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Hybrid method of Afria crucia carp and mandarin fish

A hybridization method, tilapia technology, applied in animal husbandry and other fields, can solve problems such as difficulty in obtaining mature eggs and uncontrollable spawning time, and achieve the effect of short body size, high survival rate and suitable for processing

Inactive Publication Date: 2005-02-23
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Since tilapia reproduction is natural spawning, it is impossible to control the spawning time, and it is difficult to obtain mature eggs for experiments such as hybridization, which can only be used for general production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The present invention moves annual tilapia from the overwintering room into the field pond, and strengthens feeding for about 1 month; meanwhile, it also strengthens the cultivation of mandarin fish (male) parent fish to ensure that there are sufficient and palatable forage fish in the pond. From the end of May, the broodstock of tilapia are basically mature and can be used for hybridization of oria tilapia (female) and mandarin fish (male). When the water temperature reaches 26°C or higher, pull the net to check the gonad development of Oria tilapia. Select a purebred Olia tilapia with a full abdomen, protruding genital openings that are red or reddish, strong and in good shape as the female parent of the hybrid, and the male parent of the hybrid is selected to have milky white semen flowing out when the abdomen is lightly pressed. For mandarin fish, a sufficient number of male Olia tilapia is selected according to the male-to-female ratio of 3:1. These broodstock wer...

Embodiment 2

[0031] When the water temperature is above 26°C, select the purebred Olia tilapia as the female parent of the hybrid, and select the male mandarin fish with milky white semen as the hybrid male parent, and select a sufficient number of male Olia at the same time according to the ratio of male to female at 3:1. Asian tilapia. These broodstock were placed in indoor aquariums, aerated to ensure dissolved oxygen. Using artificial injection of oxytocic agent, oxytocic agent is: human chorionic gonadotropin (HCG) dose is 350 units / kg fish body weight, luteinizing releasing hormone analogue (LRH-A 2 ) dose is 3.5 micrograms / kg fish body weight, dioconone (DOM) dose is: 2.5 mg / kg fish body weight, fish pituitary gland (PG) dose is: 5 mg / kg fish body weight. Using the hybridization method in Example 1, hybrid fish can also be successfully cultivated.

Embodiment 3

[0033]When the water temperature is above 26°C, sexually mature tilapia is selected as the female parent of the hybrid, and the male parent of the hybrid is the male mandarin fish with milky white semen, and the mature eggs are obtained by artificial injection of oxytocic agent, and the hybrid is carried out with the mandarin fish . Artificial injection of oxytocic agents were: human chorionic gonadotropin (HCG) at a dose of 400 units / kg fish body weight, luteinizing releasing hormone analog (LRH-A 2 ) dose is 5 micrograms / kg fish body weight, dioconone (DOM) dose is: 3 mg / kg fish body weight, fish pituitary gland (PG) dose is: 6 mg / kg fish body weight. And adopt the hybridization method among the embodiment 1,2, can successfully breed and obtain hybrid fish equally.

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PUM

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Abstract

A hybridization method between blue tilapia and mandarinfish includes such steps as choosing the blue tilapia as female parent and the mandarinfish as male parent, injecting echolica, squeezing the sperm of male parent to the ova of female one, incubating, raising in aqueium, and raising in pond.

Description

Technical field: [0001] The invention relates to a hybridization method of Oria tilapia and mandarin fish, which belongs to the necessities of human life [0002] technology field. Background technique: [0003] Before the present invention was made, in the prior art, Oria tilapia and mandarin fish usually adopt the following methods to reproduce: [0004] 1. Breeding of Oria tilapia: [0005] The reproduction of Olia tilapia is relatively complicated, and natural spawning is still used at present. In terms of production, the Olia tilapia used for breeding is placed in the spawning pond according to the ratio of male to female at 3:1, and the stocking density is generally about 600 broodstock of 250-300 g / tail per 1 / 15 hectare. Carnivorous fish shall not be mixed in the pond. In southern Jiangsu, from the end of May every year, tilapia eggs reach maturity, and they can reproduce on their own when the pond water temperature reaches 20°C or higher. After the female fish o...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 夏德全吴婷婷杨弘
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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