Duranta repens Linn. extract, Duranta repens Linn. bactericidal microemulsion and preparation method of microemulsion
A technology for the extract of False Forsythia, which is applied in the field of False Forsythia extract and microemulsion, to achieve the effects of broad application potential, low cost, and easy availability of raw materials
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Embodiment 1
[0027] Example 1 Preparation of False Forsythia Extract
[0028] Take fresh False Forsythia from the Institute of Spices and Beverages of the Chinese Academy of Tropical Agricultural Sciences, take its stems, leaves, flowers or fruits, dry at room temperature for 5-10 days, and then put them in a constant temperature oven at 55-60°C to dry until the water content reaches If it is less than 10%, pulverize, pass through a 60-mesh sieve, add about 10 times the amount (W / V) of solvent, repeat the extraction 3 times, combine the extracts, dry and concentrate under reduced pressure to obtain the extract of False Forsythia extract.
[0029] The above-mentioned raw materials can also be mixed with multiple stems, leaves, flowers and fruits of False Forsythia, and the operation steps are the same as above.
[0030] 1.1 Selection of extraction solvent
[0031] Accurately weigh 6 parts of equal weight False Forsythia dry powder sample, add respectively acetone, ethyl acetate, absolute e...
Embodiment 2
[0041] Since different extraction processes only affect the extraction degree of False Forsythia, but not the performance of False Forsythia extract, the antibacterial activity of False Forsythia anhydrous ethanol extract on the tested pathogenic bacteria is determined below.
[0042] 2.1 Determination of the mycelial growth inhibitory activity of the tested pathogenic bacteria
[0043] 2.1.1 Tested strains
[0044] Pepper blast fungus (Phytophthora capsici) was provided by the Plant Protection Center of the Spice and Beverage Research Institute of the Chinese Academy of Tropical Agricultural Sciences;
[0045] Fusarium oxysporum f.sp.cubense was provided by the Haikou Experimental Station of the Chinese Academy of Tropical Agricultural Sciences;
[0046] Mango anthracnose fungus (Colletotrichum gloeosporioides) was provided by the Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences.
[0047] 2.1.2 Experimental method
[0048] Us...
Embodiment 3
[0071] Example 3 Preparation of 20% False Forsythia bactericidal microemulsion
[0072] Accurately weigh 20kg of the False Forsythia extract extract prepared in Example 1, be dissolved in the mixed solution of 15kg absolute ethanol and 5kg methanol, fully stir, then add 20kg of emulsifier Tween-20, after stirring evenly, in Under the effect of high shearing machine, gradually add tap water 40kg, stop after stirring at normal temperature for a certain period of time, leave standstill, obtain 20% false forsythia microemulsion.
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