The invention discloses dedicated environment-friendly fertilizer for pine berries and a preparation method of the dedicated environment-friendly fertilizer. The dedicated environment-friendly fertilizer for the pine berries is prepared from the following raw materials: sheep bone meal, leaf mold, plant ash, guano, banana peels, fresh lotus leaves, caulis benincasae, rhizome typha orientalis, EM fungicide, humic acid, urea, disodium hydrogen phosphate, potassium nitrate, copper sulfate, magnesium sulfate, ammonium molybdate, soil improver and water. The fertilizer provided by the invention comprises inorganic fertilizer, microbial fungicide and organic fertilizer, so that the nutrients of the fertilizer are completed, the absorption and conversion of pine berry plants on the fertilizer efficiency are promoted, the fertilizer utilization efficiency is improved, the soil quality is improved, the soil fertility is improved, the environmental pollution caused by the fertilizer is reduced, the production cost is low, the process is simple, the use is convenient, and the economic and environment benefits are good.