Antitumor antibiotics and its production

An antibiotic and anti-tumor technology, applied in the biological field, can solve the problems of few reports of new compounds, low ability and level of creation, etc.

Inactive Publication Date: 2007-11-21
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Antibiotic research in my country is mainly based on tracking and imitation, the ability and level of self-dev...

Method used

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  • Antitumor antibiotics and its production
  • Antitumor antibiotics and its production
  • Antitumor antibiotics and its production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of compound (I)

[0033] (1) Liquid seed culture of strains: ① Preparation of liquid seed medium (g / l): take 4.0 g of yeast extract; 4.0 g of glucose; 5.0 g of malt extract; 1.0 ml of multivitamins; Sterilize at 120°C for 30 minutes; ②Fermentation: Add the strain YIM 80133 to the sterilized medium, and ferment for 2 days at 28°C on a shaking table with a rotation speed of 220 rpm.

[0034] (2) Strain fermentation and cultivation: ① Preparation of fermentation medium (g / l): Take 20.0g of soybean powder; 20.0g of mannitol; Add the seed solution of the bacterial strain YIM 80133 obtained in method (1) to the sterilized medium, the amount of bacteria added is 10% of the weight of the fermentation medium, shake the flask on a shaker with a speed of 220 rpm at 28°C Fermentation for 6 days.

[0035] (3) Treatment of fermented liquid: 1. the fermented liquid is centrifuged at 3000 rpm, and the centrifugation time is 20 minutes; 2. extracted 5 times with equal volu...

Embodiment 2

[0046] The inhibitory activity of the compound (I) prepared in Example 1 on tumor cells was determined by conventional MTT method.

[0047] Experiments have proved that compound (I) has inhibitory effect on human tumor cell lines.

[0048] IC of human promyelocytic leukemia cell line HL-60 50(ug / ml) was 0.23.

[0049] IC of human lung adenocarcinoma cell line AGZY 50 (ug / ml) was 19.58.

[0050] The above tumor cell lines were all preserved by Kunming Medical College.

Embodiment 3

[0052] Preparation of compound (I)

[0053] (1) Strain liquid seed culture:

[0054] ①Preparation of liquid seed medium (g / l): Take 2.0g of yeast extract; 2.0g of glucose; 3.0g of malt extract; 0.5ml of multivitamin; The strain YIM 80133 was added to the sterilized medium, and the flask was fermented for 2 days at 28° C. on a shaker with a rotation speed of 190 rpm.

[0055] (2) Strain fermentation culture:

[0056] ①Preparation of fermentation medium (g / l): Take 10.0g of soybean powder; 10.0g of mannitol; add water to 1000ml, pH 9.0, and sterilize at 120°C for 30min; ②Fermentation: The strain obtained in the above method (1) The seed liquid of YIM 80133 was added to the sterilized culture medium, the amount of bacteria added was 10% of the weight of the fermentation medium, and the flask was fermented for 5 days at 28° C. on a shaker with a rotation speed of 190 rpm.

[0057] (3) Treatment of fermented liquid: 1. the fermented liquid is centrifuged at 2500 rpm, and the cen...

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Abstract

A pyranyl nequinone anti-neoplastic antibiotic is prepared by fermenting, culturing Nocardiopsis sp. YIM 80133, separating out from fermented liquid, purifying extract to obtain final product with molecular formula C21H20010 and molecular weight 432.38. It has strong inhibiting function of human progranuleukemic tumor cell.

Description

technical field [0001] The invention relates to a novel antitumor antibiotic and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Malignant tumor is a kind of worldwide disease that seriously threatens human life and quality of life, and it is the second leading cause of death after cardiovascular disease. Over the years, a large number of anti-tumor active substances have been discovered from microbial metabolites, and a large number of anti-cancer agents derived from microorganisms are increasingly playing an important role in the pharmaceutical industry. Actinomycetes are a kind of microbial resources with large quantity and variety, important practical value and development potential, and are important producers of various physiologically active substances. Antitumor antibiotics produced by actinomycetes, such as doxorubicin, mitomycin, bleomycin, and dactinomycin, are currently commonly u...

Claims

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Application Information

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IPC IPC(8): C07D493/10C12P17/06A61K31/352A61P35/00C12R1/365
Inventor 李一青文孟良李铭刚崔晓龙
Owner YUNNAN UNIV
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