Method for checking pesticide residue and portable detecting instrument thereof

A portable technology for pesticide residue detection, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as inability to meet the requirements of rapid analysis, and achieve the effects of small size, simple instrument structure, and convenient use

Inactive Publication Date: 2004-11-10
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

However, due to the need to use a constant temperature device and 220V power supply, it still cannot meet the requirements of on-site and field rapid analysis

Method used

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  • Method for checking pesticide residue and portable detecting instrument thereof
  • Method for checking pesticide residue and portable detecting instrument thereof
  • Method for checking pesticide residue and portable detecting instrument thereof

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Embodiment Construction

[0026] In enzymatic assays, the absorbance value A increases continuously over time t. By measuring the slope K value of the absorbance-time curve (abbreviated as A-t curve) of the sample, the inhibition rate Y can be calculated:

[0027] Y=K t / K 0 ×100%

[0028] In the formula: K t is the slope value of the A~t curve of the sample to be tested at temperature T°C, K 0 is the slope value of the A~t curve of the blank control sample (abbreviated as blank sample) without pesticide, and Y is the inhibition rate. The magnitude of the inhibition rate reflects the magnitude of the toxicity, Y=0% when completely non-toxic, the more toxic the sample, the higher the Y value. We can judge the toxicity of the residual pesticides on the sample according to the Y value. It is generally believed that the sample is non-toxic when Y50%, it is determined that the pesticide residues in the sample exceed the standard and are not edible.

[0029] When traditional instrum...

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Abstract

The invention discloses a pesticide residue detecting method and its portable detector. It omits the step of correcting instrumental light source I0 and reduces measuring error source; advances a temperature correcting model, omits 37 deg.C constant-temperature measuring condition, and needs no more heavy constant-temperature devices like water bath tank, etc. The detector: under the control of light source voltage stabilizing control circuit, the monochromatic light source generates monochromatic light; the monochromatic light beam passes through colorimetric tank holding the solution inside to be detected to irradiate a photoelectric sensor; the sensor converts the detected light intensity value to a voltage value, and then transmits the voltage to an A/D converter to convert into a digital signal, then the digital signal is inputted in a MCU system for data processing, a temperature measuring circuit converts the detected temperature signal to a digital signal to send into the MCU for temperature correction. It is especially suitable for the requirement of field fast screening or semiquantitative determination. It has simple structure, small bulk, and convenient use and can directly indicate the toxicity of pesticide residue.

Description

technical field [0001] The invention relates to a pesticide detection technology, which is a method suitable for rapidly detecting the toxicity of organophosphorus and carbamate pesticides on site and a portable detection instrument thereof. technical background [0002] my country is a large agricultural country, and there is a great demand for pesticides. The wide application of pesticides has caused serious pollution to the environment. Due to the danger caused by pesticide pollution to human beings, developed countries implement the detection of pesticide residues before the sale of fruits and vegetables, and the export of agricultural and sideline products in my country requires the detection of pesticide residues. The implementation of pollution-free vegetables and food safety plans also requires corresponding on-site rapid detection technology. [0003] At present, the quantitative analysis of pesticide residues generally adopts chromatographic analysis, using labora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/05
Inventor 陆晓华陈光东杨海金伟段三丁
Owner HUAZHONG UNIV OF SCI & TECH
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