Neuroprotective composition and uses thereof

Abstract

A neuroprotective composition for protecting neuronal cells against oxidative stress and methods for using and preparing the same. More particularly, the neuroprotective composition of the invention comprises a mixture of pyruvate, antioxidant, and lipid(s) such as fatty acids. The neuroprotective composition could be used for the treatment of brain trauma, brain or cerebrovascular ischemia, neurodegenerative diseases, poisoning of neuronal cells, the diminution of drugs side effects and for preservation of neuronal grafts.

Description

[0001] 1. Field of the Invention[0002] The present invention relates to the use of an amphiphilic antioxidant composition as a neuroprotective agent and to methods for using and preparing the same. More particularly, the present invention pertains to the use of a formulation of pyruvate, antioxidant, and lipid(s) such as fatty acids for protecting neurons against oxidative stress.[0003] 2. Description of the Prior Art[0004] Reactive oxygen species (ROS) have been implicated in the development of many heart and brain dysfunctions. Ischemia / reperfusion insults to these organs are among the leading causes of mortality in America. These insults are caused by complete or partial local occlusions of heart and brain vasculature, by heart stroke or attack, and by cerebral attacks and trauma to the brain. In addition, ROS are involved in artherosclerotic lesions, in the evolution of various neurodegenerative diseases, and are also produced in association to epileptic episodes, in inflammatio...

AI Technical Summary

Benefits of technology

[0022] An advantage of the present invention is that it provides effective means for preventing the loss of viability or functions of neuronal cells in conditions of oxidative stress. It can also protect a neuronal cell from a toxic substance, stabilizes the cellular membrane of a neuronal cell and / or helps in the normalization of neuronal cellular functions.

[0023] Other objects and advantages of the present invention will be apparent upon reading the following non-restrictive description of several preferred embodiments made with reference to the accompanying drawings.

[0024] FIG. 1 is a graph showing the in vitro ROS production by peroxide-based prooxidant systems used with P19 neurons.

[0025] FIG. 2 is a graph showing the protection provided by TRIAD to P19 neurons exposed for different times to XA / XAO mediated oxidative stress.

[0026] FIG. 3 is a graph showing the protection provided by TRIAD components to P19 neurons exposed for different times to XA / XAO mediated oxidative stress.

[0027] FIG. 4 is a graph showing the protection provided by TRIAD to P19 neurons exposed to oxidative stress mediated by different concentrations of XAO.

Problems solved by technology

Until now, no ideal therapeutic agent is known to protect neuronal cells against oxidant species associated with various types of oxidative stress.

However, none of these patents discloses or suggests the use of TRIAD as neuroprotective agent.

Indeed, deleterious action of various toxicants and drugs is exerted via production of ROS.

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