Hybrid method of Afria crucia carp and mandarin fish
A hybridization method, tilapia technology, applied in animal husbandry and other fields, can solve problems such as difficulty in obtaining mature eggs and uncontrollable spawning time, and achieve the effect of short body size, high survival rate and suitable for processing
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Embodiment 1
[0027] The present invention moves annual tilapia from the overwintering room into the field pond, and strengthens feeding for about 1 month; meanwhile, it also strengthens the cultivation of mandarin fish (male) parent fish to ensure that there are sufficient and palatable forage fish in the pond. From the end of May, the broodstock of tilapia are basically mature and can be used for hybridization of oria tilapia (female) and mandarin fish (male). When the water temperature reaches 26°C or higher, pull the net to check the gonad development of Oria tilapia. Select a purebred Olia tilapia with a full abdomen, protruding genital openings that are red or reddish, strong and in good shape as the female parent of the hybrid, and the male parent of the hybrid is selected to have milky white semen flowing out when the abdomen is lightly pressed. For mandarin fish, a sufficient number of male Olia tilapia is selected according to the male-to-female ratio of 3:1. These broodstock wer...
Embodiment 2
[0031] When the water temperature is above 26°C, select the purebred Olia tilapia as the female parent of the hybrid, and select the male mandarin fish with milky white semen as the hybrid male parent, and select a sufficient number of male Olia at the same time according to the ratio of male to female at 3:1. Asian tilapia. These broodstock were placed in indoor aquariums, aerated to ensure dissolved oxygen. Using artificial injection of oxytocic agent, oxytocic agent is: human chorionic gonadotropin (HCG) dose is 350 units / kg fish body weight, luteinizing releasing hormone analogue (LRH-A 2 ) dose is 3.5 micrograms / kg fish body weight, dioconone (DOM) dose is: 2.5 mg / kg fish body weight, fish pituitary gland (PG) dose is: 5 mg / kg fish body weight. Using the hybridization method in Example 1, hybrid fish can also be successfully cultivated.
Embodiment 3
[0033]When the water temperature is above 26°C, sexually mature tilapia is selected as the female parent of the hybrid, and the male parent of the hybrid is the male mandarin fish with milky white semen, and the mature eggs are obtained by artificial injection of oxytocic agent, and the hybrid is carried out with the mandarin fish . Artificial injection of oxytocic agents were: human chorionic gonadotropin (HCG) at a dose of 400 units / kg fish body weight, luteinizing releasing hormone analog (LRH-A 2 ) dose is 5 micrograms / kg fish body weight, dioconone (DOM) dose is: 3 mg / kg fish body weight, fish pituitary gland (PG) dose is: 6 mg / kg fish body weight. And adopt the hybridization method among the embodiment 1,2, can successfully breed and obtain hybrid fish equally.
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