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A binding domain of bacterial ATP synthase

A 3D structure, binding site technique for identification of antimicrobial compounds, host-vector systems, antimycobacterial agents, treatment of tuberculosis patients, ATPase binding domains

Active Publication Date: 2015-10-21
JANSSEN PHARMA NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these drugs may be effective, structural analogs only temporarily address the problem of resistance (9), as their mechanisms of action are consistent with existing drug families

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  • A binding domain of bacterial ATP synthase
  • A binding domain of bacterial ATP synthase
  • A binding domain of bacterial ATP synthase

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Embodiment approach

[0051] mutant atpE protein

[0052] The present invention provides isolated mutant atpE proteins, specifically bacterial atpE proteins, more specifically mycobacterial atpE proteins, more specifically M. tuberculosis and M. smegmatis atpE proteins. Mutations are selected from single point mutations, insertions or deletions. In one embodiment of the present invention, the mutation includes introducing at least one point mutation at any position (preferably at amino acid 34) at amino acid positions 20-40, especially at amino acid positions 30-40, or introducing at least one point mutation at amino acid positions 60-75. In particular, at least one point mutation is introduced at any position (preferably at amino acid 69) among amino acids 62-73, such as figure 2 The sequence alignment in is shown. In another embodiment, the isolated mutant atpE protein is selected from Mtb_R (SEQ ID No.2), Msm_R09 (SEQ ID No.4) and Msm_R10 (SEQ ID No.5), such as figure 2 shown, or a protein ...

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Abstract

This invention provides an isolated mutant atpE protein and departing from said mutant atpE protein the identification of an ATPase binding domain. This invention also provides related nucleic acids, vectors, host cells, pharmaceutical compositions and articles of manufacture. This invention further provides methods for determining whether a test compound interacts with an atpE protein, i.e. with the ATPase binding domain of the present invention, as well as pharmaceuticals compositions comprising said test compound, in particular as antimicrobials, more particular as antimycobacterial agent, even more particular for treating tuberculosis in a subject.

Description

[0001] The present invention provides isolated mutant atpE proteins and identifies an ATPase binding domain from said mutant atpE proteins. The invention also provides related nucleic acids, vectors, host cells, pharmaceutical compositions and products. The present invention further provides a method for determining whether the test compound interacts with the atpE protein (that is, whether it interacts with the ATPase binding domain of the present invention), and also provides a pharmaceutical composition comprising the test compound, specifically, the The test compounds are used as antimicrobial agents, more specifically as antimycobacterial agents, especially in the treatment of patients suffering from tuberculosis. Background of the invention [0002] Tuberculosis (TB) is the leading cause of adult death worldwide (after AIDS), killing 2-3 million adults each year, and is a key obstacle to efforts to reduce global poverty and disease (1). Factors contributing to the resur...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/35C12N15/31C12N15/52G01N33/50A61K31/00
CPCC07K14/35A61K38/00C12N9/14A61P31/04A61P31/06C12N15/52C12N15/11G01N33/50
Inventor K·J·L·M·安德里斯H·W·H·戈尔曼J·-M·E·F·M·尼弗斯P·K·M·弗哈塞尔特J·温克勒M·R·德琼格L·M·H·科伊曼斯
Owner JANSSEN PHARMA NV