Cell organism microsystem for detecting cell surface marker and detection method thereof

Abstract

The invention relates to a cell micro system for detecting the mark material at the surface of cell and relative detecting method, wherein said system comprises a micro current control detecting chip, a light source, a convex lens, a lens, a camera, a computer, and a tested cell driver; said light source, convex lens, micro current control detecting chip and lens are arranged on the system base from up to down; the center of light source and the centers of convex lens, lens and reflective lens are in one line; said camera is connected to a computer; the computer is connected to the tested cell driver. Since the system comprises a micro current control detecting chip and the chip has micro current groove, the driving pump can pump sample cell into micro current groove to directly detect the mark material. And the system can avoid special external device; and the camera is connected to the computer, to online or off-line analyze the test process.

Description

Technical field [0001] The invention relates to an automated analysis system for detecting cell surface markers and a method for detecting cells with the analysis system. Background technique [0002] Cell surface markers are a class of protein molecules with important physiological significance that are expressed on the surface of cells and can usually be used to characterize specific cell types. At present, such surface markers have been widely used in basic research and clinical practice, such as the identification of cellular immune prototypes and the diagnosis of diseases. Flow cytometer is generally considered to be one of the most effective tools for detecting surface markers. It is a highly integrated automated analysis system that can perform rigorous quantitative analysis of markers. However, due to its expensive analysis costs (instrument prices, reagents and time consumption, etc.) and strict requirements for professional training of operators, this analysis technolog...

AI Technical Summary

Problems solved by technology

However, from the perspective of the detection and analysis process, the chip itself cannot independently realize the complete detection process, and the realization of the detection depends on many other devices besides the core component of the protein chip, and even usually expensive and large-scale external devices , such as ultra-clean equipment, various complex reaction signal detection equipment, etc.

In addition, from the perspective of cell surface markers, protein microarray chips generally need to purify proteins from cells, which not only increases time and cost, but also loses the physiological significance of markers to a certain extent

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