Breeding method for cross breeding glutinous rice
A technology of hybrid rice and glutinous rice, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of glutinous rice that cannot meet the requirements of high yield, wide adaptability and quality for multiple purposes, low yield, resistance, etc. Problems such as poor traits, to achieve the effect of saving manpower, long breeding cycle, and shortening the breeding cycle
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Embodiment 1
[0022] Example 1 The cultivation example of the hybrid glutinous rice Teyou 63 waxy is not detailed enough, the same below
[0023] The parents of Teyou 63 hybrid rice were used as materials, in which the restorer line was Minghui 63, the maintainer line was Longtepu B, and the sterile line was Longtepu A. With a dose rate of 1Gy / min, a dose of 350Gy 60 Co gamma irradiated Minghui 63 dry seeds 500g, M 1 Densely planted clusters to ensure that M 1 10,000 seedlings and 30,000 spikes or more. Mixed M 2 Seeds are removed from the hulls, and the glutinous mutant rice grains in the endosperm are screened from the brown rice population. Seedlings are grown on MS medium, and then transplanted in the field for planting identification. Select excellent lines with various traits similar to Minghui 63, and harvest m 3 The generation seeds are the waxy restorer line Minghui 63wxR.
[0024] The same dose rate of 1Gy / min, 450Gy dose 60 Dry seeds of Coγ-ray irradiated maintainer line L...
Embodiment 2
[0027] Example 2 Cultivation of hybrid glutinous rice Liangyou 2186 waxy
[0028] The parents of Liangyou 86 hybrid rice were used as materials, in which the restorer line was Minghui 86, and SE21S was a photothermosensitive genic male sterile line. With a dose rate of 5Gy / min, a dose of 600Gy 60 Co gamma ray irradiated Minghui 86 dry seeds 500g, M 1 Densely planted clusters to ensure that M 1 10,000 seedlings and 30,000 spikes or more. Mixed M 2 Seeds are removed from the hulls, and the glutinous mutant rice grains in the endosperm are screened from the brown rice population. Seedlings are grown on MS medium, and then transplanted to the field for planting identification. Select lines with excellent traits and harvest M 3 The generation seeds are the waxy restorer line Minghui 86wxR.
[0029] Select 50 kg of SE21S naturally propagated seeds, remove the hulls and remove the roughness, and screen out 3 grains of naturally mutated waxy brown rice, grow seedlings according t...
Embodiment 3
[0031] Example 3 Cultivation of the hybrid glutinous rice Dyou 527 waxy
[0032] The parents of Dyou 527 hybrid rice were used as materials, in which the restorer line was Shuhui 527, the maintainer line was D62B, and the sterile line was D62A. Use returnable satellite to carry 100 grams of dry seeds of Shuhui 527, sow S in the field 1 , mix S 2 Seeds are removed from the hulls, and the glutinous mutant rice grains in the endosperm are screened from the brown rice population. Seedlings are grown on MS medium, and then transplanted to the field for planting identification. Select lines with excellent traits and harvest F 3 The generation seeds are the waxy restorer line Shuhui 527wxR.
[0033] With a dose rate of 0.5Gy / min, a dose of 300Gy 60 Coγ-ray irradiation maintainer D62B dry seeds (M 1 )500g, M 1 Densely planted clusters to ensure that M 1 10,000 seedlings and 30,000 spikes or more. Mixed F 2Seeds are removed from the husks, and the glutinous mutant rice grains i...
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