Two-stage citric acid fermentation process with whole wheat flour as raw material

Abstract

The two stage fermentation technology of citric acid using whole wheat flour as raw material is characterized in that whole wheat flour is directly mixed with water in wt. ratio of 1:5-1:8 to prepare paste, raise temp. to 85 deg.c, adding high temp. amylase, raising paste temp. to 90 deg.C until completely liquified; quick filtration to obtain liquified liquid, fill liquified liquid into fermentation tank, heat and sterilized, than quickly lower temp. to 35 deg.C, inocculate first stage culture into fermentation tank and ferment until fermentation is complete. Advantages include: simple and ratioal technology, low cost on raw material, equipment and energy consumption, can raise saccharification rate of row material and fermentation conversion rate etc.

Description

technology field;

[0001] The present invention relates to the preparation method of citric acid. More specifically, the secondary fermentation process in the production of citric acid. Background technique:

[0002] Citric acid is a highly acidic organic acid, easily soluble in water, alcohol, and insoluble in organic solvents. Citric acid has an extremely wide range of uses in many industries. Including the sour flavoring agent used in the food industry as a drink, the raw material of many medicines in the pharmaceutical industry, and the excellent polyvalent mixture in the chemical industry. Citric acid is a relatively important short-term product among organic acids. The growth rate of world consumption has always been maintained at 7% to 10%, and its use is still expanding.

[0003] The preparation process of citric acid includes: culture of strains—introduction of strains, preparation of primary culture through primary fermentation—incorporation of primary culture fo...

Examples

Embodiment 1

[0024] 1. Liquefaction. According to the capacity of the liquefaction tank, the whole wheat flour and water are slurried at a weight ratio of 1:5; then heated in the liquefaction tank, and when the temperature rises to 85°C, high-temperature amylase is added at a time, and the amount added is 1 gram of whole wheat flour. 4 units of high temperature amylase. Continue to heat up to 90°C until completely liquefied.

[0025] 2. Filter. The above-mentioned liquefied liquid contains liquefied liquid and wheat dregs, which are separated by a centrifuge, and the separated liquefied liquid is pumped into a fermenter through a pipeline.

[0026] 3. Heat and disinfect. When the temperature reached 85°C, the heating was stopped, and the temperature was rapidly lowered, and the temperature was lowered to 35°C, and the primary culture was transferred into the fermenter until the fermentation was completed.

[0027] The extraction of citric acid can adopt the current general calcium salt...

Embodiment 2

[0029] 1. Liquefaction. According to the capacity of the liquefaction tank, the whole wheat flour and water are mixed according to the weight ratio of 1:6; then heated in the liquefaction tank, and when the temperature rises to 85°C, high-temperature amylase is added at a time, and the amount added is 1 gram of whole wheat flour Add 6 units of high-temperature amylase, and continue to heat up to 90°C to maintain complete liquefaction.

[0030] 2. Filter. The above-mentioned liquefied liquid contains liquefied liquid and wheat dregs, which are separated by bag-type vacuum filtration, and the separated liquefied liquid is pumped into a fermenter through a pipeline.

[0031] 3. Heat and disinfect. When the temperature reached 85°C, the heating was stopped, and the temperature was rapidly lowered, and the temperature was lowered to 35°C, and the primary culture was transferred into the fermenter until the fermentation was completed.

[0032] The extraction of citric acid can ad...

Embodiment 3

[0034] 1. Liquefaction. According to the capacity of the liquefaction tank, the whole wheat flour and water are mixed according to the weight ratio of 1:8; then they are heated in the liquefaction tank, and when the temperature rises to 85°C, high-temperature amylase is added at one time, and the amount added is 1 gram of whole wheat flour Add 8 units of high-temperature amylase, and continue to heat up to 90°C to maintain complete liquefaction.

