37results about How to "Improve nitrogen fixation efficiency" patented technology

The invention discloses a phosphorus transportprotein gene GmPT5 related to phosphorus transport of soybean nodulation and application thereof. The phosphorus transportprotein gene GmPT5 related to the phosphorus transport of the soybean nodulation has a nucleotide sequence as shown in SEQ ID NO:1, and coded protein nucleotide sequence is shown as SEQ ID NO:2. The gene GmPT5 regulates and controls the phosphorus transport from a root part to the nodulation, furthermore, the overexpression of the gene is favorable for increasing the size of the soybean nodulation and the plant biomass as well as the nitrogen and phosphorus contents.

An Azospirillum brasilense DraT-engineering strain containing multi-copied nifA gene is disclosed, with preserving number of CGMCC No.0876. This bacterial manure containing engineering strain of Azospirillum brasilense DraT- with multi-copied nifA is also disclosed. The cultured engineering strain as bacterial manure is applied for field plant of corn and wheat to decrease use amount of urea, increase yield and save manure, promote plant growth by IAA.

A method for planting the plant on the vertical rock wall in quarry includes preparing slope surface, arranging anchor rods on the slope surface, arranging the plant growing lattice, arranging composite nutritive bags and nutritive bowls, spreading net on the slope surface, sowing the seeds of proper bush, tree, grass and vine plant, and spray irrigation.

The invention discloses a preparing method of a surface-defect-mode-modified metal sulfide catalyst. The preparing method comprises the following steps of 1, weighing nitrate, hydrated indium nitrateor hydrated lanthanum nitrate or hydrated zinc nitrate, and cysteine respectively, putting the raw materials in a beaker containing deionized water, and conducting stirring and dissolving to obtain amixed solution; 2, transferring the mixed solution in step 1 into a polytetrafluoroethylene lining, conducting sealing and hydro-thermal treatment, afterwards, utilizing deionized water for washing, and then conducting vacuum drying to obtain the surface-defect-mode-modified metal sulfide catalyst. According to the catalyst, the N2 molecular reaction activity is improved, and proceeding of a nitrogen fixation reaction is promoted. The preparing method is simple and feasible in synthesis route and has universality.

The invention discloses a rectangular ingot for an anti-corrosion mold and a process method of the rectangular ingot, and belongs to the technical field of metallurgy production processes. The rectangular ingot for the anti-corrosion mold is high in strength, and the corrosion resistance, abrasion resistance, creep resistance and fatigue resistance are obviously improved. The process method for producing the rectangular ingot for the anti-corrosion mold is further provided. The rectangular ingot is prepared from, by weight part, 0.25%-0.65% of C, 0.4%-0.8% of Si, 0.4%-0.9% of Mn, 12%-18% of Cr, 0.05%-0.3% of Ni, 0.1%-0.3% of Mo, 0.06%-0.30% of N, not larger than 0.02% of Al, not larger than 0.020% of P, not larger than 0.005% of S and the balance Fe and inevitable impurity elements. The process method comprises the steps of smelting of molten steel with the nitrogen content smaller than or equal to 45 ppm, nitrogen-rich manganese-rich molten steel refining and die casting molding, wherein nitrogen-rich manganese-rich molten steel refining comprises large-slag-amount refining carried out in a refining furnace and secondary refining carried out in a vacuum refining furnace.

The invention discloses a selenium-rich and chromium-rich peanut culture formulated fertilizer, prepared from the following raw materials in parts by weight: 400-600 parts of urea, 100-200 parts of ammonium sulfate, 250-300 parts of monoammonium phosphate, 100-150 parts of potassium chloride, 20-50 parts of humic acid, 5-10 parts of compound trace elements, 40-60 parts of bentonite, 1-2 parts of inorganic selenium salt and 0.01-0.05 part of chromium trichloride. An application method of the selenium-rich and chromium-rich peanut culture formulated fertilizer comprises the steps of selecting clay land with a deep soil layer and without fertilizer leakage, and performing basal application in one step by taking brown coal and turf as carriers, and separating seeds from the fertilizer. According to the compound fertilizer and the application method, the advantages of high peanut yield, strong plant disease resistance and suitability for large-area application are achieved, fruited peanut ears are bigger and heavy, and good in quality, and selenium-rich and chromium-rich peanuts contain a selenium element and a trivalent chromium element necessary for human bodies and animals, and are high in nutrition value and beneficial to health.

The invention discloses a method and system for preparing a green nitrogen fertilizer by adopting plasmas. The method comprises the steps that collected energy is distributed and used for driving loaded energy to generate the plasmas through boosting, and the plasmas discharge electricity to generate oxynitrides; the oxynitrides are subjected to compression dissolution in absorption liquid to generate the nitrogen fertilizer. The system comprises an energy collecting device, an energy control device, an energy storage device, a boosting device and a nitrogen fertilizer generating device, and the nitrogen fertilizer generating device comprises a plasma generating module, a compression module and an absorption module. According to the method, renewable energy is taken as the drive by combining energy storage and control, compressed air is adopted for fully dissolving NOx generated from discharging of the plasmas, safe operation is ensured, and the nitrogen fixing efficiency is improved.

The invention discloses a biological nitrogen fixation synergist. The biological nitrogen fixation synergist is prepared from in percentage by weight: 0.05-5% of charcoal, 0.01-5% of graphene sol, 0.01-1% of iron nutrient, 0.01-1% of molybdenum nutrient, 0.00001-0.0005% of brassinolide active substance, 0.1-1% of surfactant and the balance stopping. The biological nitrogen fixation synergist provides rich uncombined carbon, can promote colonization of nitrogen-fixing microorganisms, improves the dinitrogenase activity, promotes nitrogen absorption and utilization of plants, and improves the yield. The invention further provides a use method of the biological nitrogen fixation synergist.

The invention relates to a special slow release water-soluble fertilizer for soybeans, and a preparation method thereof. Raw material components of the special slow release water-soluble fertilizer for soybeans comprise 140-150 parts of a urea formaldehyde fertilizer, 40-50 parts of diammonium phosphate, 60-70 parts of potassium dihydrogen phosphate, 50-60 parts of potassium nitrate, 10-15 parts of a chelated medium and trace element premix, 3-4 parts of sodium molybdate, 10-15 parts of soluble organic matters, 4-5 parts of a solubilizer and 3-5 parts of an amide hydrogel material. The invention also relates to the preparation method of the special water-soluble fertilizer for soybeans. Parts of nitrogen and phosphorus nutrients of the special slow release water-soluble fertilizer for soybeans have a slow release effect, and the special slow release water-soluble fertilizer for soybeans also contains a small amount of soluble organic matters, has comprehensive nutrition, meets soybean demands, accords with the nutrient absorption rule of a soybean generation period, can increase the yield of soybeans and improve the quality of the soybeans, and has improved fertilizer effect utilization rate.

A high-efficient nitrogen-fixing engineering bacterium, its construction and use are disclosed. The engineering bacterium is prepared by inducing copied plasmid containing DctBDP fragment with tetrodicarboxylic acid trans-gene into combined nitrogen-fixing engineering bacterium and obtaining high-efficient nitrogen-fixing engineering bacterium. It can be used for high-efficient nitrogen-fixing bacterial manure.

The invention belongs to the field of plant cultivation, and particularly relates to a mud flat soil improvement method. The method has a remarkable saline-alkali soil improvement effect. Through crop rotation of broad beans and sesbania, saline-alkali soil improvement can be rapidly completed, the broad beans in the improvement period can be used as green manure after agricultural products are harvested, the sesbania can be cut multiple times to be used as green manure and livestock feed, the saline-alkali soil improvement effect is remarkable, grain crops such as wheat can be normally planted in the improved saline-alkali soil, and the saline-alkali soil becomes a grain field; the method has good economic and social benefits.

The invention discloses a HD-Zip I type transcription factor GmHDL 57 gene with ability of improving salt resistance of leguminous plants. The sequence of the gene is a nucleotide sequence shown as SEQ ID NO:1, and a coded protein sequence is an amino acid sequence shown as SEQ ID NO: 2. The invention further discloses application of the HD-Zip I type transcription factor GmHDL 57 gene with ability of improving salt resistance of leguminous plants in lotus corniculatus. By utilizing an overexpression technique, the salt resistance of the gene in the leguminous plant lotus corniculatus is researched. Results show that compared with control plants, the salt resistance of the plants is obviously enhanced after overexpression, and the gene has great application prospects for widening a planting range of the leguminous plants in saline-alkali soil.

