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Application of agglutinin II protein of rhizome of manyflower solmonaeal in pharmacy

A technology of lectin and Polygonatum, which is applied in the field of Rhizoma Polygonatum Lectin II protein, can solve problems such as difficult to design drugs, achieve good medicinal prospects, wide sources, and reduce production costs

Inactive Publication Date: 2005-01-12
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment method has also changed from the traditional single drug therapy to a combination of multiple drugs, but it is still difficult to design a long-term drug against this retrovirus

Method used

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  • Application of agglutinin II protein of rhizome of manyflower solmonaeal in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Preparation of Polygonatum Lectin II Protein

[0017] In this embodiment, the preparation method of the luteinum lectin II protein is as follows:

[0018] 1. Crude product preparation

[0019] Extract sliced ​​Polygonatum tuber with normal saline solution (G / V=1:5) overnight, mash it into a paste with a high-speed tissue grinder, soak it with normal saline solution overnight, filter with gauze, and centrifuge (4°C) , 4500r / min, 30min), add solid ammonium sulfate to the supernatant to reach 30% saturation, place at 4°C overnight, centrifuge (4°C, 4500r / min, 30min) to remove precipitation, continue to add ammonium sulfate to the supernatant to 90%, The precipitate was collected by centrifugation, dissolved in distilled water, dialyzed to remove salt, and freeze-dried to obtain a crude sealogen lectin.

[0020] 2. Affinity column separation and purification

[0021] Take 200 mg of the crude product and dissolve it in 20 ml of normal saline solution, dialyz...

Embodiment 2

[0024] Example 2: Preparation of Polygonatum Lectin II Protein

[0025] In this embodiment, the preparation method of the luteinum lectin II protein is as follows:

[0026] 1. Crude product preparation

[0027] Extract sliced ​​and dried Rhizoma Polygonatum tubers with normal saline solution (G / V=1:5) overnight, mash it into a paste with a high-speed tissue grinder, soak with normal saline solution overnight, filter with gauze, and centrifuge (4°C, 4500r / min, 30min), add solid ammonium sulfate to the supernatant to reach 30% saturation, place at 4°C overnight, centrifuge to remove the precipitate as in the previous method, continue to add ammonium sulfate to the supernatant to 90%, and collect the precipitate by centrifugation. The precipitate was dissolved in distilled water, dialyzed to remove salt, and freeze-dried to obtain the crude sealogen lectin.

[0028] 2. Affinity column separation and purification

[0029] The crude product (200 mg) was dissolved in 20 ml of nor...

Embodiment 3

[0034] Example 3: Inhibitory effect of PCL II protein (PCL II) on hepatitis B (HBV) virus

[0035] 1. Materials and methods

[0036] 1.1 Materials

[0037] Polygonatum lectin II protein (PCL II) was prepared by the method described in Example 1 or Example 2, and was used for the determination of cytotoxicity and anti-hepatitis B activity after serial dilution.

[0038] Freeze-dried HBsAg diagnostic blood cells: Shanghai Industrial Kehua Bioengineering Co., Ltd.

[0039] Recombinant yeast hepatitis B virus vaccine (HBsAg): product of Beijing Institute of Biological Products, Ministry of Health.

[0040] HepG2.2.1.5 cells: cell lines provided by Hepatitis Research Institute of Chongqing Medical University. HBsAg, HBeAg detection kits: products of Beijing Sihuan Biological Engineering Products Factory.

[0041] Culture medium: RPMI-1640 (GIBCO product) was dissolved according to the instructions, adjusted to pH 7.2, filtered and sterilized, and fetal bovine serum (GIBCO produ...

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Abstract

An application of the polygonalectin II protein is preparing the medicine for preventing and reating hepatitis B and AIDS is disclosed as it can effectively suppress the HepG2.2.1.5 cell to secret antigens HBeAg and HBsAg, and the activity of HIV.

Description

technical field [0001] The present invention relates to the application of Polygonatum cyrtonema Hua., a Chinese herbal medicine, which is a liliaceous plant, and particularly relates to the application of Polygonatum cyrtonema Hua. in pharmacy. Background technique [0002] Lectin is a kind of non-enzyme, non-immune-derived protein or glycoprotein that has sugar-binding specificity and can precipitate sugar complexes widely in nature. Since the first plant lectin was found in castor bean, hundreds of lectins have been discovered and isolated. [0003] Polygonatum cyrtonema Hua., a plant of the Liliaceae family, is a traditional Chinese herbal medicine in my country. Bao Jinku et al. published a paper on "Journal of Biochemistry" (Volume 12, No. 6, December 1996) "The stability of Polygonatum cyrtonema lectin II molecule "Research on Sexuality and Biological Activity" discloses the mitogenic activity of Polygonatum agglutinin II prepared f...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P1/16A61P31/12A61P31/18
Inventor 鲍锦库吴传芳安洁邓洁何晓佳洪志霞吕鸿周刘超陈放
Owner SICHUAN UNIV
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