[0035] 2. Filter. The above-mentioned liquefied liquid contains liquefied liquid and wheat dregs, which are separated by a plate and frame filter press method, and the separated liquefied liquid is pumped into a fermenter through a pipeline.

[0036] 3. Heat and disinfect. When the temperature reached 85°C, the heating was stopped, and the temperature was rapidly lowered, and the temperature was lowered to 35°C, and the primary culture was transferred into the fermenter until the fermentation was completed.

[0037] The extraction...

technology field;

[0001] The present invention relates to the preparation method of citric acid. More specifically, the secondary fermentation process in the production of citric acid. Background technique:

[0002] Citric acid is a highly acidic organic acid, easily soluble in water, alcohol, and insoluble in organic solvents. Citric acid has an extremely wide range of uses in many industries. Including the sour flavoring agent used in the food industry as a drink, the raw material of many medicines in the pharmaceutical industry, and the excellent polyvalent mixture in the chemical industry. Citric acid is a relatively important short-term product among organic acids. The growth rate of world consumption has always been maintained at 7% to 10%, and its use is still expanding.

[0003] The preparation process of citric acid includes: culture of strains—introduction of strains, preparation of primary culture through primary fermentation—incorporation of primary culture fo...

Examples

Embodiment 1

[0024] 1. Liquefaction. According to the capacity of the liquefaction tank, the whole wheat flour and water are slurried at a weight ratio of 1:5; then heated in the liquefaction tank, and when the temperature rises to 85°C, high-temperature amylase is added at a time, and the amount added is 1 gram of whole wheat flour. 4 units of high temperature amylase. Continue to heat up to 90°C until completely liquefied.

[0025] 2. Filter. The above-mentioned liquefied liquid contains liquefied liquid and wheat dregs, which are separated by a centrifuge, and the separated liquefied liquid is pumped into a fermenter through a pipeline.

[0026] 3. Heat and disinfect. When the temperature reached 85°C, the heating was stopped, and the temperature was rapidly lowered, and the temperature was lowered to 35°C, and the primary culture was transferred into the fermenter until the fermentation was completed.

[0027] The extraction of citric acid can adopt the current general calcium salt...

Embodiment 2

[0029] 1. Liquefaction. According to the capacity of the liquefaction tank, the whole wheat flour and water are mixed according to the weight ratio of 1:6; then heated in the liquefaction tank, and when the temperature rises to 85°C, high-temperature amylase is added at a time, and the amount added is 1 gram of whole wheat flour Add 6 units of high-temperature amylase, and continue to heat up to 90°C to maintain complete liquefaction.

[0030] 2. Filter. The above-mentioned liquefied liquid contains liquefied liquid and wheat dregs, which are separated by bag-type vacuum filtration, and the separated liquefied liquid is pumped into a fermenter through a pipeline.

[0031] 3. Heat and disinfect. When the temperature reached 85°C, the heating was stopped, and the temperature was rapidly lowered, and the temperature was lowered to 35°C, and the primary culture was transferred into the fermenter until the fermentation was completed.

[0032] The extraction of citric acid can ad...

Embodiment 3

[0034] 1. Liquefaction. According to the capacity of the liquefaction tank, the whole wheat flour and water are mixed according to the weight ratio of 1:8; then they are heated in the liquefaction tank, and when the temperature rises to 85°C, high-temperature amylase is added at one time, and the amount added is 1 gram of whole wheat flour Add 8 units of high-temperature amylase, and continue to heat up to 90°C to maintain complete liquefaction.

[0035] 2. Filter. The above-mentioned liquefied liquid contains liquefied liquid and wheat dregs, which are separated by a plate and frame filter press method, and the separated liquefied liquid is pumped into a fermenter through a pipeline.

[0036] 3. Heat and disinfect. When the temperature reached 85°C, the heating was stopped, and the temperature was rapidly lowered, and the temperature was lowered to 35°C, and the primary culture was transferred into the fermenter until the fermentation was completed.

[0037] The extraction...