Duranta repens Linn. extract, Duranta repens Linn. bactericidal microemulsion and preparation method of microemulsion

A technology for the extract of False Forsythia, which is applied in the field of False Forsythia extract and microemulsion, to achieve the effects of broad application potential, low cost, and easy availability of raw materials

Inactive Publication Date: 2013-01-23
SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no report on the manufacture of botanical fungicides using the pseudo-forsythia plant as a raw material

Method used

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  • Duranta repens Linn. extract, Duranta repens Linn. bactericidal microemulsion and preparation method of microemulsion
  • Duranta repens Linn. extract, Duranta repens Linn. bactericidal microemulsion and preparation method of microemulsion
  • Duranta repens Linn. extract, Duranta repens Linn. bactericidal microemulsion and preparation method of microemulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of False Forsythia Extract

[0028] Take fresh False Forsythia from the Institute of Spices and Beverages of the Chinese Academy of Tropical Agricultural Sciences, take its stems, leaves, flowers or fruits, dry at room temperature for 5-10 days, and then put them in a constant temperature oven at 55-60°C to dry until the water content reaches If it is less than 10%, pulverize, pass through a 60-mesh sieve, add about 10 times the amount (W / V) of solvent, repeat the extraction 3 times, combine the extracts, dry and concentrate under reduced pressure to obtain the extract of False Forsythia extract.

[0029] The above-mentioned raw materials can also be mixed with multiple stems, leaves, flowers and fruits of False Forsythia, and the operation steps are the same as above.

[0030] 1.1 Selection of extraction solvent

[0031] Accurately weigh 6 parts of equal weight False Forsythia dry powder sample, add respectively acetone, ethyl acetate, absolute e...

Embodiment 2

[0041] Since different extraction processes only affect the extraction degree of False Forsythia, but not the performance of False Forsythia extract, the antibacterial activity of False Forsythia anhydrous ethanol extract on the tested pathogenic bacteria is determined below.

[0042] 2.1 Determination of the mycelial growth inhibitory activity of the tested pathogenic bacteria

[0043] 2.1.1 Tested strains

[0044] Pepper blast fungus (Phytophthora capsici) was provided by the Plant Protection Center of the Spice and Beverage Research Institute of the Chinese Academy of Tropical Agricultural Sciences;

[0045] Fusarium oxysporum f.sp.cubense was provided by the Haikou Experimental Station of the Chinese Academy of Tropical Agricultural Sciences;

[0046] Mango anthracnose fungus (Colletotrichum gloeosporioides) was provided by the Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences.

[0047] 2.1.2 Experimental method

[0048] Us...

Embodiment 3

[0071] Example 3 Preparation of 20% False Forsythia bactericidal microemulsion

[0072] Accurately weigh 20kg of the False Forsythia extract extract prepared in Example 1, be dissolved in the mixed solution of 15kg absolute ethanol and 5kg methanol, fully stir, then add 20kg of emulsifier Tween-20, after stirring evenly, in Under the effect of high shearing machine, gradually add tap water 40kg, stop after stirring at normal temperature for a certain period of time, leave standstill, obtain 20% false forsythia microemulsion.

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Abstract

The invention discloses a Duranta repens Linn. extract; and the preparation method uses the stems, leaves, flowers or fruits of Duranta repens Linn. as raw material and comprises the following steps: using organic solvent to extract and concentrating the extract to obtain Duranta repens Linn. Extract. The extract, solvent and pesticide adjuvant are used to prepare the Duranta repens Linn. bactericidal microemulsion. When the Duranta repens Linn. bactericidal emulsion of the invention is used, water is added and the dilute emulsion is sprayed on the leaves of crops or poured at the boots of crops so that the emulsion is convenient to use; and the emulsion has better control effect on the main diseases of various tropical cash crops and also has prominent protection effect on Phytophthora capsici. The active ingredient of the preparation is natural material, is non-toxic to human and livestock, has no residue and is safe to the nontarget organism; and the processing technology is simple, the raw material is accessible, the cost is low, the product corresponds to the current pesticide development direction and has good development and application potentiality.

Description

technical field [0001] The present invention relates to a plant extract, especially the extract of Forsythia pseudoforsythia, the use of the extract, and the microemulsion composed of the extract. Background technique [0002] At present, as people's ability to research and develop plants is gradually strengthened, it is a research trend in the international development of pesticides to find natural active substances from plants and use them for pest control. According to statistics, there are at least 500,000 different species of plants in the world, including 11,020 species of medicinal plants (including 1,208 subspecies), belonging to 2,313 genera and 383 species. Up to now, 2,400 higher plants with the activity of controlling harmful organisms have been reported. There are more than 1,000 species with insecticidal activity, 94 species with antifungal properties, 11 species with antibacterial properties, and 17 species with antiviral properties. [0003] False forsythia ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/08A01N25/04A01P3/00
Inventor 苟亚峰刘爱勤孙世伟谭乐和李志刚桑利伟
Owner SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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