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Oil of microorganisms rich in docosahexaenoic acid

Pending Publication Date: 2022-02-10
FERMENTALG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides a DHA-rich oil that can be used in smaller amounts in mixtures and compositions. This oil can also be obtained from biomass. It has technical advantages because it can be used in smaller amounts, making it more efficient and cost-effective.

Problems solved by technology

Consequently, the concentrated oil is more vulnerable to oxidation.
However, this concentrated oil is rather different from the natural oil, it has undergone a number of transformation processes that have changed its composition: fatty acid profile, vitamins, pigments and other antioxidant molecules, depriving the PUFAs of their natural protection.
There is at present little data available concerning the impact of concentration processes on the formation of contaminants.
However, the re-formation of triglycerides from ethyl esters may lead to an increase in diglycerides, which are contaminant precursor compounds.
Moreover, these concentrated oils are generally obtained by treatment processes which are expensive and harmful to the environment.
However, the choice of technical solutions is limited by the use made of the oils obtained, in particular in human food (Fedorova-Dahms I.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

d Profile of High DHA Content Biomass of Thraustochytrids

[0082]Strains of thraustochytrids (Aurantiochytrium mangrovei—FCCB1897, FCCB1800, CCAP4062 / 8) are grown in Erlenmeyer flasks in ATCC 790 (modified) culture medium. Similar results are obtained with strains of Schizochytrium sp. (in particular with strain CCAP4087 / 7).

[0083]Once the cultures are in the stationary phase, the biomass is recovered by centrifugation and then freeze-dried before analysis of the fatty acid composition of the biomass by GC-FID (method adapted from standard ISO 12966-2).

[0084]Composition of the Modified ATCC 790 Medium:

Yeast extract5.0 g / LPeptone5.0 g / LD+-Glucose30.0 g / L Sea salts 20 g / L

[0085]Table 1 represents the composition of fatty acids contained in the biomass. The results are expressed as a percentage of the total fatty acid content. SFAs are saturated fatty acids.

TABLE 1FCCB1897FCCB1800CCAP4062 / 8C10:00.00.00.0C11:00.00.00.0C12:00.00.00.0C13:00.00.00.0C14:00.20.30.3C14:10.00.00.0C15:00.61.01.7C15...

example 2

Cultures of High DHA Content Strains

[0086]The cultures are carried out in fermenters (bioreactors) from 1 to 5 L useful with dedicated automated systems and supervision by computer station. They are carried out using two strains of Aurantiochytrium mangrovei and with two different culture protocols. The system is regulated at pH 5 via the addition of base (NH4OH for example b1 and b2 and with NaOH for example a) with pH adjustment carried out throughout the culture period, and providing a nitrogen supply (in the context of examples b1 and b2). The culture temperature was set at 30° C. then 22° C. and finally 18° C. at the end of the culture.

[0087]Strain CCAP4062 / 7 is used for example a and b1 whereas strain FCCB1897 is used for example b2.

[0088]The composition of the culture media is given in Table 2.

TABLE 2ab1 and b2CaCl2, 2H2O0.550.55g / LMgSO4, 7H2O4-84-8g / LH3B030.00875-0.175 0.00875-0.0175 g / LK2SO42.080.00g / LKH2PO44.004.00g / LNa4EDTA, 2H2O0.120.12g / LFeSO4, 7H2O0.040.04g / L(NH4)2SO49...

example 3

nder Industrial Conditions

[0093]High DHA content strains produce a biomass with a similar fatty acid composition when grown in industrial size fermenters, such as 10 m3 or 180 m3 tanks, under conditions similar to example 2, with culture medium b and glucose additions in the form of an enrichment solution are made with a carbon:nitrogen:phosphorus (CNP) molar ratio of 533:0.4:1.

[0094]The fatty acid profiles of the CCAP4062 / 7 strain biomass after culture in 10 m3 and 180 m3 tanks are given in Table 4. The results are expressed as a percentage of the total fatty acid content.

TABLE 410 m3180 m3C10:00.00.0C11:00.00.0C12:00.00.0C13:00.00.0C14:00.70.5C14:10.00.0C15:00.00.0C15:10.00.0C16:018.113.6C16:10.00.0C16:20.00.0C16:30.00.0C16:40.00.0C17:00.00.0C17:10.00.0C18:00.80.0C18:10.00.0C18:20.00.0C18:3n30.30.3C18:3n60.00.0C18:4n30.00.0C20:00.00.0C20:4n6 (ARA)0.20.1C20:5n3 (EPA)0.90.4C21:00.00.0C22:00.00.0C22:5n3 (DPAn3)0.00.0C22:5n6 (DPAn6)11.213.4C22:6n3 (DHA)63.966.8DHA + DPA75.180.2SFA19.6...

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Abstract

The present invention relates to an oil of microorganisms rich in docosahexaenoic acid (DHA, C22:6n3), comprising more than 60% of DHA relative to the total mass of fat and to the use thereof for human or animal feed, in particular for feeding infants, children, or pregnant or lactating women.

Description

FIELD OF THE INVENTION[0001]The present invention relates to an oil of microorganisms rich in docosahexaenoic acid (DHA, C22:6n3), comprising more than 60% DHA in relation to the total mass of fat and at least 80% triglycerides in relation to the total mass of fat.STATE OF THE ART[0002]Oils containing DHA come from several sources, the most well known are fish, krill and microorganisms such as microalgae. Many strains of microorganisms are known to produce PUFAs, in particular docosahexaenoic acid (DHA), arachidonic acid (ARA) or eicosapentaenoic acid (EPA), also identified by the signs co 3 and o6. These PUFAs are widely used in industry, in particular for human or animal food, or in cosmetics, and their industrial production has been continuously improved for many years (WO 1997 / 037032, WO 2001 / 054510, WO 2013 / 136028, WO 2015 / 004402, US 2017 / 016036, US 2017 / 335356). The criteria for selecting strains suitable for industrial production are their high biomass productivity, a signifi...

Claims

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Application Information

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IPC IPC(8): C12P7/64A23L33/115C12N1/10
CPCC12P7/6472C12R2001/90C12N1/105A23L33/115A23K20/158C11C1/002A23V2002/00A23V2250/186A23V2250/1868A23V2250/194
Inventor GODART, FRANCOISLAPENDRIE, ADELINE
Owner FERMENTALG