The invention relates to a specific promoter in a leguminous plant legume related tissue and application thereof. The promoter is particularly suitable for driving the specific expression of exogenous genes in the leguminous plant legume related tissue, such as legumes or seed leaves, and the expression level of the exogenous genes in other tissues of leguminous plants is extremely low or the exogenous genes are not expressed in the other tissues of the leguminous plants. The expression specificity and specificity of the promoter are very high. The invention further provides constructions, expression kits, carriers and the like with the promoter. For transgenic leguminous plants containing the promoter disclosed by the invention, agronomic traits of the leguminous plants can be specifically improved, so that the influence on the other tissues of the leguminous plants caused by the introduction of the exogenous genes is effectively avoided, the exogenous genes can be specifically expressed in the legume related tissue, and thus, various related products are obtained.

The invention relates to a method to promote rapid increase of organic matters in tidal flat virgin soil by Sesbania cannabina mowing mode. In order to solve the problems that costal newly-enclosed tidal flat soil of Jiangsu lacks organic matters, salts are easily returned, and it is difficult for common crops to grow, the invention provides the method to promote rapid increase of organic mattersin tidal flat virgin soil by Sesbania cannabina mowing mode. The method includes: applying 1000 kg / 667 m<2> of organic fertilizer made by fermenting cow dung, chicken dung, rice chaff and the like totidal flat virgin soil, deeply tilling by machinery, applying suitable calcium superphosphate, and performing mechanical rotary tillage; building small ridges 10-15 cm in height around a field, and burying drain pipes communicated with outside ditches; ditching to sow seeds in mid-June, mowing Sesbania cannabina once at intervals of about 45 days, with stubbles about 55 cm in height left, spreading the mowed parts evenly among the rows, and topdressing with nitrogen fertilizer in time; when mowed stubbles grow to the full-bloom stage of Sesbania cannabina, and crushing and rolling in time.

The invention discloses a soil improvement method of an ecological tea garden and relates to the technical field of ecological tea garden establishment. The soil improvement method is characterized by including the steps of 1), land selection; 2), land regulation; 3), planting of leguminous green manure; 4), application of weathering coal; 5), application of organic fertilizer containing rhizobium; 6), adjustment of soil aid and alkali in the tea garden. The soil improvement method has the advantages that soil is well improved via placement and cultivation application of the weathering coal and planting of the leguminous green manure, the soil is fertile, fertility is long and stable, the soil is loosen, construction of the standardized ecological tea garden and development of tea industry are benefited, and higher market demand is met.

The invention provides a soil nitrogen fixation moistening agent which comprises the following raw materials in parts by weight: 10-300 parts of palygorskite, 10-300 parts of silicon powder, 10-300 parts of magnesium sulfate, 10-200 parts of boric acid, 10-100 parts of molybdenum ammonium acid, 10-100 parts of glucose, 1-200 parts of rare earth and 100-200 parts of nodule bacterium powder. By adopting the soil nitrogen fixation moistening agent provided by the invention, on one hand, medium and trace elements essential for crops to grow can be comprehensively replenished, on the other hand, the use amounts of nitrogen fertilizers and phosphate and potash fertilizers can be greatly reduced, and finally due to addition of biological nitrogen fixation nodule bacterium powder, the soil nitrogen fixation moistening agent is relatively high in nitrogen fixation efficiency and relatively effective in environment protection.

The invention relates to the field of liquid fertilizer, in particular to liquid fertilizer for water culture of cucumber. The liquid fertilizer is prepared from raw materials as follows: sodium acetate, sodium carbonate, sodium thiosulfate, sodium chloride, magnesium sulfate, urea phosphate, potassium chloride, yeast extract, water and photosynthetic bacterial suspensions. The liquid fertilizer has the advantages that the selected strain is high in purity, the microecology of effective viable bacteria is stable, the bacterial metabolite is exuberant and the like, and can significantly increase the growth rate of the cucumber, increase the seedling biomass, promote the development of young roots, reduce the diseases, effectively decompose allelo-chemicals secreted by cucumber roots and increase the yield.

The invention discloses an in-situ efficient compound rhizobium flora, and belongs to the technical field of microorganisms. The in-situ efficient compound rhizobium flora is prepared by the following method: selecting fresh soybean root nodules which grow well, directly putting the fresh soybean root nodules into 200ml of YMA liquid culture medium without disinfection or crushing, culturing for 5 days at 28 + / -2 DEG C, then sucking 1ml of bacterial liquid, adding the bacterial liquid into 200ml of YMA liquid culture medium for subculture, generating a generation every 5 days, and performing passage for 50 days until the pH is stable to obtain the stable in-situ efficient compound rhizobium flora. The in-situ efficient compound rhizobium flora has the advantages of improving the soybean nodulation efficiency, improving the soybean nitrogen fixation efficiency, reducing the application of chemical fertilizers and pesticides, and having good ecological benefits and wide application prospects.

