Tissue culture reproduction method of snake grass

A technology for tissue culture and heliconia, applied in the field of culture medium, can solve problems such as no large-scale production and patent application, and achieve the effects of strong resistance, rapid emergence and high stability

Inactive Publication Date: 2003-09-17
SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are only a few preliminary experimental studies on tissue culture of a small number ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Material: Rhizome of Heliconia rostrata Ruiz & Pavon.

[0016] 2. The method of explant induction material disinfection: excavate vigorously growing heliconia rhizomes from the field, remove the roots and leaves, rinse with tap water, and soak in 0.1% potassium permanganate solution for 5 minutes , take out the towel and dry the rhizomes, then soak them in 0.1% carbendazim solution for 5 minutes, pack them in a black plastic bag with a small amount of medicine, and place them in an incubator at about 30°C for dark cultivation. A 4-6 centimeter sprout can be formed, and the sprout is used as an explant for preliminary sterilization. Soak the explants in alcohol for 30 seconds on the ultra-clean workbench, then soak them in 0.1% mercury liter for 10 minutes, rinse them with sterile water for 3 times, and peel off the scales of the buds with a scalpel. For each layer of scales, use 0.1 Soak in % mercury chloride for 1 minute, rinse with sterile water three times until ...

Embodiment 2

[0023] Rainbow heliconia (H. psittacorum L.f.cv. Sassy) was used as the explant. The basic operation method is consistent with embodiment 1. But its adventitious bud induction medium is MS+6-benzylpurine 5 mg / L+naphthalene acetic acid 0.5 mg / L+15% coconut water; cluster bud proliferation medium is MS+6-benzylpurine 5 mg / L+ Naphthalene acetic acid 0.5 mg / L; strong seedling medium is MS+6-benzyl purine 1 mg / L + naphthalene acetic acid 0.1 mg / L. The survival rate of transplanted test-tube seedlings can reach 100%.

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Abstract

A tissue culture method for reproducing the scorpion-tail canna which has high appreciative value due to its shape and gay colours of its flower, its culture media and reproduction method, and the disinfecting method for its explant are disclosed. Its advantages are high speed and high effect.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of Heliconia heliconia and the culture medium used. technical background [0002] Heliconia originally belonged to the genus Heliconia in the Strelitziaceae family, and now most scholars regard it as an independent Heliconiaceae. Heliconia is a perennial herb. There are more than 80 native species of this genus, and more than 400 varieties, horticultural cultivars, and hybrids. They are mainly distributed in tropical America and the Pacific Islands. Many of them have inflorescences with Bright colors and unique shapes are flowers with high ornamental value. They are high-grade greening trees for roads, parks and courtyards. They can be planted in clusters, columns, or single plants; the dwarf Heliconia can also be planted in pots Some species, because of their strong shade tolerance, can also be used as potted plants indoors; at the same time, because of their bright colors and long ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曾宋君吴坤林张奕奇段俊
Owner SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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