Medium for culturing grown-up cockscombin sealed vessel, and culture method for induction blossom
A technology of airtight container and culture medium, which is applied in the field of flowering culture medium and induced flowering culture of cockscomb in airtight container, which can solve the problems of low flowering rate of foliage flowers
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[0020] The most suitable culture condition of Celosia chinensis in airtight container and its flowering induction culture process.
[0021] 1. Establish a sterile vaccine system:
[0022] The cockscomb seeds are used as explants, and after the surface is cleaned, they are rinsed under running water for 3-4 hours. Then put it on a clean workbench for disinfection and inoculation. The disinfection method is the conventional disinfection method, ① 75% alcohol for 2-3 seconds, ② 0.1% bromogeramine for 10-15 minutes, ③ 0.1% mercury liter for 2-5 minutes , ④ rinse with sterile water 4-5 times. Wipe the inoculation tool with alcohol cotton, and close to the alcohol lamp for barbecue disinfection, and inoculate the explant of Celosia celosia on the prepared differentiation medium for differentiation culture.
[0023] The medium used in this process is: MS+BA1-1.5mg / L+IBA0.5-1.0mg / L+30% sugar+7g / L agar, pH5.8. The culture conditions are as follows: temperature is about 20-24°C, ligh...
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