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Composition and method for generating a desired cell type and/or tissue type from hair follicular stem cells

a technology of hair follicle stem cells and cell types, applied in the field of tissue engineering, can solve the problems of high cost, high ethical concerns, and inability to naturally differentiate into any type of cells, and achieve the effects of reducing the cost of induced pluripotent stem cells, reducing the number of cells, and increasing the number of cells

Inactive Publication Date: 2015-12-17
GHO CONRADUS GHOSAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes a composition that can be used to generate new cells or tissues for transplantation into a recipient subject. The composition includes a physiologically acceptable vanadium compound, particularly bis (maltolato) oxovanadium, which has been found to be more effective than other anti-apoptotic agents. The use of this compound boosts the efficiency of other ingredients present in the composition. Additionally, the patent suggests that using stem cells or components of the composition derived from the same recipient subject can further enhance the compatibility of the transplant and reduce the risk of complications related to immune rejection or infection.

Problems solved by technology

The procurement of such cells is, however, highly limited and constantly subjected to severe ethical concerns since natural pluripotent stem cells are from embryonic origin and thus can only be obtained from an embryo.
In other words, non-embryonic stem cells cannot naturally differentiate into any type of cells, at least not without undergoing artificial manipulations.
However, the use of induced pluripotent stem cells is controversial since such cells are often highly tumorigenic.
Serum is a major source of viral contaminants which, once present, are difficult to remove from cultures.
It can contain viruses, prions and mycoplasma, which may skew the outcome of scientific experiments and may transfer diseases to cultured cell.
Additionally, considerable ethical debate surrounds the production of Foetal Bovine Serum.

Method used

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  • Composition and method for generating a desired cell type and/or tissue type from hair follicular stem cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

Collection of Hair Follicular Stem Cells

[0113]Hair follicular stem cells were obtained from plucked hairs in the anagen phase, which were obtained from a donor subject, using a plucking instrument such as a hollow harvesting needle. Plucked hairs were inspected under a microscope. Plucked hairs not displaying the characteristics of a hair in the anagen phase were discarded.

example 2

Pre-Treatment of Hair Follicular Stem Cells

[0114]Plucked hairs in the anagen phase were immersed in 1% collagenase type IV for 2 hours at 37° C. in order to enzymatically dissociate the hair follicular stem cells from the hair follicle. Hair follicular stem cells were then rinsed several times, and resuspended in culture medium.

example 3

Collection and Culture of CD34-Positive Cells

[0115]CD34-positive cells act as “circulating fibrocytes” and their function is dependent on the environment. For instance, in wound healing CD34-positive cells concentrate around the damaged tissue. In the context of tissue generation, CD34-positive cells act as a stem cell enhancer. Specifically, when hair follicular stem cells are cultured together with CD34-positive cells, hair follicular stem cells are able to proliferate without supplement.

[0116]CD34-positive cells were obtained from the peripheral circulation of a donor subject. CD34-positive cells were isolated from the blood using a MACS cell separation kit (Miltenyl Biotec). Isolated CD34-positive cells were then rinsed several time, resuspended in the culture medium (RPMI 1640, GIBCO, Invitrogen) containing autologous serum, and cultured in the same medium for a period of 8 weeks.

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Abstract

The present invention is concerned with a composition and in vitro method for generating a desired cell type and / or tissue type from hair follicular stem cells. The composition and in vitro method are particularly suitable for generating an autologous desired cell type and / or tissue type. Furthermore, the composition and method are especially efficient and suitable for use in the context of cosmetic cell and / or tissue transplantation in recipient areas of a subject experiencing cell and / or tissue loss caused by, for example, a wound, scar, burn injury, tissue degeneration, and aging. The composition and in vitro method are also suitable to circumvent complications related to infections and / or immune rejection of a cosmetic cell and / or tissue implant or graft.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is the National Stage of International Application No. PCT / NL2014 / 050062 filed Feb. 3, 2014, which claims the benefit of Netherlands Application No. NL 2010222, filed Feb. 1, 2013, the contents of which is incorporated by reference herein.FIELD OF THE INVENTION[0002]The present invention is in the fields of tissue engineering with applications in the fields of cosmetic and aesthetic procedures and regenerative medicine. The present invention provides an improved composition and in vitro method for generating a desired cell type and / or tissue type from at least one hair follicular stem cell. The improved composition and in vitro method of the present invention are particularly suitable for cosmetic or therapeutic cell and / or tissue transplantation in recipient areas of a subject experiencing cell and / or tissue loss caused by a wound, scar, burn injury, tissue degeneration, diseases and / or aging. The composition and in vitr...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/071A61K35/12
CPCC12N5/0602C12N2506/03A61K35/12C12N5/0628C12N5/0666C12N2500/20C12N2500/24C12N2500/38C12N2500/40C12N2500/44C12N2501/11C12N2501/115C12N2501/13C12N2501/14C12N2501/999C12N2502/11C12N2502/115C12N2509/00
Inventor GHO, CONRADUS GHOSAL
Owner GHO CONRADUS GHOSAL
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