Plant lactobacillus and method of biological preparing conjugated linoleic acid using the same

A technology of conjugated linoleic acid and Lactobacillus plantarum, applied in the field of bioengineering, can solve problems such as being unable to be put into industrial applications, and achieve the effects of important commercial value and application prospects, good growth, and efficient production processes

Active Publication Date: 2007-03-14
JIANGNAN UNIV
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Problems solved by technology

[0007] Studies have shown that rumen bacteria such as Butyrivibrio fibrisolvens cannot be put into industrial application due to strict anaerobic conditions

Method used

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  • Plant lactobacillus and method of biological preparing conjugated linoleic acid using the same
  • Plant lactobacillus and method of biological preparing conjugated linoleic acid using the same

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1, the production process of conjugated linoleic acid biologically prepared by Lactobacillus plantarum CCTCC No.M206033

[0025] Effect of pre-culture time on CLA conversion rate:

[0026] Insert CCTCC No.M206033 into MRS medium (containing 0.5mg / ml LA or without LA) with 1% inoculum amount, collect the cells cultured at 37°C for 0, 6h, 12h, 18h and 24h respectively, and 2.0×10 10 The above cells were transferred to 1ml MRS (containing 1.0mg LA) at a final concentration of cfu / ml and cultured at 37°C for 24h. The CLA content was detected by gas chromatography. The results are shown in Figure 1.

[0027] It can be seen from Figure 1 that the ability of CCTCC No.M206033 cells to transform CLA through LA pre-culture is generally improved, especially in the cells at the end of the logarithmic growth phase (12h), the ability to transform CLA increases most significantly, and the average transformation of CLA The rate increased from 3.9 percent to 11.7 percent.

[...

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Abstract

The present invention is plant lactobacillus in the preservation number of CCTCC No. M206033 and the biological process of preparing conjugate linoleic acid with the lactobacillus, and belongs to the field of bioengineering technology. The plant lactobacillus can grow well in aerobic condition, and during fermentation with linoleic acid substrate contacting with the lactobacillus cell, linoleic acid will be converted into conjugate linoleic acid under the action of linoleic acid isomerase combined with membrane. The bioconversion process includes the first culturing of the lactobacillus strain in MRS culture medium containing 0.5mg/ml LA and the subsequent inoculation of the culture to MRS culture medium containing free linoleic acid in pH 6.5 at 37 deg.c with or without potassium phosphate buffering solution for culturing to produce conjugate linoleic acid.

Description

technical field [0001] Lactobacillus plantarum CCTCC No.M206033 and its biological preparation method of conjugated linoleic acid, especially a strain of Lactobacillus plantarum transformed from free linoleic acid to produce conjugated linoleic acid and the method of transformation production, belong to bioengineering technology field. Background technique [0002] Linoleic acid (abbreviated as LA) is an octadecadienoic acid with cis-9 and cis-12 conformations. Conjugated linoleic acid (CLA for short) is a general term for various linoleic acid isomers containing conjugated double bonds. It is the most important functional fatty acid discovered by humans in the past 20 years. Trans-10, cis-12 isomers are biologically active. Conjugated linoleic acid widely exists in various natural foods, mainly in milk fat and meat products of rumen animals such as cattle and sheep. The CLA content in each gram of milk fat ranges from 2 to 25 mg, and the CLA content varies with the milk f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/64C12R1/25
Inventor 陈卫张灏田丰伟赵建新丁虎生周凌华钮晓燕陈海琴
Owner JIANGNAN UNIV
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