Method for dislocation storage of germ resource of chrysanthemum

A technology for in vitro preservation of chrysanthemums, applied in horticultural methods, plant preservation, botanical equipment and methods, etc., can solve the problems of being susceptible to extreme natural disasters and pests and diseases, frequent transfer of tissue culture, normal subculture preservation, preservation work, etc. To prevent the degradation of species and virus infection, to ensure the quality and purity, and to reduce the succession work

Inactive Publication Date: 2007-07-25
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem The purpose of the present invention is to provide a chrysanthemum test-tube seedling for the current field planting and preservation of chrysanthemum germplasm resources, which has a large workload and is easily affected by extre

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for dislocation storage of germ resource of chrysanthemum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The method for preserving the chrysanthemum test-tube seedling provided by the present invention, its embodiment is as follows:

[0030] 1. In vitro preservation of chrysanthemum seedlings in vitro: use 'Olympic Torch' (Dendranthema×grandiflorum Ramat.'Aoyunhuoju') (known and public species, see references Chen Fadi, Fang Weimin, etc. Chrysanthemum new varieties-summer flower type potted small chrysanthemum series . Acta Horticultural Science, 2005, 32(3): 567) varieties were used as test materials. The foot buds of 'Olympic Torch' were taken as explants, sterilized with 75% ethanol for 30 seconds, rinsed twice with sterile water, sterilized with 0.1% mercury chloride for 8 minutes, washed five times with sterile water, and then cut into Stem segments with 1 node in the leaf were placed on MS medium. After 5 days of explant inoculation, the germination rate of axillary buds was 83.3%; 20 days later, the axillary buds were transferred to MS+0.3mg.L -1 6-BA+0.1mg.L -1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

An in vitro storage method for the germplasm resource of chrysanthemum includes such steps as taking the root bud of chrysanthemum as explant, secondary culture of auxiliary bud, and storing in the MS culture medium containing abscisic acid, 6-BA and NAA. Its survival rate after 10 months is 100%.

Description

1. Technical field [0001] The invention relates to a method for in vitro preservation of chrysanthemum germplasm resources, which is specially used for in vitro preservation of chrysanthemum germplasm resources. 2. Technical Background [0002] Chrysanthemum (Dendranthema × grandiflorum Ramat.) is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It has multiple values ​​such as ornamental, edible and medicinal. my country is the origin center of cultivated chrysanthemums and the distribution center of Chrysanthemum germplasm resources. There are more than 20 species of more than 40 species of Chrysanthemum distributed in my country, and more than 3,000 cultivated chrysanthemum varieties. Cultivated chrysanthemums have a high degree of heterozygosity in genetics, and the traits of offspring are separated. It is difficult to preserve germplasm resources through seeds, but asexual reproduction is mainly carried out ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00C12N5/04A01N3/02
Inventor 陈发棣王艳芳房伟民管志勇刘兆磊滕年军陈素梅赵宏波许高娟缪恒彬姜贝贝丁玲
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap