Turnip heat tolerance character screening process
A technology for heat-resistant traits and screening methods, applied in the directions of botanical equipment and methods, applications, and plant phenotype improvement, can solve problems such as loss of excellent germplasm, and achieve the effects of shortening breeding years, being easy to master, and improving effectiveness
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[0019] The technical solution of the present invention will be further described below through specific examples.
[0020] The tested varieties are: Xiaqiu radishes: Shangnong 936, Shangxuan No. 4, Duanye 13, Zhongqiuhong and Liushiri; autumn and winter radishes: Chuanxinhong and Shaoxindianhong; spring radishes: Baiyuchun.
[0021] ①Determination of high temperature half-lethal time
[0022] The seeds of the above 8 parts of materials were sterilized with saturated bleaching powder solution for 15-20 minutes, and sowed in a seedling tray equipped with a substrate (the composition of the substrate: 60% peat+20% perlite+20% vermiculite), and the seedlings were grown The trays were placed in a growth chamber with a temperature controlled at 25°C / 18°C (day / night), during which nutrient solutions (1 / 10 MS macroelements and trace elements) were supplemented 4 times a week.
[0023] Take 30-day-old leaves, use a hole puncher with a hole diameter of 0.5cm to punch small discs, rinse...
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