Agricultural waste fermenting bacterial prepn and its prepn process

A bacterial strain, the technology of Streptomyces luteus, is applied in the field of bacterial preparation and the preparation of the bacterial preparation, which can solve the problems of insufficient fermentation, incomplete killing of harmful organisms, and low fermentation pile temperature, etc.

Inactive Publication Date: 2006-05-17
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

Because the temperature of the fermentation heap is not high, the fermentat

Method used

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  • Agricultural waste fermenting bacterial prepn and its prepn process
  • Agricultural waste fermenting bacterial prepn and its prepn process
  • Agricultural waste fermenting bacterial prepn and its prepn process

Examples

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manufacture example

[0043] 1. Preparation of ZJY2 inocula:

[0044] ZJY2 Liquid Shake Flask Medium for Fructus Dimosus ZJY2: 1000ml wort (10°Bx), 18g glucose, natural pH. Add 20 grams of agar as the medium to prepare a solid mother seed slant for inoculation and culture. According to the aseptic method, take a loop of the slant mother seed and inoculate it in a yeast liquid shake flask (60ml medium / 250ml Erlenmeyer flask) medium. , 28℃, rotation speed 180rpm, culture for 18 hours, the number of bacteria reaches 0.5×10 8 More than cfu / ml is fine. The solid fermentation medium of Fructus saccharomyces cerevisiae: with 99% bran and a small amount of inorganic salt MgSO 4 , NaCl 0.05% each, add water according to the material to water ratio 1:1 (w / w) to make the material water content about 60%. Take the liquid bacteria cultured in the shake flask as the strain, and inoculate it evenly in the solid fermentation koji (the koji is wooden, the length, width and height are 60cm, 45cm, and 6cm respectively, a...

experiment example

[0063] deal with

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Abstract

The present invention discloses fermented bacteria preparation for agricultural waste treatment and its preparation process. The present invention separates and breeds three kinds of bacteria with fast growth and high heat resistance, including two strains of tectorial membrane yeast ZJY2 and Strreptomyces griseus W1 and one known strain Bacillus cereus ME1-2. These three kinds of spawn are cultured and fermentation produced separately and further produced into compound bacteria preparation in certain ratio or single bacteria preparation. When the bacteria preparation is used in treating agricultural waste, the waste is added with regulator to control the water content in 55-65 wt% and the bacteria preparation in 0.1-0.5 wt% is added through stirring, and the mixture is aerobic high temperature fermented for 20-30 days to reduce amount, reduce harm and become useful resource.

Description

[0001] This application is a divisional application of the original application date: March 18, 2004, application number: 200410039818.0, and the invention titled "Fermentation inoculum for agricultural waste treatment and its preparation method". Technical field [0002] The invention belongs to the field of fermentation inoculum for processing wastes from agriculture, animal husbandry and food processing and its manufacture, in particular to an inoculum capable of accelerating the fermentation of materials, and a preparation method and application of the inoculum. Background technique [0003] The large-scale livestock and poultry breeding industry in my country has developed rapidly in the past ten years. In particular, the pig industry with a high degree of intensive breeding has developed rapidly, but it has also caused serious environmental pollution while increasing the level of production. . In addition, a large amount of c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/465
Inventor 薛智勇王卫平朱凤香钱红吴传珍何福恒章锦秋
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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