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Method for fast breeding water caltrop seed and seedling

A technology of thorn grass and seedlings, which is applied in the field of rapid propagation of thorn grass seeds and seedlings, and achieves the effects of simple material source, low cost and easy planting.

Inactive Publication Date: 2007-04-11
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for rapid propagation of Smilax seedlings, which can effectively solve the problem of a large amount of supply and demand of Smilax seedlings. The materials are easy to obtain, the process is fast, not affected by seasons, and the cost is low.

Method used

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  • Method for fast breeding water caltrop seed and seedling
  • Method for fast breeding water caltrop seed and seedling
  • Method for fast breeding water caltrop seed and seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (A) collect healthy and robust Paleopsis seedlings from natural water bodies or in greenhouses in winter or spring;

[0042] (B) Rinse with tap water for 1-2 hours in the laboratory to remove the pollutants on the surface of the weed grass. After the weed grass seedlings are rinsed, remove the leaves and roots, cut the thick stems near the top, and rinse them with distilled water for 2-3 times. Wash the weed stems thoroughly;

[0043] (C) Sterilization and bud induction: tools such as plates, tweezers, and culture media that need to be used during the experiment must be sterilized by 0.1MPa high-pressure steam at 121°C, and the experimental tools should be placed in a biological safety cabinet or Sterilize in the sterile operating table for 30 minutes, and then use sterilizing agents to sterilize under aseptic conditions. The sterilizing agents are PPM-Plant preserve Mixture, ethanol, sodium hypochlorite and hydrogen peroxide. The sterilization method is to sterilize w...

Embodiment 2

[0074] Proliferation hormone ratio of A bud:

[0075] 2ip 2.5mg·L -1

[0076] B root inducing hormone ratio:

[0077] IBA 1.0mg·L -1 +2ip 0.5mg·L -1

[0078] Other implementation steps are identical with embodiment 1

Embodiment 3

[0080] Proliferation hormone ratio of A bud:

[0081] 6-BA 5.0mg·L -1

[0082] B root inducing hormone ratio:

[0083] NAA 1.0mg·L -1 +6-BA 0.5mg·L -1

[0084] Other implementation steps are the same as in Example 1.

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Abstract

The method for rapid propagation of water caltrop seedlings includes the following steps: firstly, collecting water caltrop plant from natural water body or greenhouse, making pretreatment and cleaning its surface, removing plant leaf and root, using the stem portion closed to top, sterilizing completely, cutting stem segment, placing the stem segment on the solid culture medium containing hormone to induce budding, transferring the bud with a certain length to propagation culture medium to make amplification culture, then making rooting culture and seedling naturalization, then planting the seedling in natural water body.

Description

technical field [0001] The present invention relates to the rapid propagation technology of Pachyrhiza seedlings, and also relates to a method for culturing the stem node tissue of Pachyrhiza. It is suitable for a large number of seedling needs of aquatic vegetation restoration projects, and can also provide seedlings for aquatic garden landscapes, ornamental aquatic plants, and aseptic seedlings for the research of weed. Background technique [0002] In recent years, with the implementation of the national sustainable development strategy and the improvement of the level of social development and the quality of people's life, water pollution control and improvement of water environment quality have attracted the attention of the whole society. Both national and local governments have formulated relevant plans, or carried out relevant scientific research and engineering projects. For example, during the "Tenth Five-Year Plan" period, the Ministry ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 杨劭高健刘治华
Owner HUAZHONG NORMAL UNIV
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