The invention discloses a bidirectional electrocatalytic nitrogen fixation device and method capable of simultaneously generating nitric acid and ammonia, NOx gas is reduced into NH3 at a cathode and oxidized into NO3 <-> at an anode based on an improved H-type electrolytic tank, and efficient harmless treatment and high-value utilization of harmful waste gas NOx are realized. A working electrode catalyst in an electrolysis system is prepared by simultaneously utilizing physical deposition and chemical growth methods, a layer of Cu particles uniformly covers carbon paper by utilizing a magnetron sputtering means, Ni and Co particles are deposited on a Cu surface layer by utilizing an electrochemical deposition method, and a ternary alloy electrocatalyst is prepared; and the efficient increase of the chemical reaction rate is promoted by cooperating with the catalytic action of multi-metal multi-active sites. According to the invention, harmless treatment of NOx gas and synthesis of ammonia and nitric acid are realized, extremely high ammonia yield and selectivity are achieved, and high-valued resource utilization of pollutants containing nitrogen and the like is facilitated.

The invention discloses a kind of Acinetobacter maize ACZLY512 and its application, and relates to the technical field of agricultural microorganisms; the invention discloses a kind of Acinetobacter maize ACZLY512, which was preserved in China Microorganism Culture Collection on May 06, 2021 General Microbiology Center of the Management Committee, the preservation number is CGMCC No.22268; the Acinetobacter maize ACZLY512 disclosed by the present invention can significantly improve the nitrogenase activity and nitrogen fixation efficiency of nitrogen-fixing bacteria under aerobic or micro-aerobic conditions. It provides key supporting materials for enhancing the environmental adaptability of nitrogen-fixing microorganisms and improving nitrogen-fixing efficiency, and has an important inspiration for the future excavation and utilization of nitrogen-fixing bacteria in this field.

The invention discloses the preparation of a cobalt oxyhydroxide nanosheet-supported cobalt MOF composite catalyst and the application of nitrogen fixation to ammonia, and belongs to the technical fields of nanomaterials and electrochemical catalysis. The main steps are to use cobalt chloride as raw material to prepare black brown cobalt oxyhydroxide powder, and then use cobalt oxyhydroxide, cobalt nitrate and trimesic acid as raw materials to prepare cobalt oxyhydroxide nanosheets loaded with Co 3 (BTC) 2 Nanocrystalline Co 3 (BTC) 2 @CoOOH composites. The cost of raw materials used in the preparation of the catalytic material is low, the preparation process is simple, the reaction energy consumption is low, and it has industrial application prospects. The Co 3 (BTC) 2 The @CoOOH composite material is used for high-efficiency electrochemical nitrogen fixation reaction, and has good electrochemical catalytic performance and stability.

The invention relates to a specific promoter in root tissue of leguminous plants and its application. The promoter is particularly suitable for driving the specific expression of the exogenous gene in the root tissue of the legume plant, while the expression level in other tissues of the legume plant is extremely low or not expressed. The expression specificity and specificity of the promoter are very high. The present invention also provides constructs, expression cassettes, vectors and the like having the promoter. The transgenic legume plant containing the promoter of the present invention can specifically improve the agronomic traits of the legume plant, effectively avoid the impact of foreign gene introduction on other tissues of the legume plant, and can also specifically express the foreign gene in the root tissue, Thereby obtaining various related products.

The invention belongs to the technical field of plasma generating equipment, in particular to an air plasma nitrogen oxide synthesis device, comprising a high-voltage end fixed block and a grounded end fixed block, and the high-voltage end fixed block and the grounded end fixed block are respectively fixed with high-voltage electrodes and the grounding electrode, the side of the high-voltage end fixing block and the grounding end fixing block that are close to each other is fixedly installed with the same dielectric tube, and the ends of the high-voltage electrode and the grounding electrode that are close to each other extend into the medium tube, and the medium tube The fixed sleeve is provided with a heat dissipation mechanism and a cooling container, a displacement control platform is fixedly installed at the bottom of the ground electrode, and a high voltage power supply is connected to one side of the fixed block at the high voltage end. The invention uses an intersecting air flow field to promote the air to pass through the plasma region and participate in the plasma process to improve the generation efficiency of nitrogen oxides; it can be combined with an external cooling method to effectively reduce the high temperature in the plasma region and further improve the generation of nitrogen oxides efficiency.

