Human protein phosphokinase 2A point mutanted lung cancer related antigen gene

A technology of phosphatase and human protein, applied in recombinant DNA technology, DNA/RNA fragments, instruments, etc.

Inactive Publication Date: 2004-12-22
昆明医学院第一附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is no report on the discovery and cloning of the gene encoding the mutant v

Method used

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  • Human protein phosphokinase 2A point mutanted lung cancer related antigen gene
  • Human protein phosphokinase 2A point mutanted lung cancer related antigen gene
  • Human protein phosphokinase 2A point mutanted lung cancer related antigen gene

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0020] Obtaining the sequence described in the present invention comprises the following steps:

[0021] 1. Materials

[0022] 1.1 Cell lines: Yunnan Gejiu lung adenocarcinoma GLC-82 cells and hybridoma P35 cells, (the establishment and characteristics analysis of Yunnan Gejiu lung adenocarcinoma cell line GLC-82. Chinese Journal of Tumors, 1985, 7(2): 81 -83)

[0023] 1.2 Anti-human lung cancer monoclonal antibody N35: self-made by our laboratory (543 cases of anti-human lung cancer monoclonal antibody development and immunohistochemical analysis were recorded. Chinese Journal of Oncology, 1992, 14(6): 473)

[0024] 1.3 Human small cell lung cancer cell H128 cDNA library constructed in λgt11 vector and its host bacteria (Escherichia coli Y1090). (Provided by Shanghai Cancer Institute)

[0025] 2 steps

[0026] 2.1 Immune reaction between N-35 antibody and host bacteria Y1090 bacterial protein, to detect whether anti-E. coli antibody exists in N-35.

[0027] (1) Prepare E...

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Abstract

A human protein phosphatase site-2A alpha mutant gene sequence associated with lung cancer, the change from arginine to tryptophane in the polypeptide chain whose amino acid sequence is shown by SEQ ID No.2, and the process for preparing said DNA sequence are disclosed, which can be used for diagnosing and treating lung cancer.

Description

(technical field) [0001] The present invention relates to lung cancer-associated antigen mutant gene sequence, in particular to the coding gene of mutant human protein phosphatase 2A (protein phosphatase 2A, PP2A) α catalytic subunit. (Background technique) [0002] Lung cancer is one of the most common malignant tumors in human beings. In recent years, the morbidity and mortality have shown an obvious upward trend both at home and abroad, but there is still a lack of effective treatment methods. It is now believed that tumor is a malignant disease with multiple gene changes. Its occurrence and development are closely related to a series of tumor-related gene structure, function changes, and abnormal regulatory relationships. It is a multi-stage, multi-step, and multi-gene process. Protein phosphatase refers to a class of enzyme molecules that catalyze the dephosphorylation reaction of phosphorylated protein molecules. They exist corresponding to protein kinases and together...

Claims

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Application Information

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IPC IPC(8): C12N15/52C12Q1/68G01N33/577
Inventor 王秦秦马丽菊李继梅唐睿珠李海红
Owner 昆明医学院第一附属医院
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