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Alpha-glucosan originated from Grifola frondosa sporophore and its preparation process and use

A technology of glucan and flower seeds, applied in the field of biomedicine, can solve the problems of no further purification and structure confirmation reports, etc., and achieve the effects of no toxic and side reactions and the best hypoglycemic effect.

Inactive Publication Date: 2009-08-26
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Kubo et al found in the process of extracting polysaccharides from Grifola frondosa fruiting bodies that the water extract (component X) precipitated with 50% ethanol has a hypoglycemic effect, and the main component is glycopeptide. No further purification and structural confirmation reports have been found.

Method used

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  • Alpha-glucosan originated from Grifola frondosa sporophore and its preparation process and use
  • Alpha-glucosan originated from Grifola frondosa sporophore and its preparation process and use
  • Alpha-glucosan originated from Grifola frondosa sporophore and its preparation process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Take 100g of dried Grifola frondosa fruiting body powder and add 500ml of ethanol ether (1:1) mixture, and reflux at 70°C for 6 hours. Centrifuge at 8000rpm to collect the precipitate.

[0053] Add 1000ml of distilled water to the precipitate, and extract at 121°C for 30 minutes. Centrifuge at 8000 rpm, collect the supernatant, add 95% ethanol until the final volume contains 50% alcohol, and obtain a precipitate, which settles at 4°C for 12 hours. Centrifuge at 8000rpm to obtain a precipitate, and wash with absolute ethanol, acetone, and ether in sequence. The resulting sample had a sugar content of 66.7%.

[0054] The precipitate was redissolved in water, 4.0ml was loaded at 50mg / ml, D296 resin was selected, eluted with distilled water at 3ml / min, and the breakthrough peak was collected. Obtained sample 18.7mg, sugar content is 80%.

[0055] Concentrate the above sample and apply HW75 resin at 1ml / min to obtain two adjacent peaks.

[0056] The two peaks of molecul...

Embodiment 2

[0060] Take 100g of dried Grifola frondosa fruiting body powder and add 500ml of ethanol petroleum ether (1:1) mixture, and reflux at 70°C for 6 hours. Centrifuge at 8000rpm to collect the precipitate.

[0061] Add 800ml of distilled water to the precipitate, and extract at 121°C for 30 minutes. Centrifuge at 8000 rpm, collect the supernatant, add 95% ethanol until the final volume contains 50% alcohol, and obtain a precipitate, which settles at 4°C for 12 hours. Centrifuge at 8000rpm to obtain a precipitate, and wash with absolute ethanol, acetone, and ether in sequence. The resulting sample had a sugar content of 70.1%.

[0062] The precipitate was redissolved in aqueous solution, 4.0ml was loaded at 50mg / ml, DEAE-Sepharose Fast Flow resin was selected, eluted with distilled water at 3ml / min, and the breakthrough peak was collected. A sample of 15.3 mg was obtained with a sugar content of 84%.

[0063] Concentrate the above sample and run it on HW75 at 1ml / min to obtain ...

Embodiment 3

[0065] Take 100g of dried Grifola frondosa fruit body powder and add 700ml of ethanol ether (1:1.2) mixture, and reflux at 70°C for 6 hours. Centrifuge at 8000rpm to collect the precipitate.

[0066] Add 800ml of distilled water to the precipitate, and extract at 121°C for 30 minutes. Centrifuge at 8000rpm, collect the supernatant, add 95% ethanol to reach 55% alcohol content in the final volume, obtain a precipitate, and settle at 4°C for 12 hours. Centrifuge at 8000rpm to obtain a precipitate, and wash with absolute ethanol, acetone, and ether in sequence. The resulting sample had a sugar content of 68.1%.

[0067] The precipitate was redissolved in aqueous solution, 4.0ml was loaded at 50mg / ml, DEAE-Sepharose Fast Flow resin was selected, eluted with distilled water at 3ml / min, and the breakthrough peak was collected. A sample of 14.6 mg was obtained with a sugar content of 81%.

[0068] The above sample was concentrated, and Sephacryl S300HR was loaded at 1 ml / min to o...

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Abstract

The invention belongs to the field of biomedicine, and specifically relates to an α-glucan derived from the fruit body of Grifola frondosa, which has a structure of a configuration, a relative molecular weight of 300,000-2 million Daltons, and no ultraviolet characteristics at 200-400nm absorption peak. The invention also discloses its preparation method and its application for preventing and treating diabetes.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an alpha-glucan derived from fruiting bodies of Grifola frondosa. The invention also discloses its preparation method and its use for preventing and treating diabetes. Background technique [0002] Grifola frondosa (Grifola frondosa) is a large fungus belonging to Basidiomycotina, Phytomycotina, Aspertobasidiomycotina, Phyllomycetes, and Polyporus genus taxonomically. It is a medicinal and edible herb Rare edible fungi. Its fruiting bodies are rich in amino acids, polysaccharides and trace elements and other nutrients, among which the frondosa frondosa polysaccharide is the main bioactive component. Grifola frondosa polysaccharide has significant anti-tumor, hypoglycemic, anti-hepatitis, anti-HIV virus, stable blood pressure, improved fat metabolism and immune system functions. Therefore, it has been developed into a variety of health products, mainly as a biological resp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A61K31/716A61K36/07A61P3/10
Inventor 吴梧桐雷红马迅
Owner CHINA PHARM UNIV
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