Method for determining dual-purpose line fertility by use of leaf marking trait in seedbed stage

Abstract

The invention relates to a method for identification of dual-purpose line fertility by leaf marker property seedbed, comprising locking gene controlling cotton male nuclear fertility and gene controlling cotton marker properties( such as purple leaves, chicken horn shape leaves, etc.), by gene cloning techniques, transferring the genome to cotton nuclear sterile dual-purpose line, culturing the transgenic dual-purpose line with leaf marker properties. The fertile plants can be identified by the marker property during seedbed period and be pulled out. The plants transplanting to field are all sterile plants, so that there is no need to identify fertility at blooming period. The method is provided with greatly simplified preparation process, improved purity and reduced production cost of cotton hybrid seeds.

Description

Technical field [0001] The invention relates to a method for identifying the fertility of a cotton genital male sterile dual-purpose line, in particular to a method for identifying fertile plants of a hybrid cotton dual-purpose line at the seedbed stage and pulling them out in the seedbed. technical background [0002] Using heterosis is an effective way to greatly increase crop yield and improve quality. The research and utilization of cotton heterosis is at the leading level in the world. In the existing hybrid cotton seed production methods, manual detasseling requires manual removal of pollen, which is a large workload and high cost of seed production; the restorer line of the cytoplasmic sterile line is single , the yield advantage is not obvious, and it has not been widely used in production; the fertility stability of the "secondary method" using nuclear male sterile lines needs to be further studied, and it is only used on a small scale; only using nuclear male steri...

AI Technical Summary

Problems solved by technology

The research and utilization of cotton heterosis is at the leading level in the world. In the existing hybrid cotton seed production methods, manual detasseling requires manual removal of pollen, which is a large workload and high cost of seed production; the restorer line of the cytoplasmic sterile line is single , the yield advantage is not obvious, and it has not been widely used in production; the fertility stability of the "secondary method" using nuclear male sterile lines needs to be further studied, and it is only used on a small scale; only using nuclear male sterile lines The hybrid cotton prepared by the "two-line method" of the line is widely used (recently approved varieties include Chuanza 12, Chuanza 13, Chuanzamian 14, Chuanzamian 15, etc.)

However, because the dual-purpose lines for seed production need to be identified for fertility when they bloom, and the fertile plants are removed after the identification of fertility in the field flowering stage, the seed production procedure is complicated, labor-intensive and time-consuming, and it is difficult to popularize. The uneven distribution in the field will affect the yield of seed production, and the fertile plants will be pulled out after flowering, which will waste a lot of water and fertilizer resources and make the cost of seed production high