Application of carpesium abrotanoides extracts
A technology of Tianmingjing and extract, applied in the application field of Tianmingjing extract, can solve the problems of human and animal poisoning, environmental pollution of chemical pesticides, unreasonable use of chemical pesticides, etc., and achieve the effect of delaying drug resistance
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Embodiment 1
[0021] Example 1: Preparation of Tianmingjing Antibacterial Active Extract:
[0022] Take 100kg of Tianming’s dry sample, add 70% ethanol for reflux extraction twice, add 800kg of 70% ethanol for the first time, add 600kg of 70% ethanol for the second time, filter, combine the extracts, recover the ethanol from the extracts and concentrate to relative Dilute extract with a density of 1.05, extract the thin extract with ethyl acetate to obtain an ethyl acetate phase, pass the ethyl acetate phase through D101 macroporous adsorption resin, and first elute with 30% ethanol until it is colorless, then dilute this 30% The ethanol eluate was discarded, and then eluted with 85% ethanol until it was colorless, the 85% ethanol eluate was collected, the ethanol was recovered and concentrated into a thick paste with a relative density of 1.25, and dried in vacuum to obtain the antibacterial Extract (according to lactone, the mass fraction is not less than 60%).
Embodiment 2
[0023] Embodiment 2: the preparation steps of Tianmingjing bactericidal microemulsion:
[0024] Add 1.5 parts of Tianmingjing Antibacterial Refined Extract to the adjusted stirring reaction kettle, dissolve 1 part of the pesticide adjuvant Termul5030 with 2 parts of ethyl acetate, stir at a high speed at 40°C to 50°C, and add 3 parts of deionized water, stirred at constant temperature for 1 hour to obtain 30% Tianmingjing bactericidal microemulsion.
Embodiment 3
[0025] Embodiment 3: Determination of Antibacterial Activity of Tianming Fine Microemulsion
[0026] Dilute Tianming Microemulsion 0.02mL to 10mL with melted PDA medium, mix well and pour it into a Petri dish to prepare a plate with bacteria. The pathogenic bacteria to be tested were cultured on a flat plate for 5-7 days in advance, and the fungus cake (diameter: 4mm) was punched on the cultured colony with a sterilized puncher, and then the fungus cake was buckled upside down in the center of the mixed medicine plate, and repeated for each treatment. 3 times. Set an equal amount of sterilized water as a control. The inoculated strain plate was placed in a constant temperature incubator (25±1°C) for cultivation, and the colony diameter was measured for 72 hours by the cross method, and the inhibition rate was calculated according to the following formula. The results are shown in Table 1.
[0027] Colony growth diameter = colony diameter -4
[0028]
[0029] Table 1 Anti...
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