Process for preparing bio- red selenium

A preparation process and biological technology, applied in the field of biological medicine, to achieve the effect of eliminating depression, enhancing physical strength, and accelerating apoptosis

Inactive Publication Date: 2005-04-20
张敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the utilization of selenium is greatly limited, and it is a subject in research to strive to develop high-efficiency and low-toxicity selenium sources

Method used

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  • Process for preparing bio- red selenium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of preparation technology of biological red selenium can be carried out successively in the following order:

[0025] (A) Bacterial solution preparation;

[0026] Selenophilic ZM bacteria were inoculated in 1.5% peptone liquid medium, cultured at 37° C. for 24 hours, and set aside.

[0027] (B) preparation of selenium;

[0028] Proceed in the following order:

[0029] 1) In 50ml of 1% peptone liquid culture medium, add the above-mentioned bacterial solution and 1ml of 4% sodium selenite solution respectively, cultivate at 24°C, the fermentation broth turns from light yellow to red, and red ZM bacteria can be seen under the microscope slide;

[0030] 2) 20 mg / ml (as Na 2 SeO 3 5H 2 O meter) red bacteria selenium.

[0031] The peptone liquid culture medium in the preparation of the bacteria liquid in item A contains: 0.1% dipotassium hydrogen phosphate K 2 HPO 4 3H 2 O.

Embodiment 2

[0033] The preparation process of the present invention is characterized in that it is carried out in the following order:

[0034] (A) Bacterial solution preparation;

[0035] Selenophilic ZM bacteria were inoculated in 1.5% peptone liquid medium, cultured at 37° C. for 24 hours, and set aside.

[0036] (B) preparation of biological red selenium;

[0037] Proceed in the following order:

[0038] 1) In 1000ml of 1-2% peptone liquid culture medium, add the above-mentioned bacterial solution and 10ml of 5% sodium selenite solution respectively, cultivate at 40°C, the fermentation broth changes from light yellow to red, and red can be seen under the microscope ZM bacteria sliding;

[0039] 2) After concentrated treatment, 50mg / ml (as Na 2 SeO 3 5H 2 O meter) red bacteria selenium.

[0040] The peptone liquid culture medium in the preparation of the bacteria liquid in item A contains: 0.1% dipotassium hydrogen phosphate K 2 HPO 4 3H 2 O.

[0041] Curative effect of th...

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Abstract

Said invention relates to process for preparing biological red selenium belonging to biomedicine field. The process is that, (A), bacterial liquid preparation, seleniumphilous ZM bacterial is inoculated in 1.5 % of peptone liquid medium at 37 degree centigrade for 24 hr, (B), biological red selenium preparation, 1,adding above mentioned bacterial liquid and 4-5 % of sodium selenium into 1-5 % of peptone liquid medium 50-1000 ml at 24-40 degree centigrade to culture, the fermentation liquid color changes from buff into red, the ZM bacterial can be seen to move by microscope, 2, through concentration to obtain 20-50 mg red bacterial selenium.

Description

Technical field [0001] The invention belongs to the field of biological medicine, in particular to a preparation process of biological red selenium. Background technique [0002] Selenium was discovered and named by the Swedish chemist Bezeliu in 1817. In 1957, Schwarz and Foltz first proved that selenium is an essential trace element for animals and humans. In the 1980s, Chinese medical scientists first applied selenium supplementation to prevent and treat Keshan disease, and Kashin-Beck disease achieved remarkable results. A lot of work has confirmed that selenium has a wide range of biological effects, and selenium deficiency can cause a series of diseases: cancer, AIDS, cardiovascular disease, liver and pancreas disease, hypertension, diabetes, anemia, Keshan disease, etc. [0003] A series of biochemical effects of selenium are closely related to its concentration. The anticancer effect of selenium requires a higher concentration, and the toxicity of selenium compound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P35/00C12N1/20C12P1/04
Inventor 张敏刘晓涛
Owner 张敏
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