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Method for extractiong microbicide and anti-staling agent from tupistra ker, and its application

An open arrow and extract technology, applied in the field of pesticides, can solve the problems of environmental pollution, excessive pesticide residues in agricultural products, etc., and achieve the effect of broad-spectrum antibacterial activity

Inactive Publication Date: 2007-11-14
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of agriculture, the abuse of chemically synthesized pesticides and their own shortcomings are the main factors leading to resistance to pests and diseases, excessive pesticide residues in agricultural products and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 Aquamarine extract

[0021] Crush the dried open arrow rhizome or whole grass, and pass through a 40-60 mesh sieve. According to the ratio of open arrow pulverized matter: water = 1:3~8 (weight / volume), extract for more than 24 hours, filter to obtain a part of the extract, centrifuge and dry the residue to obtain another part of the extract and filter, combine the two parts of the extract, Concentrate in vacuo to a concentrated liquid equal in weight to the crushed product of the open arrow or dry to obtain a solid extract, which is stored at low temperature for use.

Embodiment 2

[0022] Example 2 Preparation of Open Arrow Organic Solvent Extract

[0023] Crush the dried open arrow rhizome or whole grass, and pass through a 40-60 mesh sieve. According to the ratio of the crushed product of the open arrow: methanol = 1:3~5 (weight / volume), place it in a reflux extraction device for heating and extraction, filter to obtain a part of the extract, centrifuge and dry the residue to obtain another part of the extract, filter it, and combine the two parts The extract is then vacuum concentrated to obtain a concentrated solution equal to the crushed product of the open arrow or dried to obtain a solid extract, which is stored at low temperature for future use.

Embodiment 3

[0024] Example 3 Preparation of Fractional Extract from Open Arrow Solvent

[0025] Put the water extract or organic solvent extract in the liquid separation device, add 5-10 times (weight / volume) of chloroform, stir well and let it stand, after the mixed solution is clarified and separated, take out the chloroform layer, Concentrate in vacuo to obtain chloroform extract, store at low temperature for later use; add 5-10 times (weight / volume) ethyl acetate to the remaining part after chloroform extraction, stir well and let it stand, after the mixed solution is clarified and stratified, take out the acetic acid The ethyl acetate layer was concentrated in vacuo to obtain the ethyl acetate extract, which was stored at low temperature for later use; the remaining part after the ethyl acetate extraction was added with 5-10 times (weight / volume) n-butanol, fully stirred and left to stand until mixed After the liquid was clarified and separated, the n-butanol layer was taken out and ...

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Abstract

A process for extracting the bactericide or antistaling agent from tupistraker includes such steps as cutting, pulverizing to obtain powder, extracting in water or organic solvent, concentating or drying to obtain the extract, and sequential extracting by chloroform, ethyl acetate and n-butanol to obtain 3 extracts. They can be used for the frosty mildew of litchi, anthracnoss of banana and frosty mildew of cucumber.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to the preparation and application of a class of plant extracts, in particular to the preparation of the plant extract and its application as a fungicide and preservative in the prevention and control of plant diseases. Background technique [0002] With the continuous development of human society and the further deepening of human cognition, the prevention and control of harmful organisms, overcoming their resistance, ensuring food safety and protecting the environment have received more and more widespread attention. In the field of agriculture, the abuse of chemically synthesized pesticides and their own shortcomings are the main factors leading to resistance of diseases and insect pests, excessive pesticide residues in agricultural products and environmental pollution. In order to solve the above problems, people have increased the development of biopesticides, and plant-de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01N65/42
Inventor 吴光旭陈维信刘爱媛吴振先李雪萍
Owner SOUTH CHINA AGRI UNIV
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