The invention relates to a specific promoter in a leguminous plant root system tissue and application thereof. The promoter is particularly suitable for driving the specific expression of exogenous genes in the leguminous plant root tissue, and the expression level of the exogenous genes in other tissues of leguminous plants is extremely low or the exogenous genes are not expressed in the other tissues of the leguminous plants. The expression specificity and specificity of the promoter are very high. The invention further provides constructions, expression kits, carriers and the like with the promoter. For transgenic leguminous plants containing the promoter disclosed by the invention, agronomic traits of the leguminous plants can be specifically improved, so that the influence on the other tissues of the leguminous plants caused by the introduction of the exogenous genes is effectively avoided, the exogenous genes can be specifically expressed in the root tissue, and thus, various related products are obtained.

The invention belongs to the field of photocatalytic nitrogen fixation synthesis ammonia, and particularly relates to a construction method and application of a Pickering microbubble system for photocatalytic nitrogen fixation ammonia synthesis. The Pickering microbubble system disclosed by the invention is an N2-in-water microbubble system formed by taking an amphiphilic Pickering solid photocatalyst as an emulsifier and spontaneously assembling the amphiphilic Pickering solid photocatalyst on a gas-water interface. When the system is used for photocatalytic nitrogen fixation ammonia synthesis reaction, the nitrogen fixation efficiency can be improved by 5-40% in comparison with that of the traditional gas-liquid two-phase reaction, and the selectivity of ammonia can be improved by 10-50%.

The invention discloses a flat steel ingot for a corrosion-resistant mold and a process method thereof, belonging to the technical field of metallurgical production process. To provide a flat steel ingot for corrosion-resistant molds with high strength, corrosion resistance, wear resistance, creep resistance, and fatigue resistance, and the application also provides a process for producing the flat steel ingot for corrosion-resistant molds . The flat steel ingot is a metallurgical die-cast steel ingot containing the following components in parts by weight: C 0.25-0.65%, Si 0.4-0.8%, Mn 0.4-0.9%, Cr 12-18%, Ni 0.05-0.3% , Mo 0.1-0.3%, N 0.06-0.30%, Al≤0.02%, P≤0.020%, S≤0.005%, and the rest are Fe and unavoidable impurity elements. The process method includes several steps of smelting molten steel with a nitrogen content less than or equal to 45ppm, refining nitrogen-rich and manganese-rich molten steel, and die casting, wherein the refining of nitrogen-rich and manganese-rich molten steel includes large slag in a refining furnace Quantitative refining and secondary refining in vacuum refining furnace.

The invention discloses application of nKCBP protein in regulation and control of nitrogen fixation capacity of leguminous plants. The amino acid sequence of the nKCBP protein is as shown in SEQ ID No. 2. The inventor of the invention uses a CRISPR / Cas9 technology to edit the nKCBP gene at a fixed point and knock out the medicago truncatula nKCBP gene, so that the plant height and the nitrogen fixation capability are reduced. The invention is very important for researching the efficient nitrogen fixation mechanism of leguminous plants, and provides a new thought for improving the biological nitrogen fixation efficiency. The invention has important application value for plant breeding.

An Azospirillum brasilense DraT-engineering strain containing multi-copied nifA gene is disclosed, with preserving number of CGMCC No.0876. This bacterial manure containing engineering strain of Azospirillum brasilense DraT- with multi-copied nifA is also disclosed. The cultured engineering strain as bacterial manure is applied for field plant of corn and wheat to decrease use amount of urea, increase yield and save manure, promote plant growth by IAA.

The invention provides artificial tandem polypeptide ADPs for improving the nitrogen fixation efficiency of rhizobium, an expression vector and construction and application of recombinant rhizobium, and relates to the technical field of root nodule nitrogen fixation. A plurality of NCR polypeptides with similar isoelectric points are connected in series; the method comprises the following steps: constructing an artificial tandem polypeptide ADPs, inserting a coding gene of the artificial tandem polypeptide ADPs into pHC60 to form an expression vector, and then transforming Mesorhizobium japonicum MAFF303099 to obtain recombinant rhizobium, so that the artificial tandem polypeptide ADPs are expressed in the rhizobium. After the recombinant rhizobium is inoculated to lotus corniculatus, the thallus-like form can be obviously changed, and the nitrogenase activity can be improved.