Method of physicochemically producing glycogen and glycogen obtained by the same
a physicochemical and glycogen technology, applied in the field of physicochemically producing glycogen and glycogen obtained by the same, can solve the problems of complex steps, difficult chemically synthesizing polysaccharide sugar chains, and no known technique for synthesis of sugar chains, and achieve the effect of simple operations
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example 1
Preparation of Panax Notoginseng Extract
[0057]91 kg of granules (2 to 3 mmφ) of Panax notoginseng, 9 kg of citric acid and 550 L of water were placed in a tank with a pressure device, followed by heating at 90 to 96° C. for one hour. Thereafter, the mixture was kept under a pressure of 1.1 kgf / cm2 for one hour. The resultant reaction solution was subjected to filtration using a pressure filtration apparatus having NA500 filter paper. The resultant extract solution was aliquoted. Thereafter, 700 L of 45 wt % ethanol solution was added to the Panax notoginseng extract residue, followed by heating with reflux at 65° C. for 2 hours. The resultant reaction solution was subjected to filtration using a pressure filtration apparatus having NA500 filter paper and the ethanol extract solution was aliquoted. The thus-obtained citric acid extract solution and ethanol extract solution were mixed together and condensed. The resultant condensed solution was subjected to spray drying, resulting in ...
example 2
Study on Conditions for Production of Glycogen
[0060]175 mL of water, 30 to 35 g of Panax notoginseng unprocessed tissue, and citric acid corresponding to 9 wt % of the Panax notoginseng unprocessed tissue were added to each of four Erlenmeyer flask, which were in turn covered with food wrapping film, followed by boiling under a pressure of 1.1 to 1.2 kgf / cm2 for 10 min, 20 min, 35 min, and 60 min, respectively, in an autoclave. Each reaction product was allowed to cool. The solution portion of the reaction product was centrifuged (3,000 rpm, 5 min) to obtain an extract solution. Precipitation residue was returned to the Erlenmeyer flask still containing the original Panax notoginseng unprocessed tissue. 200 mL of 50% ethanol solution was added to the flask, which was in turn fitted with a reflux condenser, and the solution was heated at 85° C. for 1 to 1.5 hours. The resultant heated solution was allowed to cool, followed by centrifugation (5,000 rpm, 10 min). The ethanol extraction...
example 3
[0062]Glycogen production rates were compared by varying citric acid concentration under the pressure conditions obtained in Example 2 (i.e., 1.1 to 1.2 kgf / cm2, 35 min). The same procedure as that of Example 2 was carried out, except that the citric acid concentration was changed to 5, 6, 7, 8 and 9% relative to the material. The results are shown in Table 2. As shown in Table 2, in the case of the 9% citric acid addition, the glycogen production rate was the highest 41.4%. Next, glycogen production rates were compared by varying pressure time.
TABLE 2Dependence of glycogen production on citric acid concentrationCitric acid additionconcentration relative toGlycogen production rateExtract amountmaterial(%)(%)9%41.474.08%38.372.37%35.668.96%29.166.65%30.963.2
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