Method of preparing multi tissue paraffin embedded wax lump of tissue chip and its device

Abstract

The temperature of the melted paraffin wax is controlled at the temperature higher than its melting point about 5-10 deg.C by use of the thermostatic water bath box with the switch for controlling temperature. The thermostatic water bath under the embedding through controls the embedding temperature at the range of the melting point of paraffin wax. Since the pump with negative pressure is connected to the negative pressure box located below the embedding trough, the negative pressure is formed at the bottom part. Thus, with the melted paraffin wax being added in, the air between the tissue sample and the thin wax block is sucked out, and the wax is filled in the gap so as to form the integrated body. The multiple tissues with wax wrapped with high quality can be prepared quickly by usingthe invented device and method.

Description

1. Technical field [0001] The invention belongs to the manufacture of a tissue chip and a method thereof in the medical field, in particular to a method for making a multi-tissue paraffin-embedded wax block that can be used for preparing a tissue chip and a device for realizing the method. The method and device of the invention can be widely used in the preparation process of tissue chips. 2. Background technology [0002] In the process of preparing tissue chips, it is first necessary to make multi-tissue paraffin-embedded wax blocks, and the quality of multi-tissue paraffin-embedded wax blocks is related to the quality of tissue chips. When preparing tissue chips, how to ensure the accurate positioning of multi-tissue samples Embedding and removing the air between the tissue sample and the wax block embryo, so that the tissue sample and the thin layer of wax block form a complete whole is a difficult problem for multi-tissue embedding. This difficulty increases with the n...

AI Technical Summary

Problems solved by technology

This difficulty increases with the number of embedded tissue samples

[0003] According to the current existing technology, when the thin-layer wax block with multi-tissue samples is directly put into the molten paraffin, because the solidified thin-layer wax block is relatively light, it is difficult to sink into the bottom of the embedding frame smoothly, or it cannot be used. It is parallel to the bottom of the embedding frame, so that the tissue samples are on the same plane. Moreover, if the temperature of the molten paraffin is too high, the thin layer of paraffin embryos will be melted, and the tissue samples arranged in it will be convenient due to their low density. Floating and moving can not achieve the purpose of positioning and embedding; if the temperature is too low, the melted paraffin will form a paraffin shell outside the thin layer of paraffin

In addition, if the air between the tissue sample and the thin layer of paraffin cannot be effectively excluded, the tissue sample and the thin layer of wax block embryo cannot form a complete whole, resulting in incomplete sections

[0004] According to the existing technology at present, when directly adding the melted paraffin on the thin layer wax block with multi-tissue samples, the same reason, if the temperature of the melted paraffin is too high, the thin layer paraffin embryo will be melted, The tissue samples arranged in it will float and move due to their low density, which cannot achieve the purpose of positioning and embedding. If the temperature is too low, the melted paraffin will form a layer of paraffin shell outside the thin layer of paraffin. In addition, the tissue The air between the sample and the thin layer of paraffin cannot be excluded, and the tissue sample and the thin layer of wax block embryo cannot form a complete whole, resulting in incomplete sections

[0005] It can be seen that the current existing technology cannot successfully complete the rapid preparation of multi-tissue paraffin-embedded wax blocks, and ensure that the tissue samples in the prepared wax block are at the same level, and the positioning is accurate and closely combined with the wax block

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