Antibacterial coatings that inhibit biofilm formation on implants

a biofilm and antibacterial technology, applied in the field of biomedical implants, can solve the problems of non-biodegradable or degradable materials, and achieve the effects of stable osteotomy, less inflammation, and reduced healing tim

Inactive Publication Date: 2015-11-19
THE TRUSTEES OF THE UNIV OF PENNSYLVANIA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The coating effectively inhibits biofilm formation, reduces healing times, and decreases inflammation, as demonstrated in large animal models, with sustained antimicrobial activity and no adverse impact on tissue physiology, thereby addressing the challenges of implant-associated infections.

Problems solved by technology

The material may be non-biodegradable or may degrade after a certain period of time.

Method used

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  • Antibacterial coatings that inhibit biofilm formation on implants
  • Antibacterial coatings that inhibit biofilm formation on implants
  • Antibacterial coatings that inhibit biofilm formation on implants

Examples

Experimental program
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Effect test

example 1

In Vitro Challenge Assay

[0150]Metallic Orthopedic Pins

[0151]To scale-up and validate the aforementioned coating procedure for orthopedic hardware, two different metals were used: (1) commercially pure Titanium (cpTi) and (2) stainless steel 316L. Bone plate chips measuring approximately 1 cm by 1 cm were cut transversely from orthopedic bone plates (Locking Compression Plates (LCP); Synthes, West Chester, Pa.) using a diamond saw (EXAKT System, Norderstedt, Germany). They were divided into two groups: control (no surface modification) and treatment (coated with N,N-dodecyl,methyl-PEI) groups, with all assays run in triplicate.

[0152]For the bacterial challenge assay, Staphylococcus aureus (ATCC 25923; this strain of S. aureus is susceptible to commonly used antimicrobials) was cultured in trypticase soy broth (TSB) with shaking at 37° C. for 16 h. A 1-mL aliquot of this culture was sub-cultured with 9 mL of fresh TSB for an additional 2 h prior to dilution to 0.5 McFarland Standard t...

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Abstract

Orthopedic and dental implants coated with an antibacterial coating and methods of making and using, are described herein. The implant can be coated with a hydrophobic, polycationic antibacterial polymer. The polymer can be covalently or non-covalently associated with the surface; however, in particular embodiments, the polymer is non-covalently associated with the surface. As shown in the examples below, clinical observations, digital radiography, and a battery of well-accepted ex vivo analytical methods show that the presence of a hydrophobic polycationic polymer coating, such as N,N-dodecyl,methyl-PEI coating on the surface of a metal implant, was effective in eliminating the clinical signs of infection in vivo in a large animal infection model. Moreover, the coated plates supported bone healing, and in fact decreased healing times, even in the presence of significant bacterial contamination compared to a control.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of copending application Ser. No. 13 / 647,107, filed Oct. 8, 2012, which was a continuation-in-part (CIP) of application Ser. No. 11 / 107,412, filed Apr. 14, 2005, which was a continuation of application Ser. No. 10 / 123,860, filed Apr. 15, 2002, which claims priority to U.S. Ser. No. 60 / 285,883, filed Apr. 23, 2001; U.S. Ser. No. 60 / 340,078, filed Dec. 10, 2001; and U.S. Ser. No. 60 / 368,495, filed Mar. 29, 2002, and also claims priority to Application Ser. No. 61 / 543,981, filed on Oct. 6, 2011, the entire contents of which are hereby incorporated by reference.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]This invention was made with Government Support under Contract No. W911NF-07-D-0004, awarded to Massachusetts Institute of Technology by the Army Research Office. The Government has certain rights in the invention.FIELD OF THE INVENTION[0003]This invention is in the field of biomedic...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61L31/16A61L31/10A61L27/34A61L29/08A61L27/54A61L29/16
CPCA61L31/16A61L27/54A61L29/16A61L27/34A61L2420/02A61L31/10A61L2300/404A61L2300/606A61L29/085A01N33/12A61L2430/14A01N25/10A01N25/34C08L79/02C08L71/02A61F2/02A61F2/14A61L27/50C08L33/14
InventorSCHAER, THOMAS P.STEWART, SUZANNEKLIBANOV, ALEXANDER M.
OwnerTHE TRUSTEES OF THE UNIV OF PENNSYLVANIA