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Methods and compositions for selective cancer chemotherapy

A composition and selective technology, applied in the field of tumor cytotoxic chemotherapy, can solve the problems of vitamin C excretion, establishment and maintenance of high serum level of vitamin C, no vitamin C storage mechanism, etc.

Inactive Publication Date: 2002-01-02
易思特C公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no storage mechanism for vitamin C in human tissues and all vitamin C is metabolized and / or excreted
Further, it is difficult to establish and maintain high serum levels of vitamin C by oral administration of ascorbic acid due to gastrointestinal complications

Method used

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  • Methods and compositions for selective cancer chemotherapy
  • Methods and compositions for selective cancer chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Test procedure Cell lines: Malme-3M human melanoma (ATCC code HTB-64) Malme-3 normal human skin fibroblasts (ATCC code HTB-102) SK-Hep-1 human liver adenocarcinoma (ATCC code HTB-52 )WRL normal human hepatocytes (ATCC code CL-98) SK-N-MC human neuroblastoma (ATCC code HTB-10) T-84 human colon carcinoma (ATCC code CCL-248) storage cells in growth medium Cultivate as follows:

[0031]Cell Line Growth Medium SK-Hep-1, SK-N-MC and Eagle's Minimal Essential Medium WRL 88 Medium in Earle's Salts Supplemented with 2mM L-Glutamine, 1mM Sodium Pyruvate, 10% Fetal bovine serum (FBs) and antibiotics (penicillin, streptomycin, amphotericin B) Malme-3 and Malme 3-M McCoy's medium, plus L-glutamine, 15% FBs and antibiotics T-84 Dulbecco Ham's modified MEM and Ham's F-12 medium were mixed 1:1, supplemented with L-glutamine, pyridoxal hydrochloride, 25mM Hepes plus 5% FBS and antibiotics. All cultures were maintained at 37°C in 5% CO 2 / 95% air humidified air. Med...

Embodiment 2

[0048] Treatment of cell cultures with ascorbic acid and / or calcium threonate

[0049] In the presence of freshly prepared supplements consisting of increasing concentrations of ascorbic acid (AA) or calcium ascorbate (CA), 0.25-1.0 x 10 5 Tumor-derived cells or cells of normal hepatic cell lines were seeded and cultured in individual wells of 24-well plates. Cultures were periodically supplemented with the respective supplements, with or without media changes as indicated. Controls consisted of cells that received growth medium without added supplements.

[0050] For calcium threonate treatment, cells were allowed to attach by overnight incubation. The next day, threonate treatment was started using one of two protocols.

[0051] In one protocol (short-term exposure), monolayers were washed and exposed directly to 1 ml / well of 7.5-30 mM threonate (in Ringer's solution) for a short period at 37°C as described by Fay and Verlangieri (above for reference). Controls ...

Embodiment 3

[0054] Cell culture treated with ascorbic acid plus calcium threonate

[0055] As in Example 2, cells were treated with calcium threonate (CT) at 37°C for 60 minutes, followed by the addition of ascorbic acid (AA) in Ringer's solution and continuous incubation for 30 minutes. At the end of the treatment period, the solution was removed and replaced with 1 ml / well of growth medium.

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PUM

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Abstract

A selective chemotherapy method incldues the step of contacting tumor cells with a mineral ascorbate / vitamin C metabolite composition. A chemotherapeutic composition comprises the mineral ascorbate / vitamin C metabolite composition in a pharmacologically acceptable intravenous carrier.

Description

field of invention [0001] The present invention relates to a tumor cytotoxic chemotherapy method. [0002] In another aspect the invention relates to tumor cytotoxic chemotherapeutic compositions. [0003] More uniquely, the present invention relates to tumor cytotoxic chemotherapy methods and compositions for the treatment of cancer in a human host Background of the invention [0004] Tumor cytotoxic chemotherapeutic agents preferentially cause the death of malignant cells (apoptosis). Because of the similarities between normal and malignant cells (both originate from the same host), a chemotherapy dose that induces apoptosis in tumor cells can also be toxic to normal cells. Tumortoxic agents must often raise acceptable side-effect limits for re-administration. Ideally, tumor cytotoxic agents should be "selective", that is, at lower doses required to cause tumor cell death to be effective as a tumor cytotoxic chemotherapeutic agent versus higher doses that are toxic to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/34A61K31/341A61K31/35A61K31/351A61K31/375A61K31/47A61K31/555A61K31/7004A61P35/00
CPCA61K31/7004A61K31/47A61K31/555A61K31/35A61K31/375A61K31/34A61P35/00A61K2300/00
Inventor R·J·贾里瓦拉
Owner 易思特C公司
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