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Pear virus hybridization detection kit and detection method thereof

A detection kit, detection method technology, applied in biological testing, biochemical equipment and methods, measuring devices, etc., to achieve the effects of easy operation, good stability and high sensitivity

Inactive Publication Date: 2006-10-04
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the maturity of molecular biology technology, many methods have been applied to the detection of fruit tree virus, but there is no research on the molecular detection kit of fruit tree virus.

Method used

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  • Pear virus hybridization detection kit and detection method thereof
  • Pear virus hybridization detection kit and detection method thereof
  • Pear virus hybridization detection kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The hybrid detection kit and detection method thereof of the pear ring mosaic virus on the pear tree specifically include as follows;

[0053] The pear ring mosaic virus probe sequence is shown in the sequence (1) in the sequence listing.

[0054] The amount of the above probe is; 0.005-0.03 mg / mL DNA.

[0055] 1# solution; pre-hybridization solution, containing: 50% deionized formamide, 5×Denhardt’s, 0.1% sodium dodecyl sulfate, 5×SSC, 20mmol / L sodium phosphate, 0.5mg / mL salmon sperm DNA, 20mL.

[0056] 2# solution; hybridization solution, containing, 50% deionized formamide, 5×Denhardt’s, 0.1% sodium dodecyl sulfate, 5×SSC, 20mmol / L sodium phosphate, 0.5mg / mL salmon sperm DNA, 20mL 20 mL of prehybridization solution containing 0.005-0.030 mg / mL DNA probe,

[0057] 3# solution; Cleaning solution I#, 2×SSC containing 0.1% sodium lauryl sulfate, 40mL,

[0058] 4# solution; cleaning solution II#, 0.1×SSC containing 0.1% sodium dodecyl sulfate, 40mL

[0059] 5# liquid; ...

Embodiment 2

[0084] The difference between this example and Example 1 is that the sequence of the pear and apple stem groove virus probe is shown in the sequence (2) in the sequence list.

[0085] The amount of the above probe is; 0.005-0.03 mg / mL DNA.

[0086] Result observation and recording:

[0087] The virus appears as a basket-purple spot at the spotting site.

Embodiment 3

[0089] The difference between this example and Example 1 is that the probe sequence used for piriweihuang virus is shown in the sequence (3) in the sequence list.

[0090] The amount of the above probe is; 0.005-0.03 mg / mL DNA.

[0091] Result observation and recording:

[0092] Viruses appear as bluish-purple spots at the spots.

[0093] sequence listing

[0094] Shihezi University

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Abstract

The related pear tree virus hybridization detection reagent box comprises the pre-hybridizing liquid, the hybridizing liquid, the cleaning solution I / II, the buffer liquid I / II / III, the antigen liquid, the primer, and probe. This invention has well reliability, high sensitivity, and strong specificaity, needs only low cost and simple operation, and has wide application.

Description

technical field [0001] The invention relates to a pear tree virus hybridization detection kit and a detection method thereof. Background technique [0002] The traditional methods for detection and identification of virus in fruit trees are indicator plant method and serological method. After decades of screening, a complete set of standard indicator plants has been confirmed internationally. Although this method is highly reliable for the identification of currently known fruit tree viruses, it takes a long time and is labor-intensive and land-consuming. Compared with the indicator plant method, the serological method has greatly improved the sensitivity, detection speed and specificity of fruit tree virus detection. It has become a routine method for fruit tree virus detection and identification. There are many reports on this aspect at home and abroad. However, this detection method has high detection cost due to the difficulty in preparing antiserum for fruit tree viru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/53G01N33/569G01N21/78C12Q1/00C07H21/00
Inventor 牛建新马兵钢
Owner SHIHEZI UNIVERSITY
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