Engineering strain of Brasil diazotrophic spirillum DraT* containing multiple copied nifA gene

A Brazilian Azospirillum, engineering strain technology, applied in bacteria, organic fertilizers and other directions

Inactive Publication Date: 2004-12-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the field test of Azospirillum brasiliensis ammonium-resistant engineered bacteria in foreign literature and foreign patent searches, and there is no report of field inoculation with Azospirillum brasiliensis ammonium-resistant engineered bacteria

Method used

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  • Engineering strain of Brasil diazotrophic spirillum DraT* containing multiple copied nifA gene

Examples

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Embodiment Construction

[0017] The present invention will be further described through construction examples and application examples of the engineering strain YuMA below.

[0018] 1. Construction of engineering strain YuMA

[0019] (1) Azospirillum brasiliensis DraT - Mutant strain construction

[0020] In the present invention, the total chromosomal DNA is first extracted from the genome library of Azospirillum brasiliensis Yu62, then digested with Sau3A, and a DNA fragment of 8-20 kb is recovered, connected to the phage carrier EMBL3A (Huamei Bioengineering Co., Ltd.) and packaged in vitro. A gene library of Azospirillum brasiliensis Yu62 was constructed. Part of the draT gene fragment of the Azospirillum brasiliensis Sp7 strain was used as a probe to screen a positive clone from the Azospirillum brasiliensis Yu62 gene library, and the length of the positive clone was 8.0 kb. The positive clone was digested with SalI, and the 8.0 kb foreign fragment containing the draT gene was recovered and clon...

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PUM

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Abstract

An Azospirillum brasilense DraT-engineering strain containing multi-copied nifA gene is disclosed, with preserving number of CGMCC No.0876. This bacterial manure containing engineering strain of Azospirillum brasilense DraT- with multi-copied nifA is also disclosed. The cultured engineering strain as bacterial manure is applied for field plant of corn and wheat to decrease use amount of urea, increase yield and save manure, promote plant growth by IAA.

Description

technical field [0001] The invention relates to an engineering bacterium of Azospirillum brasiliensis, and also relates to a bacterial fertilizer. Background technique [0002] The improvement of the output of most crops such as wheat, corn and rice mainly depends on the increase of the application of chemical nitrogen fertilizers. The unrestrained application of large amounts of chemical fertilizers with the sole goal of increasing the output will result in soil compaction, decreased fertility, and the environment especially Serious water pollution and other issues. [0003] Biological nitrogen fixation is an important way to reduce the use of nitrogen fertilizers, alleviate environmental pollution, and reduce agricultural production costs. According to the relationship between nitrogen-fixing microorganisms and host plants, nitrogen fixation can be divided into autogenous nitrogen fixation, joint nitrogen fixation and symbiotic nitrogen fixation (Edited by You Chongpyo, B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12N1/21
Inventor 李季伦陈三凤张耀平赵银锁马旅雁王娟
Owner CHINA AGRI UNIV
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