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Method for dislocation storage of germ resource of chrysanthemum

A technology for in vitro preservation of chrysanthemums, applied in horticultural methods, plant preservation, botanical equipment and methods, etc., can solve the problems of being susceptible to extreme natural disasters and pests and diseases, frequent transfer of tissue culture, normal subculture preservation, preservation work, etc. To prevent the degradation of species and virus infection, to ensure the quality and purity, and to reduce the succession work

Inactive Publication Date: 2007-07-25
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Technical problem The purpose of the present invention is to provide a chrysanthemum test-tube seedling for the current field planting and preservation of chrysanthemum germplasm resources, which has a large workload and is easily affected by extreme natural disasters and pests. The preservation method is used for in vitro preservation of chrysanthemums. Not only the plants grow normally after 10 months of preservation, but also the offspring of the preservation materials have no genetic variation

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  • Method for dislocation storage of germ resource of chrysanthemum

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Embodiment Construction

[0029] The method for preserving the chrysanthemum test-tube seedling provided by the present invention, its embodiment is as follows:

[0030] 1. In vitro preservation of chrysanthemum seedlings in vitro: use 'Olympic Torch' (Dendranthema×grandiflorum Ramat.'Aoyunhuoju') (known and public species, see references Chen Fadi, Fang Weimin, etc. Chrysanthemum new varieties-summer flower type potted small chrysanthemum series . Acta Horticultural Science, 2005, 32(3): 567) varieties were used as test materials. The foot buds of 'Olympic Torch' were taken as explants, sterilized with 75% ethanol for 30 seconds, rinsed twice with sterile water, sterilized with 0.1% mercury chloride for 8 minutes, washed five times with sterile water, and then cut into Stem segments with 1 node in the leaf were placed on MS medium. After 5 days of explant inoculation, the germination rate of axillary buds was 83.3%; 20 days later, the axillary buds were transferred to MS+0.3mg.L -1 6-BA+0.1mg.L -1...

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Abstract

An in vitro storage method for the germplasm resource of chrysanthemum includes such steps as taking the root bud of chrysanthemum as explant, secondary culture of auxiliary bud, and storing in the MS culture medium containing abscisic acid, 6-BA and NAA. Its survival rate after 10 months is 100%.

Description

1. Technical field [0001] The invention relates to a method for in vitro preservation of chrysanthemum germplasm resources, which is specially used for in vitro preservation of chrysanthemum germplasm resources. 2. Technical Background [0002] Chrysanthemum (Dendranthema × grandiflorum Ramat.) is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It has multiple values ​​such as ornamental, edible and medicinal. my country is the origin center of cultivated chrysanthemums and the distribution center of Chrysanthemum germplasm resources. There are more than 20 species of more than 40 species of Chrysanthemum distributed in my country, and more than 3,000 cultivated chrysanthemum varieties. Cultivated chrysanthemums have a high degree of heterozygosity in genetics, and the traits of offspring are separated. It is difficult to preserve germplasm resources through seeds, but asexual reproduction is mainly carried out ...

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01N3/02
Inventor 陈发棣王艳芳房伟民管志勇刘兆磊滕年军陈素梅赵宏波许高娟缪恒彬姜贝贝丁玲
Owner NANJING AGRICULTURAL UNIVERSITY
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