34A01H4/00A01H3/00
Inventor 安黎哲王建辉
Owner LANZHOU UNIVERSITY
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