Application of protein finger mark in blood by detecting myocardial infarction using protein finger mark

A fingerprinting and protein technology, applied in the field of protein fingerprinting and protein fingerprinting of biological sample analysis, can solve problems such as difficulty in distinguishing biomolecules, limited gel capacity and sensitivity, and inability to detect biomolecules.

Inactive Publication Date: 2006-02-22
BEIJING SELDI TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the method provides only two characteristics of biomolecules—mass and isoelectric point (pI)
Second, the resolution in each dimension is limited by the resolving power of the gel
For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI
Third, gels have limited capacity and sensitivity and may not be able to detect small amounts of expressed biomolecules
Fourth, small proteins or peptides with a molecular weight below about 10-20 kDa cannot be observed
Since human cell proteins are much more complex than potatoes, electrophoresis alone to identify protein fingerprints of diseases is far from meeting clinical needs

Method used

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  • Application of protein finger mark in blood by detecting myocardial infarction using protein finger mark
  • Application of protein finger mark in blood by detecting myocardial infarction using protein finger mark

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Example 1 Distinguishing Protein Fingerprints of Normal Human Cells, Unstable Angina Pectoris Cells and Acute Myocardial Infarction Cells in Blood

[0064] (1) Experimental method

[0065] 1. Materials

[0066] 1. Source of specimens: 99 patients in the acute myocardial infarction group were all diagnosed as acute myocardial infarction by electrocardiogram and myocardial enzyme spectrum. Among them, blood was collected immediately in 45 cases upon admission, in 6 cases after 3 hours, and in 11 cases after 6 hours. Blood samples were taken from 12 cases after 9 hours, 12 cases after 12 hours, 8 cases after 24 hours, and 5 cases after 48 hours; 45 cases of normal people in the normal group; 21 cases in the unstable angina pectoris group. All three groups were between 40 and 60 years old and had similar exposure histories. Ratio of male to female: In the acute myocardial infarction group, there were 56 males and 43 females; in the unstable angina group, there were 12 mal...

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Abstract

This invention also relates to protein dactylography method for analyzing for the protein group got by retention chromatography. This method adopts mass spectroscopy to distinguish and detect protein group. The analysis data takes the form of bar code (protein dactylography) read by computer. This method can be used in the test of normal cells and unstable angina cells and emergent myocardial infarction cells.

Description

technical field [0001] The invention relates to a novel method for analyzing proteins in biological samples, in particular to a protein fingerprint method for analyzing biological samples. The present invention also relates to protein fingerprinting in which the proteome is captured by retention chromatography and analyzed using a computer readable barcode format (protein fingerprint). Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed within cells can be used in the diagnosis of disease and ultimately in drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations ...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/95G01N30/92G06F19/00
Inventor许洋高春芳
OwnerBEIJING SELDI TECH