Application of carpesium abrotanoides extracts

A technology of Tianmingjing and extract, applied in the application field of Tianmingjing extract, can solve the problems of human and animal poisoning, environmental pollution of chemical pesticides, unreasonable use of chemical pesticides, etc., and achieve the effect of delaying drug resistance

Inactive Publication Date: 2017-01-11
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to people's unreasonable use of chemical pesticides and the inherent shortcomings of pesticides
As a result, the use of chemical pesticides has caused problems such as environmental pollution, human and animal poisoning, and pesticide residues.

Method used

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  • Application of carpesium abrotanoides extracts
  • Application of carpesium abrotanoides extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of Tianmingjing Antibacterial Active Extract:

[0022] Take 100kg of Tianming’s dry sample, add 70% ethanol for reflux extraction twice, add 800kg of 70% ethanol for the first time, add 600kg of 70% ethanol for the second time, filter, combine the extracts, recover the ethanol from the extracts and concentrate to relative Dilute extract with a density of 1.05, extract the thin extract with ethyl acetate to obtain an ethyl acetate phase, pass the ethyl acetate phase through D101 macroporous adsorption resin, and first elute with 30% ethanol until it is colorless, then dilute this 30% The ethanol eluate was discarded, and then eluted with 85% ethanol until it was colorless, the 85% ethanol eluate was collected, the ethanol was recovered and concentrated into a thick paste with a relative density of 1.25, and dried in vacuum to obtain the antibacterial Extract (according to lactone, the mass fraction is not less than 60%).

Embodiment 2

[0023] Embodiment 2: the preparation steps of Tianmingjing bactericidal microemulsion:

[0024] Add 1.5 parts of Tianmingjing Antibacterial Refined Extract to the adjusted stirring reaction kettle, dissolve 1 part of the pesticide adjuvant Termul5030 with 2 parts of ethyl acetate, stir at a high speed at 40°C to 50°C, and add 3 parts of deionized water, stirred at constant temperature for 1 hour to obtain 30% Tianmingjing bactericidal microemulsion.

Embodiment 3

[0025] Embodiment 3: Determination of Antibacterial Activity of Tianming Fine Microemulsion

[0026] Dilute Tianming Microemulsion 0.02mL to 10mL with melted PDA medium, mix well and pour it into a Petri dish to prepare a plate with bacteria. The pathogenic bacteria to be tested were cultured on a flat plate for 5-7 days in advance, and the fungus cake (diameter: 4mm) was punched on the cultured colony with a sterilized puncher, and then the fungus cake was buckled upside down in the center of the mixed medicine plate, and repeated for each treatment. 3 times. Set an equal amount of sterilized water as a control. The inoculated strain plate was placed in a constant temperature incubator (25±1°C) for cultivation, and the colony diameter was measured for 72 hours by the cross method, and the inhibition rate was calculated according to the following formula. The results are shown in Table 1.

[0027] Colony growth diameter = colony diameter -4

[0028]

[0029] Table 1 Anti...

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Abstract

The invention discloses application of carpesium abrotanoides extracts. A carpesium abrotanoides sterilizing agent is safe for the environment, active compounds in the carpesium abrotanoides sterilizing agent are natural existing substances and derive from the nature, and inherent participation energy and material circulation metabolic pathways are formed in the long-term evolution process, so that no residue is caused when the sterilizing agent is applied to the environment or plants, and no bio-concentration is caused; the sterilizing agent is relatively safe for non-target organisms; the sterilizing agent is different from conventional pesticide in phytopathogen action mechanism, pathogenic bacteria are not likely to generate resistance to the sterilizing agent after the sterilizing agent is applied, and the drug resistance of pathogenic bacteria for conventional sterilizing agents is delayed.

Description

technical field [0001] The invention relates to the application of plant extracts, in particular to the application of Tianmingjing extracts. Background technique [0002] Since the 1940s, chemical pesticides have dominated the pesticide market due to their variety, quick effect, convenient use, and low price. However, due to people's irrational use of chemical pesticides and the inherent shortcomings of pesticides. As a result, problems such as environmental pollution, human and animal poisoning, and pesticide residues have occurred in the use of chemical pesticides. With the improvement of people's quality of life and the enhancement of environmental awareness, the development of bio-pesticides that are safe for humans and animals, non-polluting, low-toxic, and low-residue has become a new hot spot in the field of pesticide research. As a part of biologically rational pesticides, the development and research of botanical pesticides has always been one of the hot topics i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01P3/00
CPCA01N65/00A01N65/12
Inventor 朱红霞胡林峰汤波崔茂金马晶晶李鹏朱英慧陈锡岭
Owner HENAN INST OF SCI & TECH
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