Immune response stimulating composition comprising nanoparticles based on a methyl vinyl ether-maleic acid copolymer

一种甲基乙烯基醚、纳米颗粒的技术，应用在包含基于甲基乙烯基醚－马来酸酐共聚物的纳米颗粒的刺激免疫反应的组合物领域，能够解决免疫原或变应原活性成分剂量高等问题

Active Publication Date: 2007-06-06
UNIV DE NAVARRA
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As is known, despite its potential advantages, oral immunization for therapeutic or prophylactic purposes must overcoming several obstacles

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immune response stimulating composition comprising nanoparticles based on a methyl vinyl ether-maleic acid copolymer
  • Immune response stimulating composition comprising nanoparticles based on a methyl vinyl ether-maleic acid copolymer
  • Immune response stimulating composition comprising nanoparticles based on a methyl vinyl ether-maleic acid copolymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] immunity therapy

[0151] Preparation and Characterization of Ovalbumin-Based Methyl Vinyl Ether-Maleic Anhydride (PVM / MA) Copolymer Nanoparticles

[0152] The protein chosen was ovalbumin (OVA) as it is currently widely used as an experimental model for allergies.

[0153] Ovalbumin accounts for more than 50% of the protein content in egg whites. It is a monomeric phosphorylated glycoprotein with a molecular weight of 43 to 45 kDa and 385 amino acids [Johnsen and Elsayed, Mol Immunol, 27 (1990) 821]. Ovalbumin is the egg protein with the strongest allergenic ability, which immediately induces a type I hypersensitivity reaction mediated by IgE.

[0154] The methods described below can be used to efficiently prepare nanoparticle-based colloidal dosage forms for immunotherapy.

[0155] 1.1 Preparation of empty nanoparticles (NP)

[0156] 100 mg of methyl vinyl ether-maleic anhydride copolymer (PVM / MA) [Gantrez_AN 119] was dissolved in 5 mL of acetone. Next, 10 mL of ...

Embodiment 2

[0192] In vitro testing of release of ovalbumin from nanoparticles

[0193] To assess the release of ovalbumin in vitro, nanoparticles were incubated in 1 mL of PBS (phosphate buffered saline, pH 7.4) at a concentration of approximately 8 mg / mL in "eppendorf" tubes. Tubes were incubated in an oven at 37 °C under rotation, and samples were centrifuged at 26,500 x g for 20 min at predetermined time intervals, and supernatants were collected for subsequent analysis. The released ovalbumin was detected by the bicinchoninic acid method.

[0194] The obtained release profile of ovalbumin is represented in Figure 1; it was observed that ovalbumin was faster in formulation NP I than in formulations NP II, NP III and NP IV which exhibited similar release profiles. This is because in formulation NP I, ovalbumin is adsorbed on the outer surface of the nanoparticles, so the initial release (burst) is slower than in other formulations (NP II, III and IV) where ovalbumin is partially encap...

Embodiment 3

[0196] Quantification of anti-OVA antibodies produced after immunization with ovalbumin in BALB / c mice

[0197] 65 BALB / c mice were immunized, and they were divided into 13 groups according to the method of administration.

[0198] The controls used were ovalbumin-free solution (OVA) (10 μg intradermally, 25 μg orally) and empty nanoparticles (NP) (intradermally and orally), as well as ovalbumin adsorbed to aluminum hydrogel. Albumin (OVA-Alum) (10 μg administered intradermally) served as a positive control for inducing a Th2 response characterized by high IgG 1 Potency [Faquim-Mauro et al., Int Immunol, 12 (2000) 1733-1740].

[0199] The remaining groups were vaccinated intradermally (10 μg OVA) or orally (25 μg OVA), and the treatments were:

[0200] a) intradermal inoculation of ovalbumin (OVA) solution

[0201] b) Oral inoculation with ovalbumin (OVA) solution

[0202] c) Intradermal inoculation of ovalbumin (OVA-Alum) adsorbed on aluminum hydrogel

[0203] d) Intrade...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The composition for stimulating an immune response in a subject comprises methyl vinyl ether and maleic anhydride copolymer-based nanoparticles. Said nanoparticles may further contain an allergen or an antigen and / or an immunostimulating agent, which may be contained inside said nanoparticles and / or at least partially coating the surface of said nanoparticles, and optionally a cross-linking agent. The immune response stimulating composition is useful as an adjuvant in immunotherapy and vaccines.

Description

field of invention [0001] The present invention relates to the use of nanoparticles based on methyl vinyl ether-maleic anhydride copolymers as adjuvants in immunotherapy and vaccines, optionally containing allergens or antigens and / or immunostimulants. The invention also relates to compositions for stimulating an immune response comprising said nanoparticles. Background of the invention [0002] It is known that there are highly immunogenic antigens capable of inducing a protective immune response in an individual, whereas there are other antigens which do not induce said protective immune response, or which induce a very weak immune response. Typically, a host immune response to a weakly immunogenic antigen can be stimulated by the co-administration of an adjuvant. [0003] Adjuvant [0004] An adjuvant is any substance that increases the immune response to the antigen with which it is mixed. Adjuvants function mainly through three mechanisms: i) forming antigen or aller...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K39/112A61K39/39
CPCB82Y5/00A61K36/06A61K2039/55594A61K39/39A61K2201/103A61K2039/55555A61K2039/55572A61K9/14A61K36/00A61K39/0275A61K51/1244Y10S977/773A61P37/04Y02A50/30A61K2300/00A61K9/51A61K2039/55511
Inventor 胡安马奴埃尔·伊拉切尔贾立塔卡洛斯·加马左德拉拉斯拉马力亚路易萨·圣拉如加马塔·皮尔布加布特赫兹·圣朗漫阿贝拉斯度里赫斯曼H·A·沙尔曼萨拉·戈梅斯马迪内加维尔·欧撤尔赫巴哈兹
Owner UNIV DE NAVARRA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap