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A kind of maize endophytic Acinetobacter aczly512 and its application

A technology of corn and bacilli, applied in the field of agricultural microorganisms

Active Publication Date: 2022-02-01
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the research on the synergistic effect of non-nitrogen-fixing bacteria on nitrogen-fixing bacteria in this field is still almost blank.

Method used

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  • A kind of maize endophytic Acinetobacter aczly512 and its application
  • A kind of maize endophytic Acinetobacter aczly512 and its application
  • A kind of maize endophytic Acinetobacter aczly512 and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation, screening and identification of embodiment 1 bacterial strain ACZLY512

[0033] 1.1 Isolation and screening of strain ACZLY512

[0034] (1) Strain ACZLY512 was derived from the sap of the stem conduction tissue of maize variety Yedan 13. Select the central position of the third stem above the base of the corn, and cut off the corn stem with pruning shears. Due to the root pressure of the corn, the juice of the corn stem conduction tissue will flow out from the cross-section, and use 0.5g sterile absorbent cotton balls to absorb the corn stem juice. For juice, put absorbent cotton balls into 50ml sterile centrifuge tubes, put them on ice and bring them back to the laboratory.

[0035] (2) Add 2 mL of 0.9% sterile normal saline to a centrifuge tube filled with absorbent cotton balls in a clean bench, incubate on a shaker at 28° C. and 160 rpm for 1 hour, and mix thoroughly. The cultured liquid was diluted 10 times, and 100 μL of the diluted solution was appli...

Embodiment 2

[0052] Example 2 Compared with other non-nitrogen-fixing bacteria, the promotion effect of bacterial strain ACZLY512 on the nitrogen-fixing function of endophytic nitrogen-fixing bacteria

[0053] 2.1 Nitrogen-fixing complex bacterial strain F and other 4 non-nitrogen-fixing comparison strains used for nitrogen-fixing effect verification test

[0054] In order to compare the effect of strain ACZLY512 and other non-nitrogen-fixing bacteria on endophytic nitrogen-fixing bacteria, three strains of nitrogen-fixing bacteria were selected and mixed in equal proportions to form a nitrogen-fixing compound strain F. In addition, in addition to the strain ACZLY512, four other non-nitrogen-fixing bacteria were selected as the comparison strains of the strain ACZLY512 to study the effect of adding non-nitrogen-fixing bacteria on the nitrogen fixation of nitrogen-fixing compound strain F. Except that the amount of oxygen added is different, the verification method of nitrogenase activity i...

Embodiment 3

[0077] Example 3 Promoting effect of bacterial strain ACZLY512 on the nitrogen fixation function of nitrogen-fixing bacteria

[0078] In order to verify the effect of strain ACZLY512 on the nitrogen-fixing function of nitrogen-fixing bacteria, three strains of nitrogen-fixing bacteria were selected, namely Klebsiella sp. MNAZ1050 (indicated by A in Figure 8-13), Citrobacter sp. .) MNAZ1397 (indicated by B in Figure 8-13) and Pseudomonas (Pseudomonas sp.) MNAZ228 ( Figure 8-13 Indicated by C).

[0079] 3.1 Determination of Nitrogenase Activity under 1% Microaerobic Condition

[0080] 3.1.1 Add the strain ACZLY512 at the ratio of nitrogen-fixing bacteria: non-nitrogen-fixing bacteria = 1:1

[0081] The nitrogenase activity of Klebsiella MNAZ1050(A) is 1261.6nmol C 2 h 4 / mg pro hr, when Klebsiella MNAZ1050 was added with strain ACZLY512 at a ratio of 1:1 (A+b), its nitrogenase activity was as high as 1612.0nmol C 2 h 4 / mg pro hr, compared with Klebsiella MNAZ1050(A), th...

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Abstract

The invention discloses a kind of Acinetobacter maize ACZLY512 and its application, and relates to the technical field of agricultural microorganisms; the invention discloses a kind of Acinetobacter maize ACZLY512, which was preserved in China Microorganism Culture Collection on May 06, 2021 General Microbiology Center of the Management Committee, the preservation number is CGMCC No.22268; the Acinetobacter maize ACZLY512 disclosed by the present invention can significantly improve the nitrogenase activity and nitrogen fixation efficiency of nitrogen-fixing bacteria under aerobic or micro-aerobic conditions. It provides key supporting materials for enhancing the environmental adaptability of nitrogen-fixing microorganisms and improving nitrogen-fixing efficiency, and has an important inspiration for the future excavation and utilization of nitrogen-fixing bacteria in this field.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a kind of Acinetobacter maize endogenous ACZLY512 and its application. Background technique [0002] Nitrogen-fixing microorganisms are microorganisms that can reduce molecular nitrogen to ammoniacal nitrogen. The total amount of nitrogen-fixing microorganisms in the world exceeds 50 million tons per year. Combined nitrogen fixation refers to the colonization of nitrogen-fixing bacteria on the surface or inside of plants, using carbohydrates produced by host plants as energy substances, and performing biological nitrogen fixation when conditions are suitable, providing nitrogen sources for host plants, so that the two form a good mutualistic symbiotic relationship . The use of combined nitrogen-fixing bacteria can realize the autonomous biological nitrogen fixation of non-legume crops, and meet the nitrogen nutrition requirements for the healthy growth of cro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01C12R1/065
CPCC12N1/20
Inventor 艾超张俪予周卫
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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