Method for determining dual-purpose line fertility by use of leaf marking trait in seedbed stage

A technology of dual-purpose lines and leaves, applied in the direction of botany equipment and methods, applications, and the use of vectors to introduce foreign genetic materials, etc., can solve the problems of single cytoplasmic sterile line restorer line, complicated and time-consuming seed production procedures, and yield advantages Inconspicuous and other problems, to achieve high purity, high commercial value, reduce manpower consumption and economic costs

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Problems solved by technology

The research and utilization of cotton heterosis is at the leading level in the world. In the existing hybrid cotton seed production methods, manual detasseling requires manual removal of pollen, which is a large workload and high cost of seed production; the restorer line of the cytoplasmic sterile line is single , the yield advantage is not obvious, and it has not been widely used in production; the fertility stability of the "secondary method" using nuclear male sterile lines needs to be further studied, and it is only used on a small scale; only using nuclear male sterile lines The hybrid cotton prepared by the "two-line method" of the line is widely used (recently approved varieties include Chuanza 12, Chuanza 13, Chuanzamian 14, Chuanzamian 15, etc.)
However, because the dual-purpose lines for seed production need to be identified for fertility when they bloom, and the fertile plants are removed after the identification of fertility in the field flowering stage, the seed production procedure is complicated, labor-intensive and time-consuming, and it is difficult to popularize. The uneven distribution in the field will affect the yield of seed production, and the fertile plants will be pulled out after flowering, which will waste a lot of water and fertilizer resources and make the cost of seed production high

Method used

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Effect test

Embodiment 1

[0016] A method for identifying the fertility of dual-purpose lines using leaf marker traits seedbed, the method is:

[0017] A. The GMS line was anti-A 3 MSC 1 and restorer line B with purple leaf marker traits 12 The purple leaves control the dominant gene B as a molecular marker.

[0018] B. On the basis of molecular markers, cloning of MSCs with dominant alleles of fertility control genes 1 and purple leaf control dominant gene B, construct the fertile dominant allele MSC of the fertility control gene 1 Linkage genome MSC linked with purple leaf control dominant gene B 1 b.

[0019] C. Linking genomic MSCs 1 B was bred into a high-yield and high-quality cotton GMMS line to cultivate a GMMS line purple A with purple leaf marker traits 1 (MSC 1 msc 1 Bb); Violet A 1 Among the plant seedlings, the plants whose leaf color is purple are fertile plants, and the non-purple plants are sterile plants. When transplanting, only the sterile plants are transplanted to the far...

Embodiment 2

[0024] A method for identifying the fertility of dual-purpose lines using leaf marker traits seedbed, the method is:

[0025] A. The GMS line was anti-A 3 MSC 1 and the restorer line J with chicken horn-type leaf markers 1 The dominant gene J controlling the horn-shaped leaves of chickens was used as a molecular marker.

[0026] B. On the basis of molecular markers, cloning of MSCs with dominant alleles of fertility control genes 1 and chicken horn-shaped leaf control dominant gene J, constructing the fertile dominant allele MSC of the fertility control gene 1 Linked Genome MSC Linked to Chicken Angle-Controlling Dominant Gene J 1 J.

[0027] C. Linking genomic MSCs 1 J was transformed into a high-yielding and high-quality cotton GMMS line to breed a GMMS line JA with chicken-horned leaf markers 1 (MSC 1 msc 1 Jj); the GMS line JA 1 Among the plant seedlings, the plant whose blade is chicken horn type is a fertile plant, and the plant whose blade is not chicken horn ...

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Abstract

The invention relates to a method for identification of dual-purpose line fertility by leaf marker property seedbed, comprising locking gene controlling cotton male nuclear fertility and gene controlling cotton marker properties( such as purple leaves, chicken horn shape leaves, etc.), by gene cloning techniques, transferring the genome to cotton nuclear sterile dual-purpose line, culturing the transgenic dual-purpose line with leaf marker properties. The fertile plants can be identified by the marker property during seedbed period and be pulled out. The plants transplanting to field are all sterile plants, so that there is no need to identify fertility at blooming period. The method is provided with greatly simplified preparation process, improved purity and reduced production cost of cotton hybrid seeds.

Description

Technical field [0001] The invention relates to a method for identifying the fertility of a cotton genital male sterile dual-purpose line, in particular to a method for identifying fertile plants of a hybrid cotton dual-purpose line at the seedbed stage and pulling them out in the seedbed. technical background [0002] Using heterosis is an effective way to greatly increase crop yield and improve quality. The research and utilization of cotton heterosis is at the leading level in the world. In the existing hybrid cotton seed production methods, manual detasseling requires manual removal of pollen, which is a large workload and high cost of seed production; the restorer line of the cytoplasmic sterile line is single , the yield advantage is not obvious, and it has not been widely used in production; the fertility stability of the "secondary method" using nuclear male sterile lines needs to be further studied, and it is only used on a small scale; only using nuclear male steri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00C12N15/65
Inventor 岳福良张相琼张小军李文均张小红周宏俊
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