Root culture medium and the culture method in the purple leaf oxalis

A technology for rooting culture medium and sorrel, applied in the biological field, can solve the problems of deformed roots affecting the transplanting survival rate, low transplanting survival rate, and deformed roots, etc., so as to improve the transplanting survival rate and cultivation cost. Low, low cost effect

Inactive Publication Date: 2011-01-19
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, the main problem that exists in this method is: because the rooting medium used is 1 / 2MS+NAA 2.5mg / l+IBA 1.0mg / l, wherein the concentration of NAA is too high, causes abnormal root to appear, transplanting survival rate At the same time, if the concentration of IBA is too high, it will also cause deformed roots and affect the survival rate of transplanting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The young leaves of Oxalis oxalis imported from Japan were used as explants for plant tissue culture experiments, and the young leaves were cut into 0.5cm 2 , inoculated on the induction medium of MS basic medium + 6-BA 1.0mg / L + NAA 0.5mg / L, under the light condition of light intensity 1500-2000lux, light for 10 hours a day, and culture temperature at 20-25℃ , after 5 weeks, the callus was cut into 0.5mm 3 Blocks, placed on the differentiation medium, the formula of the differentiation medium is MS basic medium + 6-BA0.5mg / L + NAA 0.5mg / L, after 14 days of culture, small green buds grow on the callus, and after 40 days The clustered seedlings are formed, and the clustered seedlings are subcultured twice on the above-mentioned differentiation medium to increase the number of seedlings and increase the height. When the seedlings grow to 3 centimeters, cut off the whole root and inoculate in the rooting medium, the rooting medium is 1 / 2MS basic medium+NAA 0.5mg / L+IBA 0.2...

Embodiment 2

[0045] Cut the young leaves of purple-leaved wood sorrel into 0.5cm 2 Inoculated on MS basic medium + 6-BA 1.5mg / L + NAA 0.3mg / L, the callus began to grow after 2 weeks. Under the light conditions of 1500-2000 lux light intensity, 12 hours of light every day, the culture is carried out under the condition of 20-25°C. After 5 weeks, the calli were covered with inoculated leaves, and at this time these callus were cut into 0.5mm 3 Small pieces, inoculated on the differentiation medium of MS basic medium + 6-BA 0.8mg / L + NAA 0.3mg / L for culture. After 14 days, small green buds will grow on the callus, and after 40 days, clusters of seedlings will form. . Carry out two subcultures on the above differentiation medium to increase the number of clustered seedlings and increase the height of the seedlings. When the seedlings grow to a height of 3 cm, cut them off at the root and transfer to 1 / 2 MS basic medium + NAA1 .5mg / L+IBA 0.5mg / L rooting medium. Two weeks later, the test-tub...

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Abstract

The invention relates to a rooting medium of violet leaf creeping oxalis herb tissue culture method and the tissue culture method thereof, wherein the rooting medium comprises 1 / 2MS minimal medium, with 0.2-2.0mg / l of NAA and 0.2-0.8 mg / l of IBA. The tissue culture method comprises inoculation, induction culture, successive transfer culture, rooting culture and test-tube plantlet transplant. The inoculation procedure adopts the young leaf blade of the violet leaf creeping oxalis herb as explant. The tissue culture method of the invention has low cost, high differentiated frequency, high rate of emergence and high survival rate of transplant, is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a rooting medium used in the tissue culture method of Oxalis oxalis and a method for using the rooting medium to carry out tissue culture. Background technique [0002] Purple-leaf wood sorrel (Oxalistriangu laris) is a perennial herb of the Oxalis family. The whole body of the plant is rose-purple, especially the brightest leaves, which are of great ornamental value. At the same time, there are small pink flowers in spring, summer and autumn, which can be used as purple ground cover plants in landscaping, and can also be placed in flower beds or indoors as potted flowers, with a wide range of uses. Although landscaping and the market all need large quantities of Oxalis oleifera, Oxalis oleifera has no seeds because of its flower abortion, so it brings difficulties to the large-scale propagation of this excellent flower. [0003] "Studies on In Vitro Culture and Rapid Propagation of O...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 鹿金颖张纯花潘毅薛淮刘敏
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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