Antitumor antibiotics and its production
An antibiotic and anti-tumor technology, applied in the biological field, can solve the problems of few reports of new compounds, low ability and level of creation, etc.
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Embodiment 1
[0032] Preparation of compound (I)
[0033] (1) Liquid seed culture of strains: ① Preparation of liquid seed medium (g / l): take 4.0 g of yeast extract; 4.0 g of glucose; 5.0 g of malt extract; 1.0 ml of multivitamins; Sterilize at 120°C for 30 minutes; ②Fermentation: Add the strain YIM 80133 to the sterilized medium, and ferment for 2 days at 28°C on a shaking table with a rotation speed of 220 rpm.
[0034] (2) Strain fermentation and cultivation: ① Preparation of fermentation medium (g / l): Take 20.0g of soybean powder; 20.0g of mannitol; Add the seed solution of the bacterial strain YIM 80133 obtained in method (1) to the sterilized medium, the amount of bacteria added is 10% of the weight of the fermentation medium, shake the flask on a shaker with a speed of 220 rpm at 28°C Fermentation for 6 days.
[0035] (3) Treatment of fermented liquid: 1. the fermented liquid is centrifuged at 3000 rpm, and the centrifugation time is 20 minutes; 2. extracted 5 times with equal volu...
Embodiment 2
[0046] The inhibitory activity of the compound (I) prepared in Example 1 on tumor cells was determined by conventional MTT method.
[0047] Experiments have proved that compound (I) has inhibitory effect on human tumor cell lines.
[0048] IC of human promyelocytic leukemia cell line HL-60 50(ug / ml) was 0.23.
[0049] IC of human lung adenocarcinoma cell line AGZY 50 (ug / ml) was 19.58.
[0050] The above tumor cell lines were all preserved by Kunming Medical College.
Embodiment 3
[0052] Preparation of compound (I)
[0053] (1) Strain liquid seed culture:
[0054] ①Preparation of liquid seed medium (g / l): Take 2.0g of yeast extract; 2.0g of glucose; 3.0g of malt extract; 0.5ml of multivitamin; The strain YIM 80133 was added to the sterilized medium, and the flask was fermented for 2 days at 28° C. on a shaker with a rotation speed of 190 rpm.
[0055] (2) Strain fermentation culture:
[0056] ①Preparation of fermentation medium (g / l): Take 10.0g of soybean powder; 10.0g of mannitol; add water to 1000ml, pH 9.0, and sterilize at 120°C for 30min; ②Fermentation: The strain obtained in the above method (1) The seed liquid of YIM 80133 was added to the sterilized culture medium, the amount of bacteria added was 10% of the weight of the fermentation medium, and the flask was fermented for 5 days at 28° C. on a shaker with a rotation speed of 190 rpm.
[0057] (3) Treatment of fermented liquid: 1. the fermented liquid is centrifuged at 2500 rpm, and the cen...
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