Simple preserving method of edible and medicinal mushroom strains

A technology for edible and medicinal fungi and strains, which is applied in the fields of preservation of microorganisms, microorganism-based methods, biochemical equipment and methods, etc. Good silk consistency

Inactive Publication Date: 2020-07-28
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These preservation methods do have advantages for the long-term preservation of strains, but they are extremely inconvenient in production and circulation, requiring special protective devices or containers for transportation

Method used

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  • Simple preserving method of edible and medicinal mushroom strains
  • Simple preserving method of edible and medicinal mushroom strains
  • Simple preserving method of edible and medicinal mushroom strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Prepare a square or round filter paper with a specification of 1cm*1cm or 2cm*2cm (limited to medium-speed quantitative filter paper, which is convenient for the attachment of mycelium), sterile tweezers, self-made sterile coated (PE) kraft paper envelopes, Put it on the ultra-clean workbench for standby;

[0032] 2. Prepare the nutrient medium required for the preparation of edible and medicinal fungus strains, sterilize under high temperature and high pressure (such as 121°C, 20min), then pack them into plates with a diameter of 9cm in an ultra-clean workbench, cool and solidify for later use;

[0033] 3. Use a 0.5cm aseptic puncher to uniformly punch the cultivated plate strains to prepare bacteria blocks with a diameter of 0.5cm. figure 1 Evenly inoculate the marked positions on the new PDA plate culture medium, and then use sterile tweezers to spread the sterile filter paper around the bacterial block, keep an appropriate distance (about 0.5cm) between the filte...

Embodiment 2

[0043] Embodiment 2: take filter paper sheet as the bacterial classification of preservation medium to use

[0044] 1. Prepare sterile tweezers, sterile scissors, sterile medium (plate or test tube);

[0045] 2. Use 75% alcohol to sterilize the surface of the small paper bag containing the bacteria, transfer it to the ultra-clean workbench, use sterile scissors to cut the seal of the bag, and then use sterile tweezers to take out the filter paper covered with mycelia, and then to a plate or test tube culture medium;

[0046] 3. Transfer the inoculated plate or test tube culture medium to an artificial climate incubator, and incubate at a suitable temperature in the dark until the mycelia cover the plate or test tube, then take it out for use.

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Abstract

The invention relates to preservation of edible mushroom strains, in particular to a simple preserving method of edible and medicinal mushroom strains. The simple preserving method comprises the stepsof (1) inoculating strains to a plate culture medium, uniformly drilling plat strains which are cultured through a bacteria-free hole puncher, preparing strain blocks being 0.4-0.5cm in diameter, anduniformly inoculating the strain blocks to a new plate culture medium; (2) placing filter paper sheets around the strain blocks, and maintaining distances between the filtering sheets; (3) performingplate culturing after inoculating until mycelium completely covers the filter paper sheets; (4) gently uncovering the filter paper sheets covered with the edible and medicinal mushroom mycelium basedon the condition that the culture medium is not stuck, and performing transferring to a bacteria-free kraft paper envelope; and (5) putting the kraft paper envelope loaded with the mycelium filteringpaper sheets in discoloured silica gel performing drying, performing normal-temperature placing in a sealed container for 10-15 days, then sealing the kraft paper envelope, and performing moving to 4DEG C for short-time preservation or express circulation. The method is simple and easy to operate, good in mycelium consistency, used for short-term preservation, and favorable for follow-up study.

Description

technical field [0001] The invention relates to the preservation of edible fungus strains, in particular to a convenient preservation method for edible and medicinal fungus strains. Background technique [0002] Edible and medicinal fungus strains are an important cornerstone of the development of the edible and medicinal fungi industry, and the quality of the strains directly affects the yield and quality of the fruiting bodies of edible and medicinal fungi. At present, the preservation methods of edible and medicinal fungus strains mainly include subculture low-temperature preservation and ultra-low temperature (liquid nitrogen) preservation. base) mainly. These preservation methods do have advantages for the long-term preservation of strains, but they are extremely inconvenient in production and circulation, requiring special protective devices or containers for transportation. Contents of the invention [0003] In view of the above deficiencies, the present invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/04C12R1/645
CPCC12N1/14C12N1/04
Inventor 吴晓贤刘远超胡惠萍莫伟鹏卓丽君谢意珍张智
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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