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Engineering strain of Brasil diazotrophic spirillum DraT* containing multiple copied nifA gene

A Brazilian Azospirillum, engineering strain technology, applied in bacteria, organic fertilizers and other directions

Inactive Publication Date: 2006-05-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the field test of Azospirillum brasiliensis ammonium-resistant engineered bacteria in foreign literature and foreign patent searches, and there is no report of field inoculation with Azospirillum brasiliensis ammonium-resistant engineered bacteria

Method used

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  • Engineering strain of Brasil diazotrophic spirillum DraT* containing multiple copied nifA gene

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Effect test

Embodiment Construction

[0017] Hereinafter, the present invention will be further illustrated through construction examples and application examples of the engineering strain YuMA.

[0018] 1. Construction of engineering strain YuMA

[0019] (1) Brazilian Azospirillum DraT - Construction of mutant strains

[0020] The present invention first extracts total chromosomal DNA from the genomic library of Azospirillum brasiliensis Yu62, then digests with Sau3A, recovers 8-20 kb DNA fragments, connects to the phage vector EMBL3A (Huamei Bioengineering Company) and packs in vitro, A gene library of Azospirillum brasiliensis Yu62 was constructed. A positive clone was screened from the Yu62 gene library of Azospirillium brasilianum Sp7 strain as a probe, and the length of the positive clone was 8.0kb. The positive clones were digested with SalI, and the 8.0 kb foreign fragment containing the draT gene was recovered and cloned into pUC19 (purchased from Huamei Bioengineering Company) to obtain the recombinant plasmid...

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Abstract

An Azospirillum brasilense DraT-engineering strain containing multi-copied nifA gene is disclosed, with preserving number of CGMCC No.0876. This bacterial manure containing engineering strain of Azospirillum brasilense DraT- with multi-copied nifA is also disclosed. The cultured engineering strain as bacterial manure is applied for field plant of corn and wheat to decrease use amount of urea, increase yield and save manure, promote plant growth by IAA.

Description

Technical field [0001] The present invention relates to an engineered strain of Azospirillum brasiliensis, and also relates to a bacterial fertilizer. Background technique [0002] The increase in the yield of most crops such as wheat, corn and rice mainly depends on the increase in the application of chemical nitrogen fertilizers. The uncontrolled application of large quantities of chemical fertilizers with the sole goal of increasing yields results in soil compaction and reduced fertility, especially the environment. Serious water pollution and other problems. [0003] Biological nitrogen fixation is an important way to reduce the use of nitrogen fertilizers, alleviate environmental pollution, and reduce agricultural production costs. According to the relationship between nitrogen-fixing microorganisms and host plants, nitrogen fixation is divided into autogenous nitrogen fixation, combined nitrogen fixation and symbiotic nitrogen fixation (Editor-in-Chief You Chongpiao, Biolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C05F11/08
Inventor 李季伦陈三凤张耀平赵银锁马旅雁王娟
Owner CHINA AGRI UNIV
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