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Methods of treating cancer using IL-21

a cancer and il-21 technology, applied in the field of cancer treatment methods using il21, can solve the problems of not being cured, not being able to be treated as easily,

Inactive Publication Date: 2007-03-01
ZYMOGENETICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a method of treating Non-Hodgskins lymphoma and cancer (such as renal cell carcinoma, epithelial carcinoma, breast cancer, prostate cancer, ovarian cancer and colon cancer) by administering a polypeptide or a fusion protein with a functional activity of IL-21. The polypeptide or fusion protein has been shown to not cause proliferation of isolated cancer cells prior to administration to the subject. The treatment results in a tumor response, which can be measured as complete response, partial response or reduction in time to progression.

Problems solved by technology

Chemotherapy is usually more effective in treating aggressive lymphoma, whereas indolent lymphomas cannot be treated as easily and therefore are likely never to be cured as long as the disease remains indolent.

Method used

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  • Methods of treating cancer using IL-21

Examples

Experimental program
Comparison scheme
Effect test

example 1

Mouse IL-21 is Active in Mouse Bone Marrow Assay

A. Isolation of Non-Adherent Low Density Marrow Cells:

[0130] Fresh mouse femur aspirate (marrow) was obtained from 6-10 week old male Balb / C or C57BL / 6 mice. The marrow was then washed with RPMI+10% FBS (JRH, Lenexa Kans.; Hyclone, Logan Utah) and suspended in RPMI+10% FBS as a whole marrow cell suspension. The whole marrow cell suspension was then subjected to a density gradient (Nycoprep, 1.077, Animal; Gibco BRL) to enrich for low density, mostly mononuclear, cells as follows: The whole marrow cell suspension (About 8 ml) was carefully pipetted on top of about 5 ml Nycoprep gradient solution in a 15 ml conical tube, and then centrifuged at 600×g for 20 minutes. The interface layer, containing the low density mononuclear cells, was then removed, washed with excess RPMI+10% FBS, and pelleted by centrifugation at 400×g for 5-10 minutes. This pellet was resuspended in RPMI+10% FBS and plated in a T-75 flask at approximately 106 cell...

example 2

IL-21 Transzenic Mice

A. Generation of Transgenic Mice Expressing Human and Mouse IL-21

[0134] DNA fragments from transgenic vectors (U.S. Pat. No. 6,307,024) containing 5′ and 3′ flanking sequences of the respective promoter (MT-1 liver-specific promoter (mouse IL-21 (U.S. Pat. No. 6,307,024) or lymphoid specific LCK promoter (mouse and human IL-21 (U.S. Pat. No. 6,307,024), the rat insulin II intron, IL-21 cDNA and the human growth hormone poly A sequence were prepared and used for microinjection into fertilized B6C3fl (Taconic, Germantown, N.Y.) murine oocytes, using a standard microinjection protocol. See, Hogan, B. et al., Manipulating the Mouse Embryo. A Laboratory Manual Cold Spring Harbor Laboratory Press, 1994.

[0135] Eight transgenic mice expressing human IL-21 from the lymphoid-specific EμLCK promoter were identified among 44 pups. Four of these were pups that died and 4 grew to adulthood. Expression levels were fairly low in these animals. Twenty transgenic mice expres...

example 3

IL-21 Purified Recombinant Human Protein

Dose-Response Study in Normal Mice

A. Summary

[0142] Normal six week old female C57B1 / 6 (Harlan Sprague Dawley, Indianapolis, Ind.). mice were treated by intraperitoneal injection once daily for either four or eight days with one of four dose levels of purified recombinant human IL-21 (U.S. Pat. No. 6,307,024) at 0.1, 0.5, 5 or 50 μg / mouse / day or with vehicle as a control. Body weights and body temperatures were monitored daily. On either day four or day nine, four of the eight mice from each protein treatment group and five of the ten mice in the vehicle control group were sacrificed. Blood, bone marrow and tissues were harvested and analyzed. Potential perturbations in lymphoid tissues were examined, as well as general physiologic and toxicological parameters.

[0143] There was no evidence of toxicity of human IL-21 protein at any of the doses tested. Body weights and temperatures were unchanged. There were no apparent changes in clinical...

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Abstract

Methods for treating mammals with cancer using molecules that have an IL-21 functional activity are described. The molecules having IL-21 functional activities include polypeptides that have homology to the human IL-21 polypeptide sequence and proteins fused to a polypeptide with IL-21 functional activity. The molecules can be used as a monotherapy or in combination with other known cancer therapeutics.

Description

REFERENCE TO RELATED APPLICATIONS [0001] This patent application is a divisional of U.S. patent application Ser. No. 10 / 456,780, filed on Jun. 6, 2003, and is a continuation-in-part which claims benefit of U.S. Provisional Application Ser. No. 60 / 387,127, filed on Jun. 7, 2002, and all of which are incorporated by reference herein.BACKGROUND OF THE INVENTION [0002] Cytokines generally stimulate proliferation or differentiation of cells of the hematopoietic lineage or participate in the immune and inflammatory response mechanisms of the body. Examples of cytokines which affect hematopoiesis are erythropoietin (EPO), which stimulates the development of red blood cells; thrombopoietin (TPO), which stimulates development of cells of the megakaryocyte lineage; and granulocyte-colony stimulating factor (G-CSF), which stimulates development of neutrophils. These cytokines are useful in restoring normal blood cell levels in patients suffering from anemia, thrombocytopenia, and neutropenia o...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/20A61K38/21C07K14/54
CPCA61K38/193A61K38/20A61K38/2013A61K38/2026C07K2317/73A61K45/06C07K14/54C07K16/2878A61K38/2086A61P1/00A61P1/04A61P1/16A61P11/00A61P19/02A61P25/00A61P31/00A61P31/04A61P31/06A61P31/10A61P31/12A61P31/14A61P31/16A61P31/18A61P31/20A61P31/22A61P33/02A61P35/00A61P35/02A61P35/04A61P37/02A61P37/04A61P9/00Y02A50/30
Inventor NELSON, ANDREW J.HUGHES, STEVEN D.HOLLY, RICHARD D.
Owner ZYMOGENETICS INC
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